LOCUS       sequence-354779-        1782 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  FRET biosensor. The highly sensitive biosensor has a mutated SH2 
            domain, ECFP and YPet.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ouyang M, Wan R, Qin Q, Peng Q, Wang P, Wu J, Allen M, Shi Y, Laub 
            S, Deng L, Lu S, Wang Y
  TITLE     Sensitive FRET Biosensor Reveals Fyn Kinase Regulation by 
            Submembrane Localization.
  JOURNAL   ACS Sens. 2019 Jan 25;4(1):76-86. doi: 10.1021/acssensors.8b00896. 
            Epub 2019 Jan 9.
  PUBMED    30588803
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssensors.8b00896"; journalName: "ACS Sens"; date: 
            "2019-01-25- 25"; volume: "4"; issue: "1"; pages: "76-86"
COMMENT     Sequence Label: Cytosolic Fyn FRET Biosensor
FEATURES             Location/Qualifiers
     source          1..1782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..3,4..6,7..519)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1069..1779
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     LCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLEF
                     LTAAGITEGMNELYK"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacag gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg taccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac
      481 ggcatcaagg cccacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgcccgcatg cattggtatt ttgggaagat cactcgtcgg
      721 gagtccgagc ggctgctgct caaccccgaa aacccccggg gaaccttctt ggtccgggag
      781 agcgagacga caaaaggtgc ctatgccctc tccgtttctg actttgacaa cgccaagggg
      841 ctcaatgtga agcactacaa gatccgcaag ctggacagcg gcggcttcta catcacctca
      901 cgcacacagt tcagcagcct gcagcagctg gtggcctact actccaaaca tgctgatggc
      961 ttgtgccacc gcctgactaa cgtctgtggg tctacatctg gatctgggaa gccgggttct
     1021 ggtgagggtt ctgagaagat cgagggcacg tacggcgtcg tggagctctc taaaggtgaa
     1081 gaattattca ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac
     1141 aaattttctg tctccggtga aggtgaaggt gatgctacgt acggtaaatt gaccttaaaa
     1201 ttactctgta ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttaggt
     1261 tatggtgttc aatgttttgc tagataccca gatcatatga aacaacatga ctttttcaag
     1321 tctgccatgc cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac
     1381 tacaagacca gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta
     1441 aaaggtattg attttaaaga agatggtaac attttaggtc acaaattgga atacaactat
     1501 aactctcaca atgtttacat cactgctgac aaacaaaaga atggtatcaa agctaacttc
     1561 aaaattagac acaacattga agatggtggt gttcaattag ctgaccatta tcaacaaaat
     1621 actccaattg gtgatggtcc agtcttgtta ccagacaacc attacttatc ctatcaatct
     1681 gccttattca aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttttgact
     1741 gctgctggta ttaccgaggg tatgaatgaa ttgtacaaat aa
//
