LOCUS       sequence-356280-        9247 bp    DNA     circular SYN 15-APR-2025
DEFINITION  lentiviral vector backbone for both barcodes and KTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9247)
  AUTHORS   Kudo T, Lane K, Covert MW
  TITLE     A multiplexed epitope barcoding strategy that enables dynamic 
            cellular phenotypic screens.
  JOURNAL   Cell Syst. 2022 May 18;13(5):376-387.e8. doi: 
            10.1016/j.cels.2022.02.006. Epub 2022 Mar 21.
  PUBMED    35316656
REFERENCE   2  (bases 1 to 9247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2022.02.006"; journalName: "Cell Syst"; date: 
            "2022-05-18- 18"; volume: "13"; issue: "5"; pages: "376-387.e8"
COMMENT     Sequence Label: pLenti EPICode screen backbone
FEATURES             Location/Qualifiers
     source          1..9247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4173..4197
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4203..4738
                     /label=mCMV promoter
                     /note="mouse cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    5196..5214
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     CDS             5260..5640
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(5719..5740)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     6327..6348
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6384..6408)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6439..7027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6492..6512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6910..6921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7078..7098)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7083..7412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7263..7398
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        7460..7507
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7526..7924
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             8005..8238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8310..8431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8347..8366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8401..8420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8450..8585
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8605..8624)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8606..8624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8634..8651)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8634..8650)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8643..8665)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8792..9220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8879..8898)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9089..9110
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttcg
     4201 agtggcacgt atactgagtc attagggact ttccaatggg ttttgcccag tacataaggt
     4261 caataggggt gaatcaacag gaaagtccca ttggagccaa gtacactgag tcaataggga
     4321 ctttccattg ggttttgccc agtacaaaag gtcaataggg ggtgagtcaa tgggtttttc
     4381 ccattattgg cacgtacata aggtcaatag gggtgagtca ttgggttttt ccagccaatt
     4441 taattaaaac gccatgtact ttcccaccat tgacgtcaat gggctattga aactaatgca
     4501 acgtgacctt taaacggtac tttcccatag ctgattaatg ggaaagtacc gttctcgagc
     4561 caatacacgt caatgggaag tgaaagggca gccaaaacgt aacaccgccc cggttttccc
     4621 ctggaaattc catattggca cgcattctat tggctgagct gcgttctacg tgggtataag
     4681 aggcgcgacc agcgtcggaa ccgtcgcagt cttcggtctg accaccgtag aacgcagagt
     4741 gatccaatgg tgggtcggaa cagcgccatc gccgccggtg tatgcggggc ccttttcatt
     4801 gggtactgca tctacttcga ccgcaaaaga cgaagtgacc ccaacttcaa gaacaggctt
     4861 cgagaacgaa gaaagaaaca gaagcttgcc aaggagagag ctgggctttc caagttacct
     4921 gaccttaaag atgctgaagc tgttcagaag ttcttccttg aagaaataca gcttggtgaa
     4981 gagttactag ctcaaggtga atatgagaag ggcgtagacc atctgacaaa tgcaattgct
     5041 gtgtgtggac agccacagca gttactgcag gtcttacagc aaactcttcc accaccagtg
     5101 ttccagatgc ttctgactaa gctcccaaca attagtcaga gaattgtaag tgctcagagc
     5161 ttggctgaag atgatgtgga atcgtcggca gcgtcagatg tgtataagag acagcagagt
     5221 gtcgggcgcg ccggatccag cgatcgcctc gagaccggta tgccagagcc agcgaagtct
     5281 gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc
     5341 aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag
     5401 caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg
     5461 aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc
     5521 tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg
     5581 gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaagtag
     5641 gccgcacata ggtaccgccc gggccctgca ggaatggtga gcaagggcga ggagctgttc
     5701 accggggttg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5761 gtccgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
     5821 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg
     5881 gcctgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5941 cccgaaggct acgtccagga gcgcaccatc tctttcaagg acgacggaac ctacaagacc
     6001 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     6061 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac
     6121 aacgtctata tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc
     6181 cacaacgttg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     6241 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gccatcagtc cgccctgagc
     6301 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgcctaa
     6361 gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggttg atatccagca
     6421 cagtggcggc cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     6481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     6541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     6601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     6661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     6721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     6781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     6841 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     6901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     6961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     7021 tccccgcctg gaattctgca gatatccggt tagtaatgag tttggaatta attctgtgga
     7081 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7141 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7201 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7261 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7321 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7381 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7441 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7501 atacgacaag gtgaggaact aaaccatggc caagcctttg tctcaagaag aatccaccct
     7561 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     7621 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     7681 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     7741 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     7801 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     7861 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     7921 ctaagcacaa ttcgagctct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7981 actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga agacaagatc
     8041 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8101 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8161 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8221 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     8281 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     8341 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     8461 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     8521 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     8581 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     8641 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     8701 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     8761 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     8821 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     8881 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     8941 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     9001 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     9061 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     9121 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     9181 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     9241 acaattt
//
