LOCUS       sequence-355752-        8747 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Prgef-1 NLF-1 unc-54 3' UTR C.elegans pan-neural expression of NLF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8747)
  AUTHORS   Xie L, Gao S, Alcaire SM, Aoyagi K, Wang Y, Griffin JK, Stagljar I, 
            Nagamatsu S, Zhen M
  TITLE     NLF-1 delivers a sodium leak channel to regulate neuronal 
            excitability and modulate rhythmic locomotion.
  JOURNAL   Neuron. 2013 Mar 20;77(6):1069-82. doi: 
            10.1016/j.neuron.2013.01.018.
  PUBMED    23522043
REFERENCE   2  (bases 1 to 8747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.01"; journalName: "Neuron"; date: 
            "2013-03-20- 20"; volume: "77"; issue: "6"; pages: "1069-82"
COMMENT     Sequence Label: pJH2437
FEATURES             Location/Qualifiers
     source          1..8747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6289..6306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6289..6305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6298..6320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6518..6973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6605..6624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6815..6836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6990..7009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7109..7131
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7169..7187)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7255..7359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7360..7428,7429..8220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7578..7597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8391..8747,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga atggccatgg
      661 gacgtcgacg cttgcatgcc tgcagcgttt ccgatacccc cttatatcag cacacattca
      721 gtcatcatac cacaaaatgt tgtgatagtg ataggcaaaa ctaccgtatt tcctctatta
      781 atcttgcatg caagactaat tttcgattaa cccgtagggg tgcaagacta atagagactg
      841 caagactatt agaggctgaa atactaattt tcgtatgctc aataattttg gaaattggcc
      901 tattttttgt agaaacttga taccgtttaa acaaggaaaa atacacactt tttaatattt
      961 catcaataat ttgaacgatt ttgtgatttt aagttcaatt tgcccaaaaa aagacaattt
     1021 ttctgacgtt accgcaaagt aatgctggtc aggcaaaaag agcggtgcaa aaatataaga
     1081 gactgcaata ctaatagagg aaatacggta attgaatttt agtgaaactt gcgacagttt
     1141 tcctcatttt tttgttattc cggcatacga gtgtggcata cgagtgaggt catcttttgt
     1201 ttcttccgtt tcttcatcgc ttttgaaaaa aaatgttgaa gaaaacttcg tagccgatag
     1261 ccggataatg tttggcacgg cgttccaaca tatatcattg gaaatttttt aagatttttg
     1321 cgaaaaatca cattcttcac gatgagaaca cgttattgaa ggaatataaa tcaagaataa
     1381 catatagtta tatttctcta ttactttaac gttaaatatg agcaaatttg agcattttga
     1441 ttgcgatgaa aagcagaatc gagtcaactg aaatccgttc aaaaacctaa gctcggttcg
     1501 cccgaaagtc gatttttcca atagccgaac agcactgctt ctttattttc caggtatttt
     1561 tggggtgtaa gaattatatc ttctttgttt gggtatttac gtttccgaat tccctttctt
     1621 gatctgtcgc tatttttcag aaaccagaac agtttatcct ttttcatgag aacctgaata
     1681 gctcaaaaac ccgtttaatt tcttttcatc atctcatttg aaactttcct agtcttctta
     1741 atgatttcca ggctcctcct acatttgtat ctcaagatgc atacacttta tttccgctcc
     1801 aattcgaatc atctacttct cttttttctt caactttcta cttttttcca ttcttttctt
     1861 tcaaatgttc gacgtcttca agtgactgct cttcattctc ctcatttttc tcgaatttca
     1921 ttcttgtctc ttttttgctc aaaaatggaa aatgaaagtg acgtgagatt tggacggcgg
     1981 gacacggggg cagtagaagc agcaaaaagg agagaaagag gacacaaata agaagaacga
     2041 attcaaaaat aagcggagag gagctatttc cgtcaattct acctccccaa tcttcatcaa
     2101 ttcggctcaa ttgaatgacg tcacagggag ataaaccgtt tggatgagcg ccgtccatca
     2161 ctgacgccat cccgtttggg acaagaaaag agaaaaaaga gcacaaagtt tttggtgacg
     2221 gatcttgtca atcatatgaa agttgttctg attgattgtc agtttttttc ctactttttt
     2281 ggattctatc cacttctgaa cttttgacaa gtttcaaact ttctgaatca ttttctatgc
     2341 attttcctgg aattcttttt atgtaaaata tgaaatagaa tgtttttgaa ttcaagttct
     2401 gcttttttct tcttttctgt tctgttcggg cttgggtatg ctttttttaa aaattatttt
     2461 gcacatcgac caataagtgc gcaacttata aaattaattt atttttgtta atttttgaaa
     2521 tacttgtatt gctttaagtg atctgacctc gccctgagct ttccacgtag ttatcaaata
     2581 caaatcctcc aagggtaacg tacctatatt actgatcttt ataataactt tatcacctgt
     2641 ccagttccag aggaattctg ttaagcttat aggcacagaa ggagtcattt ctgctggttt
     2701 ttaatgatcc aaatctttat ttcaagtaaa aaactgaaca cttgcgaata aaactatcag
     2761 attaaccatt caccaaaaat gtgtttgaat ctaaaacttt ctcagtattc caaatataga
     2821 aataaataac cacgacattg ctaaaatctg tctgaattgt gtactcctta ccgtgaagta
     2881 ataatggata taatgaatcg tttgaaatga atgatcagca catttttggt gaaagatcac
     2941 aaataaggaa taagcgtcgg aaaataaaac gattatttcg gatcaaaaat ttgttgaaga
     3001 tcatatacac tcgagaccaa gattattcta gacaattttc aaattggctt ctttgtttgc
     3061 aaatcttcat aataatctgt gaagtttgga aatttgaatt tttaatcttt tttcatagat
     3121 tatagttttt atttctttgc aaaactatat taaaaaccga tgcattgttt tagggaaatt
     3181 aatgagcctt ttgttcaaca ctaaaaacaa taaaattaaa attttggctt catcatttga
     3241 ccttttttaa gttcgaaaac ttttctcgta tttctgaacc gccaattttt tcacacatct
     3301 ctagactttt ggtgcccgtt ccagaaagtt aagtaattgc tattctaaga aagttctcaa
     3361 cattgttttt tagttctgat tgaattctga tgttccagga atatatttta aatttaatat
     3421 tctttgcact atttctatac taactaaata ataaatagtc taagtatgtt caatgagaac
     3481 aaaaaagctc tatctatatt tttctatcct atttcattta tatccttttc attttgaact
     3541 caccagcttc tcttcttctt cttgtccata tacttcttaa aagtctcgaa cttctctctc
