LOCUS       sequence-353608-        5398 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression vector for human IFITM3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Prelli Bozzo C, Nchioua R, Volcic M, Koepke L, Kruger J, Schutz D, 
            Heller S, Sturzel CM, Kmiec D, Conzelmann C, Muller J, Zech F, Braun
            E, Gross R, Wettstein L, Weil T, Weiss J, Diofano F, Rodriguez 
            Alfonso AA, Wiese S, Sauter D, Munch J, Goffinet C, Catanese A, 
            Schon M, Boeckers TM, Stenger S, Sato K, Just S, Kleger A, Sparrer 
            KMJ, Kirchhoff F
  TITLE     IFITM proteins promote SARS-CoV-2 infection and are targets for 
            virus inhibition in vitro.
  JOURNAL   Nat Commun. 2021 Jul 28;12(1):4584.
  PUBMED    34321474
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-07-28"; volume: "12(1)"; pages: "4584"
COMMENT     Sequence Label: pCG_IFITM3
FEATURES             Location/Qualifiers
     source          1..5398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           598..637
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     intron          1097..1669
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(1801..1820)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1866..1921
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1920..1939)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      2275..2410
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2516..2533)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2687..3275)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2767..2786)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3446..4237,4238..4306))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4069..4088
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4307..4411)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4479..4497
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4535..4557)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4657..4676
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(4743..5198)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4880..4901)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5092..5111
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5324..5346
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5338..5355
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5339..5355
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5362..5381
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5362..5380
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgggggat
      601 cttggtggcg tgaaactccc gcacctcttc ggccagcgcc ttgtagaagc gcgtaggcct
      661 tctagaccat gaatcacact gtccaaacct tcttctctcc tgtcaacagt ggccagcccc
      721 ccaactatga gatgctcaag gaggagcacg aggtggctgt gctgggggcg ccccacaacc
      781 ctgctccccc gacgtccacc gtgatccaca tccgcagcga gacctccgtg cccgaccatg
      841 tcgtctggtc cctgttcaac accctcttca tgaacccctg ctgcctgggc ttcatagcat
      901 tcgcctactc cgtgaagtct agggacagga agatggttgg cgacgtgacc ggggcccagg
      961 cctatgcctc caccgccaag tgcctgaaca tctgggccct gattctgggc atcctcatga
     1021 ccattctgct catcgtcatc ccagtgctga tcttccaggc ctatggatag acgcgtcgga
     1081 tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt
     1141 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
     1201 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg
     1261 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag
     1321 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt
     1381 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt
     1441 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg
     1501 ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca tcctgccttt
     1561 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa
     1621 accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc tcctgggcaa
     1681 cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcactcctc aggtgcaggc
     1741 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     1801 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     1861 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     1921 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     1981 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     2041 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     2101 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     2161 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     2221 agctgtccct cttctcttat ggagatccct cgaggagctt tttgcaaaag cctaggcctc
     2281 caaaaaagcc tcttcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg
     2341 cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta
     2401 ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg ctgcattaat
     2461 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     2521 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     2581 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     2641 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2881 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3001 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     3061 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     3121 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     3181 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     3241 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     3301 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     3361 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     3421 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     3481 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     3541 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     3601 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3661 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3721 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3781 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3841 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3901 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3961 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     4021 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4081 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4141 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4201 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4261 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4321 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     4381 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     4441 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4501 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4561 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4621 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4681 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4741 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     4801 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     4861 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     4921 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     4981 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     5041 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     5101 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     5161 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc
     5221 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     5281 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     5341 aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcgccc
//
