LOCUS       sequence-353435-        5947 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Insect cell expression of GCP5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Wieczorek M, Ti SC, Urnavicius L, Molloy KR, Aher A, Chait BT, 
            Kapoor TM
  TITLE     Biochemical reconstitutions reveal principles of human gamma-TuRC 
            assembly and function.
  JOURNAL   J Cell Biol. 2021 Mar 1;220(3). pii: 211719. doi: 
            10.1083/jcb.202009146.
  PUBMED    33496729
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-03-1"; volume: "220(3). pii: 211719. doi"; 
            pages: " 10.1083/jcb.202009146"
COMMENT     Sequence Label: pACEBac1-GCP5
FEATURES             Location/Qualifiers
     source          1..5947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1906..1911,1912..1928,1929..1934))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2005..2038)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2115..2206
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2131..2148
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    5527..5661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5564..5583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5618..5637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5782..5947
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca taacatcaaa
     1741 catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa
     1801 aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc
     1861 aaggttctgg accagttgcg tgagcgcata cgctacttgc attacagttt acgaaccgaa
     1921 caggcttatg tcaactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca
     1981 accttgggca gcagcgaagt cgccataact tcgtatagca tacattatac gaagttatct
     2041 gtaactataa cggtcctaag gtagcgagtt taaacactag tatcgattcg cgacctactc
     2101 cggaatatta atagatcatg gagataatta aaatgataac catctcgcaa ataaataagt
     2161 attttactgt tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc
     2221 ataccgtccc accatcgggc gcggatcccg gtccgaagcg cgcggaattc aaaggcctac
     2281 gtcgacatgg cgcggcacgg gccaccgtgg agtcggttgg acgcgcagca ggagcgcgac
     2341 gtgcgggagc tcgtccgggg tgtcgccggc ctccaggacg aggcagaccc caacttccag
     2401 ctcgccctaa acttcgcctg gtccaacttc agatttcatc gtttcttgga tgtcaacagc
     2461 cacaaaatag aaaaaacaat cgaaggaatt tatgaaaaat ttgtcattca ttctgatcta
     2521 agcaaagctg ctagttggaa gagattaacg gaggaatttc taaatgcacc acttcccagt
     2581 ataaaggaaa taaagacaga tgcacattat tccatactgt cacttcttct gtgtctgtca
     2641 gactctcctt caaacagcag ttatgtggag acaccaagaa ataaagaagt ggaaaagaaa
     2701 gatgatttcg actggggaaa atacttgatg gaagatgaag aaatggacat tggtccgtac
     2761 atggacacac caaattggtc tgaagaaagt gaagaggaaa atgatcaaca gcccttaagc
     2821 agagaggact ctggaattca ggtagacagg acaccgttag aagaacaaga tcaaaacgga
     2881 aaactggatc cttgtatcag ttggaaagat gagccagatg accgaagctg gctggaacat
     2941 catgtggtcc atcagtactg gacagccagg ccctcccagt ttcctcatag tttacatttg
     3001 cactctaatt tagctgctgt ctgggaccaa cacttgtaca gcagtgatcc attgtatgtt
     3061 ccagatgaca gggttttggt tactgagact caggttattc gggaaaccct atggttactt
     3121 tcaggagtga aaaagctctt tatatttcag ttgatagatg ggaaggtaac tgtgagaaac
     3181 aatattatag taactcattt aacacatagc tgtttacgat ctgtgctgga acaaatagca
     3241 gcatatggcc aggttgtgtt tcgactccag gagttcattg atgaagtcat gggacacagt