     3601 tcactctcca tcaatttctt tatggctacc gcttgcgctc tgccgcttcc gaagaaagaa
     3661 ggcgttggca gagctctcaa ttctattttt tctcgccgta ggctatcgat ttctcagctt
     3721 ctcttctctc ttcctcactc caccaccacc acttcgggct tcttcttttc ttcattttcg
     3781 tcttcttctt catcctcttc tttttttttc agttccccct cactcctccc cttatatgcg
     3841 cgtgcgagag gggtgcaaaa gcagcgcgca tccgagaatt gaaacgagaa aacggagacg
     3901 cagcagcagt tcgtcctcag aaaaatagcc agtaaaaaga gaaaaagata gagagagacc
     3961 tatcgcattt tattttcaaa ttgcaattcc tgcaattcat gtgtgcgtgt gcccattttc
     4021 aattcttccc gttatttttc agaccattac caacgttctt tctagatctt gagacaatct
     4081 tccttctgct caatcgttcg tcgtgaagac ggcatcgacg acgacgacga tcagcagtaa
     4141 aggatcctta tgcggcctcg cacgccttac acaaaagaag cgctacgata cgagcgtcgt
     4201 catcaaaagc ctccacccac tcatccgcct gctcctacag ctgctcctcg tccagacact
     4261 acagaagtat tcggccggcg agaagcaaat tcgtcgccga tcaccgattt tatccagtcg
     4321 ttttgtgcga gcattcaaga cgaaagctgc agttcaactg acgagccaaa ctttgaattc
     4381 aaaaatgata ttactgaacg attatggtta aagccttttg atattgtgcc ttcgcctcaa
     4441 attttatccg attcgaagaa aactagttct ccaagaaggt gctgggccaa gaattttcgc
     4501 cgatatttaa ccgatggtat tcttattatt caattattta ctttattcaa ttgcgcttct
     4561 tcatctcatt tatccaacac aattcctcct tgtaaaaatt ttgggttttt tgcgcagcat
     4621 agcgacaatg taactgatcg ttgcacagtt tggtctgatg acgtcattcc tgaaatttgc
     4681 tcagctggca cacaatcacg tattagaata ttaaagcatg tgttcctttt tccagagtta
     4741 caaccgttat cattttttga aatattcgcc gtcggcttga gaaacactga cacgtggcag
     4801 ttgaatgccg gcgagaaggg taatgattgc atttcgggaa gtataaatcg gtgtacatcg
     4861 tgtttccgga ggatctcagg cactcttcgt aagcttaatg aagcctatcg gtctttcgac
     4921 acaactcttt ctcgtttcga ctgccttcca gcggtggaca ctgcctccgc aacaagaccg
     4981 ttctcgccga acgcaacttg tgataattgt aaaatatggt atcgtagatg gttgttggtt
     5041 caatcgcttc aaatctggca acggcctccg tgtattaact ggtgctacta tactcaattg
     5101 gcatgcccac atctcgcacc tgcaaaatta tgggactacg caggtcatcc gtcgtttcag
     5161 tgtagagata tggacattga cagttggcgc cacgaatgtg actgcatcca tccgtgtgat
     5221 gttcgtggaa ttgtagctcc aggtgttgca atgggcaacg ctccttcagt tcagcacgac
     5281 tttttcgcgg cacagattca ctgtgaaacg cggaaaaagg agtgccacaa gcgtttcaaa
     5341 cagattcgga aatcaaaacg gagtgacacc agcagtaaca ttgttttttt atcaagcgca
     5401 tcaccaaaac gcccaaaatt cctgattctt tcattgttgt ttcttcgatt tatgttgttg
     5461 ttataggggg taccatggta ttgatatctg agctccgcat cggccgctgt catcagatcg
     5521 ccatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc
     5581 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt
     5641 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat
     5701 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa
     5761 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata
     5821 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt
     5881 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc
     5941 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta
     6001 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg
     6061 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg
     6121 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg
     6181 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca
     6241 taactgtttc tactagtggc cacgtgggcc gtgcacctta agcttggcac tggccgtcgt
     6301 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6361 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6421 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     6481 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     6541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6721 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6841 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     6901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6961 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7021 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     7081 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     7141 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7201 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7261 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7321 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7381 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7441 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7501 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7561 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     7621 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7681 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7741 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7801 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7861 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7921 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7981 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     8041 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8101 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8161 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8221 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8281 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8341 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8401 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8461 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8521 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8581 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8641 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8701 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaac
//
