LOCUS       sequence-356284-       10495 bp    DNA     circular SYN 14-APR-2025
DEFINITION  encoding stblHIF under PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Kudo T, Zhao ML, Kovary K, LaGory L, Covert MW, Teruel MN
  TITLE     A dynamic HIF1-alpha-PPAR-gamma circuit controls a paradoxical 
            adipocyte regulatory landscape
  JOURNAL   bioRxiv (2022)
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            (2022)"
COMMENT     Sequence Label: stblHIF: pLenti-PGK-stblHIF-PGK-Puro
FEATURES             Location/Qualifiers
     source          1..10495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..44
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        207..311
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(312..380,381..1172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(530..549)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1343..1931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1832..1851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2085..2102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2219..2240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2255..2260,2261..2278,2279..2285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2293..2309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2298..2320
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2317..2333
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2317..2333
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2352..2372
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2354..2372
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2400..2626
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2627..2807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2854..2979
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3472..3705
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3890..3934
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4201..4318
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4364..4874
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4793..4811
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    4929..4953
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(7393..7404)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(7499..7523)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7554..8142
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7607..7627)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8025..8036)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8156..8655
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8191..8214)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8588..8607
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8676..9275
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8676..8695)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9172..9192
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9413..9646
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9718..9839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9755..9774)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9809..9828
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9879..10014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10034..10053)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10035..10053)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10063..10080)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10063..10079)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10072..10094)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(10221..10495,1..181)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10308..10327)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
       61 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
      121 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
      181 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      241 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      301 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      361 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      421 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      481 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      541 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      601 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      661 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      721 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      781 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      841 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      901 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      961 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1021 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1081 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1141 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1201 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1261 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1741 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1981 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2041 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2101 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2161 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2221 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2281 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2341 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2401 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2461 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2521 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2581 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2641 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2701 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2761 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2821 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2881 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2941 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     3001 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     3061 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     3121 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3181 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3241 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3301 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3361 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3421 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3481 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3541 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3601 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3661 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3721 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3781 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3841 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3901 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3961 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     4021 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     4081 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     4141 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4201 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4261 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttat
