LOCUS       sequence-355657-        6312 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Pttr-39::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans D-MN 
            -specific expression of miniSOG-SL2 BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Gao S, Guan SA, Fouad AD, Meng J, Kawano T, Huang YC, Li Y, Alcaire 
            S, Hung W, Lu Y, Qi YB, Jin Y, Alkema M, Fang-Yen C, Zhen M
  TITLE     Excitatory motor neurons are local oscillators for backward 
            locomotion.
  JOURNAL   Elife. 2018 Jan 23;7. pii: 29915. doi: 10.7554/eLife.29915.
  PUBMED    29360035
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-01-23"; volume: "7. pii: 29915. doi"; pages: " 
            10.7554/eLife.29915"
COMMENT     Sequence Label: pJH3458
FEATURES             Location/Qualifiers
     source          1..6312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    519..540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(555..560,561..578,579..585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    593..609
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     598..620
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     617..633
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     617..633
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2021..2341
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     primer_bind     complement(4514..4536)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4636..4655
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4733..4751)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4819..4923
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4924..4992,4993..5784)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5142..5161)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(5955..6312,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      481 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      541 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      601 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa
      661 acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa
      721 tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgg gctgcagcat
      781 gcggccgccc tcttctttct tatcgacagc cattattatt tctatcggct acttttttct
      841 ttttagctgc cttctgctac gcggagaatt ggaaattttc gaaactgatt ttgaatatcg
      901 attctgattg tatttctgat aaagacgtgg aaagttcgaa gggtcggatt acggggtatt
      961 tcaaaggtta cacgacttaa atttaaatag ttttatctat tctcgttggc acgaagtata
     1021 tctaggcaat cgtgacgaag gaaacctttg acgtttgtgt agagaaaagc aaactattct
     1081 ggaacacttc aaaagatctc taaaatacaa atatcgaatt tgtatttttg tatgaaaaag
     1141 tggattaaaa gggtgttcgc ttcgagaaag atacaaaatg ctgaaattag tatatagaag
     1201 ttcctaaagt ttgaaagcag agccaagcgt tactgaattt ggaaaaagga aatttcctgc
     1261 ataggtgaag cctaataggt gaaatggatg taacatgaaa atgttctaaa acattttcac
     1321 cgtaaaattt aggtaggcgg gaaggtcctg ttgtgcccat ataggcttat gatctctctg
     1381 aaaaactctc tgaactttga atttctccag aatgtttttg gtttctgatc gataaatgtt
     1441 tactggttgt tgatgtttgt aaaagtcctg caaattctag aaaattctca aactcttttt
     1501 cttattttca tgagaaaacc gaaactttga agtacataag caaagagttg cgttgttatt
     1561 ttaatctttt gaacctatta ataaatcttt tttgttttct cgggattatg ttaggattac
     1621 aaagacattt aagacaagaa gttgtttctg ttatttcact ttttttgttt gcgcattcac
     1681 aattttgtta cccgggattg gccaaaggac ccaaaggtat gtttcgaatg atactaacat
     1741 aacatagaac attttcagga ggacccttgg ctagcgtcga cggatcccga tgtcggacac
     1801 aattcttggt ttcaacaaat caaacgtcgt tttggctgct ggaattgctg gagccgcttt
     1861 cctcggctac tgcatttact tcgatcataa gagaatcaac gctccagact acaaggacaa
     1921 gattaggcaa agtcagtgtt ttaacaacat atttccttcg gatttttatc taaaaacaac
     1981 ttattttctt tcagagagac gtgcccaggc tggggatcct atggagaaaa gtttcgtgat
     2041 aactgatcca cggctgccag acaatcccat catcttcgca tccgatggct tcctggagct
     2101 gaccgagtat tccagagagg agatcctggg ccgcaatggc cgctttctgc agggaccaga
     2161 gacagaccag gccacagtgc agaagattcg cgatgccatt agagatcagc gcgagattac
     2221 cgtgcagctg ataaactaca caaaaagcgg gaagaaattc tggaacctcc tgcacctcca
     2281 gcccatgagg gaccagaagg gtgagctcca gtatttcatc ggagtgcagc tggatggata
     2341 aagatccccg agatcttcag cgctcagcat tcgtagaatt gtaagtttaa actttctcat
     2401 actaactaac gcgctctatt taaattttca gaattcgctg tctcatccta ctttcaccta
     2461 gttaactgct tgtcttaaaa tctatgcttc tctttagtat ctaaaatttt cctagaagct
     2521 tacaagtata taaatggtct cttctcaata aaggttgtat atttattcat cttattgaat
     2581 ctgccatttc ctcgtttttg cgagtttata taccttccaa ttttctttct attgtatttt
     2641 caacttctaa ttttaattca gggaaactgc ttcaacgcat cggtaccatg gtctccaagg
     2701 gagaggagct cttcaccgga gtcgtcccaa tcctcgtcga gctcgacgga gacgtcaacg
     2761 gacacaagtt ctccgtccgt ggagagggag agggagacgc caccaacgga aagctcaccc
     2821 tcaagttcat ctgcaccacc ggaaagctcc cagtcccatg gccaaccctc gtcaccaccc
     2881 tctcccacgg agtccagtgc ttcgcccgtt acccagacca catgaagcag cacgacttct
     2941 tcaagtccgc catgccagag ggatacgtcc aggagcgtac catcttcttc aaggacgacg
     3001 gaacctacaa gacccgtgcc gaggtcaagt tcgagggaga caccctcgtc aaccgtatcg
     3061 agctcaaggg agtcgacttc aaggaggacg gaaacatcct cggacacaag ctcgagtaca
     3121 acttcaactc ccacaacatc tacatcatgg ccgtcaagca gaagaacgga atcaaggtca
     3181 acttcaagat ccgtcacaac gtcgaggacg gatccgtcca gctcgccgac cactaccagc
     3241 agaacacccc aatcggagac ggaccagtcc tcctcccaga ctcccactac ctctccaccc
     3301 agtccgtcct ctccaaggac ccaaacgaga agcgtgacca catggtcctc ctcgagttcc
     3361 gtaccgccgc cggaatcacc ctcggaatgg acgagctcta caagtgataa gatatcacct
     3421 cccacaacga ggattacact atcgttgagc aatatgaaag agctgaaggg cggcactcga
     3481 caggtggcat ggatgaattg tataagtagg agctcccgag atcttcagcg ctcagcattc
     3541 gtagaattgt aagtttaaac tttctcatac taactaacgc gctctattta aattttcaga
     3601 attccaactg agcgccggtc gctaccatta ccaacttgtc tggtgtcaaa aataataggg
     3661 gccgctgtca tcagagtaag tttaaactga gttctactaa ctaacgagta atatttaaat
     3721 tttcagcatc tcgcgcccgt gcctctgact tctaagtcca attactcttc aacatcccta
     3781 catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa aacttctctt
     3841 aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg tgtagattca
     3901 aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc ccccattaat
     3961 aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt tttttacttc
     4021 tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt atcatatgtt
     4081 acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc tcatgacgtc
     4141 aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa tcaagtgaaa
     4201 gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg tttgtcaaga
     4261 tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg atgcaagaaa
     4321 gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt gataatttga
     4381 aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc ccaatatacc
     4441 aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     4501 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     4561 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     4621 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4681 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg
     4741 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     4801 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     4861 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4921 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4981 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5041 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5101 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5161 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5221 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5281 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5341 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5401 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5461 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5521 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5581 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5641 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5701 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5761 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5821 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5881 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5941 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6001 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6061 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6121 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6181 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6241 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6301 ttggagcgaa cg
//
