LOCUS       sequence-35551-e         817 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL2
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..431)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     281..303
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     295..312
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     296..312
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     322..341
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        322..340
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(388..408)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(388..406)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(427..443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(427..443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(440..462)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    451..467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(475..481,482..499,500..505))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cgggggagtt gagaggtccg ccgttacagt tggacacagg ccaacttgtg aagattgcaa
       61 aacgtggcgg cgtgaccgca atggaggcag tgcatgcatc gcgcaatgca ctgacgggtg
      121 cccccctgga gacgggcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
      181 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
      241 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
      301 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg taccgggccc
      361 cccctcgagg tcctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
      421 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      481 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      541 aattgcgttg cgctcactgc ccgctttcca ccggtcgtct ccaacgacca cctcgtcgcc
      601 ttggcctgcc tcggcggacg tcctgccatg gatgcagtga aaaagggatt gccgcacgcg
      661 ccggaattga tcagaagagt caatcgccgt attggcgaac gcacgtccca tcgcgttgcc
      721 gactacgcgc aagtggttcg cgtgctggag tttttccagt gccactccca cccagcgtac
      781 gcatttgatg aggccatgac gcagttcggg atgagca
//
