LOCUS       sequence-35553-e         771 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller 
            JA, Somia NV, Bogdanove AJ, Voytas DF
  TITLE     Efficient design and assembly of custom TALEN and other TAL 
            effector-based constructs for DNA targeting.
  JOURNAL   Nucleic Acids Res. 2011 Jul;39(12):e82. doi: 10.1093/nar/gkr218
  PUBMED    21493687
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2011-07"; volume: "39(12):e82. doi"; pages: " 
            10.1093/nar/gkr21"
COMMENT     Sequence Label: pTAL1
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(12..17,18..35,36..42)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    50..66
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     55..77
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     74..90
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     74..90
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             86..493
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     109..129
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        111..129
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(176..195)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(177..195)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(205..222)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(205..221)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(214..236)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
       61 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
      121 cactaaaggg aacaaaagct ggaggacctc gagggggggc ccggtaccca attcgcccta
      181 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
      241 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
      301 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      361 ggacgcgccc tgtagcggcg cccgtctcca ggggggcacc cgtcagtgca ttgcgcgatg
      421 catgcactgc ctccattgcg gtcacgccgc cacgttttgc aatcttcaca agttggcctg
      481 tgtccaactg taacggcgga cctctcaact cccccgcatc cgtgagcaag gcctccaggg
      541 cgcgtgcgcc ggaccactgt ttgccgacgc caacgatgtc ttcgtgtgtc gcctctggca
      601 acgccgtgat tatgtgctga tacgtgacag cgacggtccc taacgctgcc gggtgttggc
      661 tgagcgcaac gatgtgcgcg tgtgtaaacc catggcccac cagtgcctcg tggtgctgcg
      721 ccactgtcaa acgcaccttc ngtttgatct tctcttgctg ctgctgactg t
//
