LOCUS       sequence-354961-       11666 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11666)
  AUTHORS   Landthaler M, Gaidatzis D, Rothballer A, Chen PY, Soll SJ, Dinic L, 
            Ojo T, Hafner M, Zavolan M, Tuschl T
  TITLE     Molecular characterization of human Argonaute-containing 
            ribonucleoprotein complexes and their bound target mRNAs.
  JOURNAL   RNA. 2008 Oct 31. ():.
  PUBMED    18978028
REFERENCE   2  (bases 1 to 11666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Oct 31. ():."
COMMENT     Sequence Label: pDESTmycDICER
FEATURES             Location/Qualifiers
     source          1..11666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..318
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        319..522
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     473..493
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     519..543
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             639..668
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    678..702
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6495..6512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(6539..6563)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6595..6614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6596..6614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6625..6642)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6625..6641)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6634..6656)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     polyA_signal    7046..7180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7083..7102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7137..7156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7306..7761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7393..7412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7603..7624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7878..8235
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7878..8069
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7901..7921)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8086..8221
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8286..9080
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8340..8359)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8950..8969
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9144..9192
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(9233..9252)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9352..9374
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        9498..9602
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9603..9671,9672..10463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9821..9840)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10634..11222
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11123..11142
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11376..11393
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        complement(11467..11571)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattagatgc atgtcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
       61 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      121 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      181 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      241 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      301 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      361 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      421 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      481 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      541 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
      601 agcctccgga ctctagccta ggccgcggta ccaccatgga acaaaaactt atttctgaag
      661 aagatctgtc tagatcaaca agtttgtaca aaaaagcagg ctccaccatg ggaaccaatt
      721 cagtcgacat gaaaagccct gctttgcaac ccctcagcat ggcaggcctg cagctcatga
      781 cccctgcttc ctcaccaatg ggtcctttct ttggactgcc atggcaacaa gaagcaattc
      841 atgataacat ttatacgcca agaaaatatc aggttgaact gcttgaagca gctctggatc
      901 ataataccat cgtctgttta aacactggct cagggaagac atttattgca gtactactca
      961 ctaaagagct gtcctatcag atcaggggag acttcagcag aaatggaaaa aggacggtgt
     1021 tcttggtcaa ctctgcaaac caggttgctc aacaagtgtc agctgtcaga actcattcag
     1081 atctcaaggt tggggaatac tcaaacctag aagtaaatgc atcttggaca aaagagagat
     1141 ggaaccaaga gtttactaag caccaggttc tcattatgac ttgctatgtc gccttgaatg
     1201 ttttgaaaaa tggttactta tcactgtcag acattaacct tttggtgttt gatgagtgtc
     1261 atcttgcaat cctagaccac ccctatcgag aaattatgaa gctctgtgaa aattgtccat
     1321 catgtcctcg cattttggga ctaactgctt ccattttaaa tgggaaatgt gatccagagg
     1381 aattggaaga aaagattcag aaactagaga aaattcttaa gagtaatgct gaaactgcaa
     1441 ctgacctggt ggtcttagac aggtatactt ctcagccatg tgagattgtg gtggattgtg
     1501 gaccatttac tgacagaagt gggctttatg aaagactgct gatggaatta gaagaagcac
     1561 ttaattttat caatgattgt aatatatctg tacattcaaa agaaagagat tctactttaa
     1621 tttcgaaaca gatactatca gactgtcgtg ccgtattggt agttctggga ccctggtgtg
     1681 cagataaagt agctggaatg atggtaagag aactacagaa atacatcaaa catgagcaag
     1741 aggagctgca caggaaattt ttattgttta cagacacttt cctaaggaaa atacatgcac
     1801 tatgtgaaga gcacttctca cctgcctcac ttgacctgaa atttgtaact cctaaagtaa
     1861 tcaaactgct cgaaatctta cgcaaatata aaccatatga gcgacagcag tttgaaagcg
     1921 ttgagtggta taataataga aatcaggata attatgtgtc atggagtgat tctgaggatg
     1981 atgatgagga tgaagaaatt gaagaaaaag agaagccaga gacaaatttt ccttctcctt