     3301 tctgagagca tgctgcctgg aagtgggtct gttcctaaga agtcaactga agctcccttt
     3361 agaacctacc aggctttcat gtgggccctg tacaaatatt tcattagttt caaagaggaa
     3421 cttgcagaaa ttgagaagtg catcatcaat aatgatacta caataactct tgcaatagtg
     3481 gtggacaagt tggcacctcg attgtctcag ctcaaggttc tgcacaaagt gtttagtact
     3541 ggagtagcag aagttccacc tgatactcga aatgtcgtcc gggcctctca cctgcttaac
     3601 accctgtaca aggccattct tgaatatgac aatgttggag aagcctctga gcaaaccgtc
     3661 tccctccttt tctctctctg ggtggaaacg gtgcggcctt acctgcagac ggtggacgag
     3721 tggatcgtgc acgggcacct gtgggatggc gccagggagt tcatcatcca gagaaacaaa
     3781 aatgttccag ttaatcacag agacttctgg tatgcaactt acacgttata tagcgtatca
     3841 gaaaagacag aaaatgaaga aaaaatgagt gataacgcta gtgcgagttc cggcagtgac
     3901 caggggccct ccagcaggca gcacaccatg gtgtccttcc tcaaacctgt cctgaagcag
     3961 atcataatgg ctggcaagtc gatgcagctg ctgaagaacc tgcagtgtgc ggagagcacc
     4021 acctgccagg caggagccag agatgcagaa agaaaaagtt tatacactct ctttctggaa
     4081 tctgtacagt cccgtcttcg acatggagaa gattccactc cacaggttct tactgagcaa
     4141 caggcaacca aggagaacct gatgaagatg cagtccattg ctgaaagcca tcttgaactg
     4201 gatgatgttc atgatccact gcttgccatt aactttgcaa ggatgtattt ggagcagagt
     4261 gactttcacg agaagtttgc tggtggtgat gtatgtgtgg atagatcatc ggaatctgtg
     4321 acatgccaga cttttgaatt aacgctgaga tcctgcctct atcctcatat tgacaagcag
     4381 tatctagatt gctgtggaaa tctcatgcaa actctaaaaa aagattacag gttggtagaa
     4441 tacttgcaag ctatgaggaa ttttttctta atggaaggag gagataccat gtatgacttc
     4501 tacacgtcaa tttttgataa aataagagaa aaggaaacat ggcagaatgt gtcttttctt
     4561 aatgtccaac tccaagaagc agtaggacag cgttatcctg aagatagttc acgtctatct
     4621 atatcttttg aaaatgttga cacagctaag aagaagctgc ctgttcatat cttagatggt
     4681 ctgaccctca gctacaaggt cccatggccc gtggacattg ttataagttt ggaatgtcaa
     4741 aaaatttata atcaagtgtt tcttctctta ttgcaaataa agtgggcaaa atatagtctg
     4801 gatgttttac tttttggtga actggttagt actgcagaaa aaccacgact taaagaaggc
     4861 cttatacatg aacaagacac agttgctcag ttcggaccac aaaaagaacc agtaagacag
     4921 cagattcatc gcatgttcct cttaagagtg aagctcatgc atttcgtgaa cagcttgcac
     4981 aactacatca tgaccaggat tctacacagt acagggctgg agtttcaaca tcaagtcgag
     5041 gaagccaagg atttagatca attgattaaa attcactata ggtatctgtc aaccatccat
     5101 gaccggtgtc tgctgagaga aaaggtcagc tttgtgaaag aagctatcat gaaggtgttg
     5161 aacttggctc tcatgtttgc agacggttgg caggcaggcc tgggcacttg gcgaatggaa
     5221 tctatagaga aaatggaatc tgattttaaa aactgccata tgtttcttgt aaccatttta
     5281 aacaaagccg tctgtagagg atcctttccc catttggaat ctctagcgtt gtcactcatg
     5341 gctggcatgg aacaaagtta agcggccgct ttcgaatcta gagcctgcag tctcgacaag
     5401 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
     5461 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5521 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5581 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5641 gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg ctgctaacaa
     5701 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactat cataacccct
     5761 agggtatacc catctaattg gaaccagata agtgaaatct agttccaaac tattttgtca
     5821 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     5881 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     5941 gcccaca
//
