LOCUS       sequence-353018-        9880 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9880)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1568
FEATURES             Location/Qualifiers
     source          1..9880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2818..3406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2871..2891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3289..3300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3478..3711
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3789..3910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3826..3845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3880..3899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3950..4085
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4105..4124)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4106..4124)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4134..4151)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4134..4150)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4143..4165)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4292..4747
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4379..4398)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4589..4610
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4773..4877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4878..4946,4947..5738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5096..5115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5909..6497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6398..6417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6651..6668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6785..6806
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6821..6826,6827..6844,6845..6851)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6859..6875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6864..6886
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6883..6899
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6883..6899
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6918..6938
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6920..6938
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6966..7192
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7193..7373
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7420..7545
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8038..8271
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8456..8500
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8731..8941
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8753..8949
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8800..8935
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8981..9583
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8981..9000)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9477..9497
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9626..9743
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ggaagaatca gctgcacaat taggttgttg tcataggccc atggccctgg gaggtacagg
     1261 gggctccctg tcccccagcc tggacttcca gctcttccga ggtgaccagg tcttctcagc
     1321 ctgcagacca cttccagaca tggtggatgc tcatggccca tcctgtgcca gctggctgtg
     1381 tcccttgccc ctggcaccgg gcaggtctgc actgctggcc tgcctacagg acctggacct
     1441 gaacctgtgc accccacagc cggcacccct gggcacagac ctgcagggcc tccaagagga
     1501 cgccttgagc atgaagcacg agccaccagg gctgcaggcc agctccaccg atgacaagaa
     1561 attcacagtc aagtacccac agaacaagga caagctggga aaacagccag aaagagctgg
     1621 cgagggggcc ccctgcccag ccttctcctc tcataacagc tcttccccac caccgctgca
     1681 gaacagaaag agccccagcc ccttggcttt ctgcccctgt ccccctgtca actccatctc
     1741 caaggagctc ccatttctcc tccacgcctt ctaccctgga tacccacttc tcctgcctcc
     1801 accccacctg ttcacctatg gggccctacc ttctgaccaa tgtccccacc tcctcatgct
     1861 gccccaagac ccctcctacc ccaccatggc tatgcctagc ctgctgatga tggtcaatga
     1921 gctggggcac cccagcgctc ggtgggagac cctgcttccc tacccagggg ccttccaagc
     1981 ctctggccaa gctctgcctt cccaggcccg aaatccaggt gctggagctg ccccaaccga
     2041 ctccccaggc ctggagcgtg gtggcatggc atctccagca aagcgggtcc cattgagttc
     2101 ccagacaggc accgcagcct tgccttaccc gctgaaaaag aagaatggca aaatcctgta
     2161 cgagtgcaac atatgtggca agagctttgg gcagctctcc aatctcaagg tccacctgcg
     2221 tgtgcacagt ggagagcgtc cattccagtg tgccttgtgc cagaagagct tcactcaact
     2281 tgcccacctg cagaagcacc acctggtgca cactggggag cggccccaca agtgctcggt
     2341 gtgccacaag cgcttcagca gctccagtaa cctcaagacc cacctgcgcc tgcactccgg
     2401 ggcccggccc ttccagtgca gtgtctgccg gagtcgcttc acccagcaca tccacctgaa
     2461 gctgcaccat cggctgcatg ccccacagcc ctgtggcctg gtgcacaccc agctgcccct
     2521 ggcctctctg gcctgccttg cccaatggca ccagggggca ctagatctta tggcggtggc
     2581 atctgagaaa cacatgggct atgacataga tgaggtcaaa gtgtcctcga catcccaggg
     2641 gaaagcaaga gcagtgagcc tgagcagtgc cgggactccc ctggtgatgg ggcaggacca
     2701 gaacaattag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     2761 atcttgtgga aaggacgagg tgactcgcgt gaacatatga aaacgcgtta agtcgacaat
     2821 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2881 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2941 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3001 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3061 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3121 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3181 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3241 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3301 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3361 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3421 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3481 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     3541 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3601 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3661 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     3721 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3781 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     3901 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     3961 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4021 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4081 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4141 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4201 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4261 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4321 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4381 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4441 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4501 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4561 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4621 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4681 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     4741 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5101 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     6721 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     6781 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     6841 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     6901 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     6961 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7021 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7081 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7141 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7201 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7261 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7321 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7381 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7441 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7501 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     7561 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     7621 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     7681 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     7741 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     7801 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     7861 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     7921 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     7981 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8041 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8101 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8161 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8221 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8281 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8341 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8401 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8461 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     8521 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     8581 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     8641 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     8701 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     8761 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8821 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8881 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8941 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9001 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9061 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9121 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9181 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9241 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9301 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9361 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9421 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9481 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9541 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
     9601 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     9661 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9721 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
     9781 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
     9841 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
