LOCUS       sequence-355968-        8600 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Insect Expression of DmNot4-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Keskeny C, Raisch T, Sgromo A, Igreja C, Bhandari D, Weichenrieder 
            O, Izaurralde E
  TITLE     A conserved CAF40-binding motif in metazoan NOT4 mediates 
            association with the CCR4-NOT complex.
  JOURNAL   Genes Dev. 2019 Feb 1;33(3-4):236-252. doi: 10.1101/gad.320952.118. 
            Epub 2019 Jan 28.
  PUBMED    30692204
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2019-02-1"; volume: "33(3-4):236-252. doi"; pages: " 
            10.1101/gad.320952.118. Epub 2019 Jan 28"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmNot4-dsRNAres_Q
FEATURES             Location/Qualifiers
     source          1..8600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2585..2650
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2675..2701
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5906..5947
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5957..5974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5997..6014)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6047..6181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6071..6090)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6125..6144
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6782..7370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6862..6881)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7541..8332,8333..8401))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8164..8183
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8402..8506)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8574..8592
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa
     2641 agctgcaaac ggtggatctc ctccacatat ggcttaccca tacgatgttc cagattacgc
     2701 tcctccaagc ttgaattctg cagatatcca gcacagtggc ggccgctcga gtatgaacgg
     2761 cctgagcagc aatgatgatg cagtcgagtg ccctttgtgt atggagccgc ttgaggtgga
     2821 cgatctgacc ttctttccgt gtacctgcgg atatcagatt tgccgattct gttggcatag
     2881 gatccgcact gatgagaaca agctgtgtcc ggcatgccga aaggagtatc ccgagaatcc
     2941 agctgacttt aagccgctgt cgcaggagga aatgattgcc ttcaagtccc agaaacgcca
     3001 aagggaccaa caacgaaaac aaaagatcac cgagaaccgc aaacacttgg ccaacgttcg
     3061 cgttgtccaa aagaacttgg tgttcgttgt ggggctacca ccacgacttg ctgatgcaga
     3121 catattaaag aaacacgagt atttcggtaa atatgggaaa attcataaag tcgttataaa
     3181 tccaagtacc acgtatgccg gggtccaggg tccatctgct tctgcgtatg tcacatatgt
     3241 taataactcg gatgccttgc gggccattca aagcgtcaat aatattatga tagacggtcg
     3301 gcttataaag accagcttgg ggacaaccaa atactgtagc cacttcatga agaaccagca
     3361 gtgtcccaag agcgactgca tgtacttaca tgaattgggc gatcccgagg ccagtttcac
     3421 aaaggaggaa atgcatcagg gaaagcacct ggagtacgag aagcgcctgc acgatactct
     3481 aattgcgtca ttgggaccga atgcaacagg tattgtaaat gtcgcgattc catcgtcatc
     3541 gtcagcgtca tcctcgtcct ctggatcggg tacaaatggt tcaagtgcat caggtaatgc
     3601 ccagcaaaag gaggcctggc caagcttatc ggtcccgccc atcaatggca aagaggcggc
     3661 tgccactgcg accagttcca gtgggaaaag taaacgggag aagctgcgca acgagaagag
     3721 gcacgagaag aacaaggcga agaataagaa cggcagcaat acaaacgcca atgcctcaaa
     3781 caaggagaac tatgttcccg aaacgagaag cagcacaagt actgagacat tcgcagaggc
     3841 tacggcggat gcaccggctt ccaccaaggc agaaccgccg caagcctcta gcaatagaac
     3901 gagggcggat cgtggaaaag atcggaccac ggctagtgca aaggagcaga agaagggcaa
     3961 ggaaactgct ccagcacctg cagcaagtaa accggcggag cgggttgaaa caagcgagag
     4021 tacaataaga caaaagaagg cggaagtaac cgaaagctgt gaagataact taccacaaaa
     4081 gagattagcg ggaacaaacg ttcaaagatc tgtgagctct tgtagcgaaa atagcgaagg
     4141 acacgtctct gagagtagct taagtgagaa gagtttaact ggtgattatg tggaggaaaa
     4201 gtgcaatagt gtgaattcgg aaagccagca agaaagtgta aagtttcagg aggagctcga
     4261 aaagagcaac gaggcaattg tcgaggccga aacgattctg ccaacagctg agtcctctga
     4321 ggacatcagt cccgctgcag tggcgccagg caatggagaa gacgaaggat gcttacccgt
     4381 tgttgatcca gtcgaaccac cgagtctggc ggataatgga agcagagtaa cagatgcgct
     4441 atctaagctg aacattttcg atgacacgcc tagctttttc acatcgccat cattccagca
     4501 agcacccata ttaaagaata aattggacct ggagatgcgc caatcacacc ttccggatct
     4561 ggtgaatgat atcgacggca ttcagaaagc gagcaacacg aatgaatggg aagaggcttt
     4621 caagaacgtc atgatgggaa atacccaaca tatggaagaa cagttgctgc aacaacagca
     4681 cctgcaacaa caccaaaacc tgcgtcatca attggtcctg cagcaagaag aatttttgcg
     4741 tatgcaggag cttcaaaagc gtaataattt cgctacccag attaatggac cggctaacga
     4801 ctttttgcga gcctacgaat tgcgcgccca ggctaacgcg attatccaac agcagctact
     4861 acagcaacat gcgggagaga acttgttcgg aggaaatatg tccaaatttt ttgactttca
     4921 caagtcgcaa ccccaatcgc atcaccaata ccttaacggc caccccccgc agattaacgg
     4981 taacggagcc gtccccgaac cccagcgagt cgctgcttcg ctggagtcca accgcttgaa
     5041 cagcccgttc gtggagaacg gcttgatcaa tagccaacaa caacaacagc aacagcagaa
     5101 acaacggatg atgggcatgt acgagttcat gccaccgaat acccagagtc aacaaaaccg
     5161 cttcacgcaa aatagcattg tggatgacga tctaggcttt gatcccttcg ttgagaccca
     5221 aaagggactt gctgaactta tggaaaatga ggtggtccaa aaacagagta ttaataatga
     5281 gaaccccttg ccgaagttgc cgccacagcc tcaagttcca ccccatccgc aattggtgga
     5341 caacctgcag cgagctcgca tgccgccgcc aggcttcaat cacgtcaaca cattgggctt
     5401 gggcggtgca tccagactgc agctcaccag caaaataatg cccttcatga acatgcccgt
     5461 taatggggtg ggcaacagcg gtgcccaggg acagcaccaa atgccaatgg gtgtcaactg
     5521 gaatgcgccg atgggcatgc accagaaccc gggacagccc gtgggtgatt cgcaattgca
     5581 gcatccaatg gctcacaaca aggtttacaa caacagtgat tggacttcaa tggatccggc
     5641 tatactttcg tttagacagt attcttcttt tccacaaaac caaattcccc cacatcctca
     5701 gcagcaacag gatttatttt tgcagcattt ggctcaacag caaaattccc agagtggtgg
     5761 tttcaacaac caggcacagc aaatgcttcc tatggggatg cccaatagtt tgctgaacgg
     5821 acaacaaacg cagccgccac aggtcaatgc caatgttcag ggcatgcttg agtttttaaa
     5881 aagccgtcaa ttcgtttagt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     5941 ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     6001 gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat
     6061 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6121 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6181 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     6241 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt cgaccgatgc
     6301 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     6361 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     6421 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
     6481 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
     6541 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct
     6601 tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg
     6661 gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc
     6721 agggacagct tcaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7201 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     7261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     7861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     8161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8521 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     8581 gtatcacgag gccctttcgt
//