     4321 cgatcacgag actagcctcg agaagcttga tatcgaattc cacggggttg gggttgcgcc
     4381 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     4441 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     4501 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     4561 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     4621 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     4681 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     4741 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     4801 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     4861 acctctctcc ccagggggat ccaccggttc gtcgactagt ccagtgtggt ggaattctgc
     4921 agatatcaac aagtttgtac aaaaaagcag gctccaccat ggagggcgcc ggcggcgaga
     4981 acgagaagaa aaagatgagt tctgaacgtc gaaaagaaaa gtctagagat gcagcaagat
     5041 ctcggcgaag caaagagtct gaagtttttt atgagcttgc tcatcagttg ccacttcccc
     5101 acaatgtgag ctcacatctt gataaagctt ctgttatgag gctcaccatc agttatttac
     5161 gtgtgagaaa acttctggat gccggtggtc tagacagtga agatgagatg aaggcacaga
     5221 tggactgttt ttatctgaaa gccctagatg gctttgtgat ggtgctaaca gatgacggcg
     5281 acatggttta catttctgat aacgtgaaca aatacatggg gttaactcag tttgaactaa
     5341 ctggacacag tgtgtttgat tttactcatt catgtgacca tgaggaaatg agagaaatgc
     5401 ttacacacag aaatggccca gtgagaaaag ggaaagaact aaacacacag cggagctttt
     5461 ttctcagaat gaagtgcacc ctaacaagcc gggggaggac gatgaacatc aagtcagcaa
     5521 cgtggaaggt gcttcactgc acgggccata ttcatgtcta tgataccaac agtaaccaac
     5581 ctcagtgtgg gtacaagaaa ccacccatga cgtgcttggt gctgatttgt gaacccattc
     5641 ctcatccgtc aaatattgaa attcctttag acagcaagac atttctcagt cgacacagcc
     5701 tcgatatgaa attttcttac tgtgatgaaa gaattactga gttgatgggt tatgagccgg
     5761 aagaactttt gggccgctca atttatgaat attatcatgc tttggattct gatcatctga
     5821 ccaaaactca ccatgatatg tttactaaag gacaagtcac cacaggacag tacaggatgc
     5881 ttgccaaaag aggtggatat gtctgggttg aaactcaagc aactgtcata tataatacga
     5941 agaactccca gccacagtgc attgtgtgtg tgaattatgt tgtaagtggt attattcagc
     6001 acgacttgat tttctccctt caacaaacag aatctgtgct caaaccagtt gaatcttcag
     6061 ataagaagat gactcagctg ttcaccaaag ttgaatcaga ggctacaagc tgcctttttg
     6121 ataagcttaa gaaggagcct gatgctctca ctctgctggc tgcagctgcc ggcgacacca
     6181 tcatctctct ggattttggc agcgatgaca cagaaactga agatcaacaa cttgaagatg
     6241 ttccattata taatgatgta atgtttccct cttctaatga aaaattaaat ataaacctgg
     6301 caatgtctcc tttaccttca tcggaaactc caaagccact tcgaagtagt gctgatcctg
     6361 cactgaatca agaggttgca ttaaaattag aatcaagtcc agagtcactg ggactttctt
     6421 ttaccatgcc ccagattcaa gatcagccag caagtccttc tgatggaagc actagacaaa
     6481 gttcacctga gagacttctt caggaaaacg taaacactcc taacttttcc cagcctaaca
     6541 gtcccagtga atattgcttt gatgtggata gcgatatggt caatgtattc aagttggaac
     6601 tggtggaaaa actgtttgct gaagacacag aggcaaagaa tccattttca actcaggaca
     6661 ctgatttaga cttggagatg ctggctggct atatcccaat ggatgatgat ttccagttac
     6721 gttcctttga tcagttgtca ccattagaga gcaattctcc aagccctcca agtatgagca
     6781 cagttactgg gttccagcag acccagttac agaaacctac catcactgcc actgccacca
     6841 caactgccac cactgatgaa tcaaaaacag agacgaagga caataaagaa gatattaaaa
     6901 tactgattgc atctccatct tctacccaag tacctcaaga aacgaccact gctaaggcat
     6961 cagcatacag tggcactcac agtcggacag cctcaccaga cagagcagga aagagagtca
     7021 tagaacagac agacaaagct catccaagga gccttaacct gtctgccact ttgaatcaaa
     7081 gaaatactgt tcctgaggaa gaattaaacc caaagacaat agcttcgcag aatgctcaga
     7141 ggaagcgaaa aatggaacat gatggctccc tttttcaagc agcaggaatt ggaacattat
     7201 tgcagcaacc gggtgactgt gcacctacta tgtcactttc ctggaaacga gtgaaaggat
     7261 tcatatctag tgaacagaat ggaacggagc aaaagactat tattttaata ccctccgatt
     7321 tagcatgcag actgctgggg cagtcaatgg atgagagtgg attaccacag ctgaccagtt
     7381 acgattgtga agttgcagct cccatacaag gcagcagaaa cctactgcag ggtgaagaat
     7441 tactcagagc tttggatcaa gttaactaag cctaagcggc cgcactcgag atatctagac
     7501 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gacaatcaac
     7561 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7621 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7681 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7741 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7801 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7861 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7921 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7981 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     8041 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     8101 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta
     8161 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     8221 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     8281 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     8341 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     8401 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     8461 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     8521 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     8581 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     8641 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     8701 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     8761 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     8821 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     8881 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     8941 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     9001 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     9061 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     9121 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     9181 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     9241 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
     9301 cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg
     9361 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     9421 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     9481 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9541 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9601 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     9661 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     9721 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9781 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9841 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9901 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9961 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
    10021 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
    10081 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    10141 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    10201 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    10261 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    10321 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    10381 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    10441 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacg
//
