LOCUS       sequence-356190-        8783 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral vector encoding a CRE inducible Katushka gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Bresser K, Kok L, Swain AC, King LA, Jacobs L, Weber TS, Perie L, 
            Duffy KR, de Boer RJ, Scheeren FA, Schumacher TN
  TITLE     Replicative history marks transcriptional and functional disparity 
            in the CD8(+) T cell memory pool.
  JOURNAL   Nat Immunol. 2022 May;23(5):791-801. doi: 
            10.1038/s41590-022-01171-9. Epub 2022 Apr 7.
  PUBMED    35393592
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41590-022-01171-9"; journalName: "Nat Immunol"; date: 
            "2022-05"; volume: "23"; issue: "5"; pages: "791-801"
COMMENT     Sequence Label: pCDHblast-flx-sORF-Katushka
FEATURES             Location/Qualifiers
     source          1..8783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    5..121
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        211..414
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     365..385
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     411..435
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(485..518)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1374..1407)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1430..2137
                     /codon_start=1
                     /product="far-red-shifted derivative of red fluorescent 
                     protein from Entacmaea quadricolor, also known as Katushka 
                     (Shcherbo et al., 2007)"
                     /label=TurboFP635
                     /note="mammalian codon-optimized"
                     /translation="MVGEDSVLITENMHMKLYMEGTVNDHHFKCTSEGEGKPYEGTQTM
                     KIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERITTYEDGG
                     VLTATQDTSLQNGCLIYNVKINGVNFPSNGPVMQKKTLGWEASTEMLYPADSGLRGHSQ
                     MALKLVGGGYLHCSLKTTYRSKKPAKNLKMPGFYFVDRRLERIKEADKETYVEQHEMAV
                     ARYCDLPSKLGHS"
     promoter        2155..2654
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2190..2213)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2587..2606
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2670..2679
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2676..3074
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3081..3669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3134..3154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3552..3563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3743..3976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4048..4169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4085..4104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4139..4158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4188..4323
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4356..4372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4356..4372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4369..4391)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4380..4396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4404..4410,4411..4428,4429..4434))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4449..4470
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4587..4604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4758..5346)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5517..6308,6309..6377))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6140..6159
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6378..6482)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6550..6568
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6606..6628)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6728..6747
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6941..6963
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6955..6972
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6956..6972
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6987..7213
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7214..7394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7441..7566
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8059..8292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8477..8521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
       61 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt
      121 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      181 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      241 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      301 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      361 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
      421 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
      481 tagaataact tcgtatagca tacattatac gaagttatga attcgccacc atgaagatta
      541 acggcgtcaa cttccctagc aacggccctg tgatgcagaa gaagaccctg ggatgggagg
      601 caagcacaga aatgctctac ccagctgata gcggactccg ggggcactcc caaatggctc
      661 tcaagctggt ggggggaggc tacctgcact gcagcctgaa gaccacatat aggtctaaaa
      721 aaccagctaa gaatctgaag atgcccggct tctacttcgt cgatagaagg ctggagagga
      781 tcaaggaggc cgacaaggaa acctatgtgg aacagcacga gatggccgtg gctaggtact
      841 gcgatctccc ctccaagctc ggccattcca tggtgggcga agacagcgtg ctgattaccg
      901 aaaatatgca catgaaactg tacatggaag gcaccgtcaa cgaccaccac ttcaaatgca
      961 cctctgaggg ggagggcaag ccttatgaag gcactcagac catgaagatc aaggtcgtgg
     1021 aagggggacc tctcccattt gcattcgaca tattggccac tagcttcatg tatggctcca
     1081 agacatttat taaccacaca cagggcatcc ctgatttctt caaacagtct ttccctgaag
     1141 gattcacctg ggagcggatt accacctacg aggacggagg cgtgctgaca gccacccagg
     1201 acacatccct gcaaaacgga tgtctcatct ataacgtcgg agtgctgaca gctacccagg
     1261 atacctccct gcagaacggc tgtctgattt ataacgtcaa gatcaacggc gtcaacttcc
     1321 cctctaatgg gcctgtcatg cagaagaaga cactgggatg ataatagctc gagataactt
     1381 cgtatagcat acattatacg aagttatgga tcctctagca tgatcaacca tggtgggtga
     1441 ggatagcgtg ctgatcaccg agaacatgca catgaaactg tacatggagg gcaccgtgaa
     1501 cgaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     1561 catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     1621 cagcttcatg tacggcagca aaacctttat caaccacacc cagggcatcc ccgacttctt
     1681 taagcagtcc ttccctgagg gcttcacatg ggagaggatc accacatacg aagacggggg
     1741 cgtgctgacc gctacccagg acaccagcct ccagaacggc tgcctcatct acaacgtcaa
     1801 gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     1861 ggaggccagc accgagatgc tgtaccccgc tgacagcggc ctgagaggcc atagccagat
     1921 ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ctcaagacca catacagatc
     1981 caagaaaccc gctaagaacc tcaagatgcc cggcttctac ttcgtggaca ggagactgga
     2041 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgagatgg ctgtggccag
     2101 gtactgcgac ctgcctagca aactggggca cagctaagcg gccgcaattc taccgggtag
     2161 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     2221 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     2281 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     2341 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     2401 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     2461 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     2521 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     2581 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     2641 ctccgggcct ttcgacctgc agccaattgg ccaccatggc caagcctttg tctcaagaag
     2701 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     2761 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     2821 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     2881 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     2941 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     3001 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     3061 tgtgggaggg ctaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     3121 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     3181 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     3241 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     3301 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     3361 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     3421 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3481 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3541 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3601 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3661 cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3721 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg
     3781 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3961 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     4021 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4081 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4141 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tcyascyatc ccgcccctaa
     4201 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4261 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4321 gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     4381 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     4441 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     4501 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     4561 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     4621 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     4681 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     4741 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     5101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     5161 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     5221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     5281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     5341 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     5401 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     5461 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     5521 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5581 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5641 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     5701 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5761 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5821 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5881 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5941 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6001 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6061 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     6121 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     6181 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     6241 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     6301 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     6361 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     6421 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     6481 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     6541 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     6601 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     6661 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     6721 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6781 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     6841 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     6901 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     6961 acgacggcca gtgccaagct gacgcgtgta gtcttatgca atactcttgt agtcttgcaa
     7021 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     7081 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     7141 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     7201 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     7261 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     7321 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     7381 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7441 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7501 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7561 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7621 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7681 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7741 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7801 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7861 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccac tgatcttcag
     7921 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7981 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     8041 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     8101 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     8161 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     8221 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     8281 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     8341 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     8401 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8461 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8521 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8581 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8641 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     8701 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     8761 gaacggatct cgacggtatc ggt
//