     2041 ttaccaacat tttgtgcgga attatttttg tggaaagaag atacacagca gttgtcttaa
     2101 acagattgat aaaggaagct ggcaaacaag atccagagct ggcttatatc agtagcaatt
     2161 tcataactgg acatggcatt gggaagaatc agcctcgcaa caaacagatg gaagcagaat
     2221 tcagaaaaca ggaagaggta cttaggaaat ttcgagcaca tgagaccaac ctgcttattg
     2281 caacaagtat tgtagaagag ggtgttgata taccaaaatg caacttggtg gttcgttttg
     2341 atttgcccac agaatatcga tcctatgttc aatctaaagg aagagcaagg gcacccatct
     2401 ctaattatat aatgttagcg gatacagaca aaataaaaag ttttgaagaa gaccttaaaa
     2461 cctacaaagc tattgaaaag atcttgagaa acaagtgttc caagtcggtt gatactggtg
     2521 agactgacat tgatcctgtc atggatgatg atgacgtttt cccaccatat gtgttgaggc
     2581 ctgacgatgg tggtccacga gtcacaatca acacggccat tggacacatc aatagatact
     2641 gtgctagatt accaagtgat ccgtttactc atctagctcc taaatgcaga acccgagagt
     2701 tgcctgatgg tacattttat tcaactcttt atctgccaat taactcacct cttcgagcct
     2761 ccattgttgg tccaccaatg agctgtgtac gattggctga aagagttgta gctctcattt
     2821 gctgtgagaa actgcacaaa attggcgaac tggatgacca tttgatgcca gttgggaaag
     2881 agactgttaa atatgaagag gagcttgatt tgcatgatga agaagagacc agtgttccag
     2941 gaagaccagg ttccacgaaa cgaaggcagt gctacccaaa agcaattcca gagtgtttga
     3001 gggatagtta tcccagacct gatcagccct gttacctgta tgtgatagga atggttttaa
     3061 ctacaccttt acctgatgaa ctcaacttta gaaggcggaa gctctatcct cctgaagata
     3121 ccacaagatg ctttggaata ctgacggcca aacccatacc tcagattcca cactttcctg
     3181 tgtacacacg ctctggagag gttaccatat ccattgagtt gaagaagtct ggtttcatgt
     3241 tgtctctaca aatgcttgag ttgattacaa gacttcacca gtatatattc tcacatattc
     3301 ttcggcttga aaaacctgca ctagaattta aacctacaga cgctgattca gcatactgtg
     3361 ttctacctct taatgttgtt aatgactcca gcactttgga tattgacttt aaattcatgg
     3421 aagatattga gaagtctgaa gctcgcatag gcattcccag tacaaagtat acaaaagaaa
     3481 caccctttgt ttttaaatta gaagattacc aagatgccgt tatcattcca agatatcgca
     3541 attttgatca gcctcatcga ttttatgtag ctgatgtgta cactgatctt accccactca
     3601 gtaaatttcc ttcccctgag tatgaaactt ttgcagaata ttataaaaca aagtacaacc
     3661 ttgacctaac caatctcaac cagccactgc tggatgtgga ccacacatct tcaagactta
     3721 atcttttgac acctcgacat ttgaatcaga aggggaaagc gcttccttta agcagtgctg
     3781 agaagaggaa agccaaatgg gaaagtctgc agaataaaca gatactggtt ccagaactct
     3841 gtgctataca tccaattcca gcatcactgt ggagaaaagc tgtttgtctc cccagcatac
     3901 tttatcgcct tcactgcctt ttgactgcag aggagctaag agcccagact gccagcgatg
     3961 ctggcgtggg agtcagatca cttcctgcgg attttagata ccctaactta gacttcgggt
     4021 ggaaaaaatc tattgacagc aaatctttca tctcaatttc taactcctct tcagctgaaa
     4081 atgataatta ctgtaagcac agcacaattg tccctgaaaa tgctgcacat caaggtgcta
     4141 atagaacctc ctctctagaa aatcatgacc aaatgtctgt gaactgcaga acgttgctca
     4201 gcgagtcccc tggtaagctc cacgttgaag tttcagcaga tcttacagca attaatggtc
     4261 tttcttacaa tcaaaatctc gccaatggca gttatgattt agctaacaga gacttttgcc
     4321 aaggaaatca gctaaattac tacaagcagg aaatacccgt gcaaccaact acctcatatt
     4381 ccattcagaa tttatacagt tacgagaacc agccccagcc cagcgatgaa tgtactctcc
     4441 tgagtaataa ataccttgat ggaaatgcta acaaatctac ctcagatgga agtcctgtga
     4501 tggccgtaat gcctggtacg acagacacta ttcaagtgct caagggcagg atggattctg
     4561 agcagagccc ttctattggg tactcctcaa ggactcttgg ccccaatcct ggacttattc
     4621 ttcaggcttt gactctgtca aacgctagtg atggatttaa cctggagcgg cttgaaatgc
     4681 ttggcgactc ctttttaaag catgccatca ccacatatct attttgcact taccctgatg
     4741 cgcatgaggg ccgcctttca tatatgagaa gcaaaaaggt cagcaactgt aatctgtatc
     4801 gccttggaaa aaagaaggga ctacccagcc gcatggtggt gtcaatattt gatccccctg
     4861 tgaattggct tcctcctggt tatgtagtaa atcaagacaa aagcaacaca gataaatggg
     4921 aaaaagatga aatgacaaaa gactgcatgc tggcgaatgg caaactggat gaggattacg
     4981 aggaggagga tgaggaggag gagagcctga tgtggagggc tccgaaggaa gaggctgact
     5041 atgaagatga tttcctggag tatgatcagg aacatatcag atttatagat aatatgttaa
     5101 tggggtcagg agcttttgta aagaaaatct ctctttctcc tttttcaacc actgattctg
     5161 catatgaatg gaaaatgccc aaaaaatcct ccttaggtag tatgccattt tcatcagatt
     5221 ttgaggattt tgactacagc tcttgggatg caatgtgcta tctggatcct agcaaagctg
     5281 ttgaagaaga tgactttgtg gtggggttct ggaatccatc agaagaaaac tgtggtgttg
     5341 acacgggaaa gcagtccatt tcttacgact tgcacactga gcagtgtatt gctgacaaaa
     5401 gcatagcgga ctgtgtggaa gccctgctgg gctgctattt aaccagctgt ggggagaggg
     5461 ctgctcagct tttcctctgt tcactggggc tgaaggtgct cccggtaatt aaaaggactg
     5521 atcgggaaaa ggccctgtgc cctactcggg agaatttcaa cagccaacaa aagaaccttt
     5581 cagtgagctg tgctgctgct tctgtggcca gttcacgctc ttctgtattg aaagactcgg
     5641 aatatggttg tttgaagatt ccaccaagat gtatgtttga tcatccagat gcagataaaa
     5701 cactgtatca ccttatatcg gggtttgaaa attttgaaaa gaaaatcaac tacagattca
     5761 agaataaggc ttaccttctc caggctttta cacatgcctc ctaccactac aatactatca
     5821 ctgattgtta ccagcgctta gaattcctgg gagatgcgat tttggactac ctcataacca
     5881 agcaccttta tgaagacccg cggcagcact ccccgggggt cctgacagac ctgcggtctg
     5941 ccctggtcaa caacaccatc tttgcatcgc tggctgtaaa gtacgactac cacaagtact
     6001 tcaaagctgt ctctcctgag ctcttccatg tcattgatga ctttgtgcag tttcagcttg
     6061 agaagaatga aatgcaagga atggattctg agcttaggag atctgaggag gatgaagaga
     6121 aagaagagga tattgaagtt ccaaaggcca tgggggatat ttttgagtcg cttgctggtg
     6181 ccatttacat ggatagtggg atgtcactgg agacagtctg gcaggtgtac tatcccatga
     6241 tgcggccact aatagaaaag ttttctgcaa atgtaccccg ttccccagtg cgagaattgc
     6301 ttgaaatgga accagaaact gccaaattta gcccggctga gagaacttat gacgggaagg
     6361 tcagagtcac tgtggaagta gtaggaaagg ggaaatttaa aggtgttggt cgaagttaca
     6421 ggattgccaa atctgcagca gcaagaagag ccctccgaag cctcaaagct aatcaacctc
