LOCUS       sequence-35350-e         737 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cre-dependent viral expression of ArchT-tdTomato for neuronal 
            inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, 
            Baratta MV, Winkle J, Desimone R, Boyden ES
  TITLE     A high-light sensitivity optical neural silencer: development and 
            application to optogenetic control of non-human primate cortex.
  JOURNAL   Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13.
  PUBMED    21811444
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Syst 
            Neurosci."; date: "2011"; volume: "5"; pages: "18. Epub 2011 Apr 13"
COMMENT     Sequence Label: pAAV-FLEX-ArchT-tdTomato
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    91..124
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(233..256)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(431..450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     700..718
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 tcgtataaag tatcctatac gaagttatat caaaatagga agaccaatgc ttcaccatcg
       61 acccgaattg ccaagcatca ccatcgaccc ataacttcgt ataatgtatg ctatacgaag
      121 ttatactagg gatcccccct cgagttttac ttgtacagct cgtccatgcc gtacaggaac
      181 aggtggtggc ggccctcgga gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg
      241 gaggtgatgt ccagcttggt gtccacgtag tagtagccgg gcagttgcac gggcttcttg
      301 gccatgtaga tggtcttgaa ctccaccagg tagtggccgc cgtccttcag cttcagggcc
      361 tggtggatct cgcccttcag cacgccgtcg cgggggtaca ggcgctcggt ggaggcctcc
      421 cagcccatgg tcttcttctg cattacgggg ccgtcggggg ggaagttggt gccgcgcatc
      481 ttcaccttgt agatcagcgt gccgtcctgc agggaggagt cctgggtcac ggtcaccaga
      541 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      601 ttgtaatcgg ggatgtcggc ggggtgcttc acgtacgcct tggagccgta catgaactgg
      661 ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg
      721 gtctgggtgc cctcgta
//
