LOCUS       sequence-353008-        8808 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..153
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(173..192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(174..192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(202..219)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(202..218)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(211..233)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      360..815
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(447..466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     657..678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        841..945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(946..1014,1015..1806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1164..1183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1977..2565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2466..2485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2719..2736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2853..2874
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2889..2894,2895..2912,2913..2919)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2927..2943
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2932..2954
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2951..2967
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2951..2967
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2986..3006
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2988..3006
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3034..3260
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3261..3441
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3488..3613
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4106..4339
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4524..4568
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4866..5114
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4866..4886
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5037..5056
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5133..5208
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5256..5373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5513..6691
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5744..6682
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6639..6659
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6740..6749
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6806..6850
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6869..6910
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6920..7627
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7065..7083)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7333..7352
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7697..8285
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7750..7770)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8168..8179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8360..8593
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8665..8786
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8702..8721)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8756..8775
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
       61 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      121 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
      181 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
      241 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      301 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
      361 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
      421 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
      481 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
      541 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
      601 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
      661 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
      721 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
      781 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
      841 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      901 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      961 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1021 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1081 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1141 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1201 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1261 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1321 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1381 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1441 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     1501 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     1561 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     1621 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     1681 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     1741 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     1801 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     1861 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     1921 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2821 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     2881 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     2941 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     3001 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     3061 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     3121 catggcgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     3181 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     3241 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     3301 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3361 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3421 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     3481 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     3541 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     3601 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     3661 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     3721 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     3781 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     3841 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     3901 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     3961 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     4021 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     4081 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     4141 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     4201 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     4261 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     4321 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     4381 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     4441 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     4501 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     4561 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     4621 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     4681 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     4741 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     4801 gattagtgaa cggatctcga cggtatcgat cacgagacta gtatcgagcg gccgccccct
     4861 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
     4921 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
     4981 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
     5041 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     5101 aaggacgaaa caccgggtct tcgagaagac ctgttttaga gctagaaata gcaagttaaa
     5161 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttaattc
     5221 tcgacctcga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5281 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5341 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5401 ccactttggc cgcggctcga ggcgataagc tttgcaaaga tggataaagt tttaaacaga
     5461 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg
     5521 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt
     5581 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt
     5641 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc
     5701 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg
     5761 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac
     5821 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc
     5881 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg
     5941 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa
     6001 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag
     6061 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg
     6121 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc
     6181 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc
     6241 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg
     6301 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg
     6361 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc
     6421 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc
     6481 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc
     6541 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt
     6601 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca
     6661 aagttttttt cttccatttc aggtgtcgtg aggaattctg cagtcgatcg acggtaccgc
     6721 gggcccggga tccacggtcg ccaccatggg cgaggaccag gtggacccca ggctgatcga
     6781 cggcaaggga ggaggcgggg gagggaagga gaccgccgcc gccaagttcg agaggcagca
     6841 catggacagc ggaggaggcg ggggaggggg caaaccaatt ccaaacccac tgctgggact
     6901 ggattccaca ggcatgctga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
     6961 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     7021 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     7081 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     7141 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     7201 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     7261 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     7321 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     7381 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     7441 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     7501 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     7561 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     7621 gtacaagaaa cggccagccg aggatatgga ggaagaacaa gcctttaaac gatctcgata
     7681 aagcggccgc gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7741 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7801 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7861 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7921 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7981 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     8041 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     8101 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     8161 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     8221 gcctcttccg catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     8281 cccgcctggg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     8341 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagata agatctgctt
     8401 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8461 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8521 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8581 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8641 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     8701 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     8761 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcc
//
