LOCUS       sequence-356397-        8908 bp    DNA     circular SYN 14-APR-2025
DEFINITION  The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting 
            new guide sequences..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Xu X, Chemparathy A, Zeng L, Kempton HR, Shang S, Nakamura M, Qi LS
  TITLE     Engineered miniature CRISPR-Cas system for mammalian genome 
            regulation and editing.
  JOURNAL   Mol Cell. 2021 Aug 26. pii: S1097-2765(21)00648-1. doi: 
            10.1016/j.molcel.2021.08.008.
  PUBMED    34480847
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Aug 26. pii: S1097-2765(21)00648-1. doi: 
            10.1016/j.molcel.2021.08.008."
COMMENT     Sequence Label: pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2;
            EF1a-...
FEATURES             Location/Qualifiers
     source          1..8908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..94)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        266..645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        647..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     800..820
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             863..1043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1090..1215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1712..1945
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2130..2174
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2472..2589
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2648..2896
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2648..2668
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2819..2838
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3161..3372
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          3373..4311
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4268..4288
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4346..4942
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4346..4365)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4842..4862
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4952..5005
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5018..5716
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(5736..5769)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5825..6413
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5878..5898)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6296..6307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6416..6432)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6417..6433)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6942..7122
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7184..7772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7264..7283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7943..8734,8735..8803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8566..8585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8804..8908)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      241 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      841 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      901 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      961 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1021 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1081 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1141 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1201 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1261 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1321 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1381 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1441 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1501 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
     1561 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1621 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1681 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1741 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1801 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1861 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1921 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1981 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2041 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2101 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2161 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2221 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2281 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2341 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2401 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2461 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2521 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2581 caaaattttc gggtttatta cagggacagc agagatccag tttggttagt accgggcccg
     2641 ctctagagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     2701 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     2761 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     2821 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     2881 gaaaggacga aacaccgggc ttcactgata aagtggagaa ccgcttcacc aaaagctgtc
     2941 ccttagggga ttagaacttg agtgaaggtg ggctgcttgc atcagcctaa tgtcgagaag
     3001 tgctttcttc ggaaagtaac cctcgaaaca aattcatttg aatgaaggaa tgcaacggag
     3061 acgattaatg cgtctccttt ttttaagctt gggccgctcg agggatccat taggcggccg
     3121 cgtggataac cgtattaccg ccatgcatgt gcccgtcagt gggcagagcg cacatcgccc
     3181 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     3241 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     3301 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     3361 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     3421 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     3481 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     3541 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     3601 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     3661 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     3721 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     3781 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     3841 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     3901 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     3961 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     4021 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     4081 gcgccgtcca ggcacctcga ttagttctcg cgcttttgga gtacgtcgtc tttaggttgg
     4141 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     4201 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     4261 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     4321 gctagcccac catgaccgac ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     4381 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4441 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4501 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4561 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4621 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4681 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtttctcc
     4741 agaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4801 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4861 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4921 catgacccgc aagcccggtg ccggatcggg agagggcaga ggaagtctgc taacatgcgg
     4981 tgacgtcgag gagaatcctg gcccaccggt cgccaccagc gagctgatta aggagaacat
     5041 gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga
     5101 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
     5161 ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt
     5221 catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac
     5281 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     5341 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa
     5401 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga ctctgtatcc
     5461 tgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca
     5521 tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat
     5581 gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac
     5641 ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg
     5701 gcacaagctt aattaagaat tcgtcgaggg acctaataac ttcgtatagc atacattata
     5761 cgaagttata catgtttaag ggttccggtt ccactaggta caattcgata tcaagcttat
     5821 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5881 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5941 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6001 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6061 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6121 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6181 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     6241 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     6301 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     6361 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     6421 ccgtcgacct cgatcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     6481 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag
     6541 tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6601 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc
     6661 ttgatctgtg gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag
     6721 ggccagggat cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     6781 aagagaaggt agaagaagcc aatgaaggag agaacacccg cttgttacac cctgtgagcc
     6841 tgcatgggat ggatgacccg gagagagaag tattagagtg gaggtttgac agccgcctag
     6901 catttcatca catggcccga gagctgcatc cggactgtac tgggtctctc tggttagacc
     6961 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     7021 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     7081 gatccctcag acccttttag tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc
     7141 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7201 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7261 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7321 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7381 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7441 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7501 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7561 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7621 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7681 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7741 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7801 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7861 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7921 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7981 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     8041 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     8101 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     8161 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     8221 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     8281 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8341 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8401 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8461 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8521 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8581 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8641 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8701 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8761 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8821 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8881 agaaaaataa acaaataggg gttccgcg
//