     6481 aggttcccaa tagccatcac catcaccatc actgagcggc cgcactcgag atatctagac
     6541 ccagctttct tgtacaaagt ggttgatcgc gtgcatgcga cgtcatagct ctctccctat
     6601 agtgagtcgt attataagct aggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     6661 ctgctagctt gggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     6721 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     6781 taaactagct gcatatgctt gctgcttgag agttttgctt actgagtatg atttatgaaa
     6841 atattataca caggagctag tgattctaat tgtttgtgta ttttagattc acagtcccaa
     6901 ggctcatttc aggcccctca gtcctcacag tctgttcatg atcataatca gccataccac
     6961 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     7021 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     7081 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7141 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatcctgcat taatgaatcg
     7201 gccaacgcgc ggggagaggc ggtttgcgta ttggctggcg taatagcgaa gaggcccgca
     7261 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7381 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7501 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7561 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7621 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7681 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7741 aaatattaac gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     7801 ttcacaccgc atacgcggat ctgcgcagca ccatggcctg aaataacctc tgaaagagga
     7861 acttggttag gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg
     7921 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7981 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     8041 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     8101 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8161 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8221 ctaggctttt gcaaaaagct tgattcttct gacacaacag tctcgaactt aaggctagag
     8281 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     8341 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     8401 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     8461 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     8521 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     8581 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     8641 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     8701 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     8761 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     8821 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     8881 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     8941 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     9001 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     9061 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     9121 caacctgcca tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     9181 gttttttgtg tgaatcgata gcgataagga tccgcgtatg gtgcactctc agtacaatct
     9241 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     9301 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     9361 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     9421 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     9481 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     9541 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     9601 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     9661 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     9721 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     9781 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     9841 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     9901 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     9961 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
    10021 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
    10081 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
    10141 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
    10201 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
    10261 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
    10321 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
    10381 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
    10441 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
    10501 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
    10561 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
    10621 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    10681 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    10741 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
    10801 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
    10861 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
    10921 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
    10981 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
    11041 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
    11101 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    11161 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    11221 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    11281 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
    11341 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
    11401 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagag
    11461 cttgcaattc gcgctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    11521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    11581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    11641 taggcgtatt acgaggccct ttcacg
//
