LOCUS       sequence-356624-       14048 bp    DNA     circular SYN 14-APR-2025
DEFINITION  CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning 
            site).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Gervais NC, La Bella AA, Wensing LF, Sharma J, Acquaviva V, Best M, 
            Cadena Lopez RO, Fogal M, Uthayakumar D, Chavez A, Santiago-Tirado 
            F, Flores-Mireles AL, Shapiro RS
  TITLE     Development and applications of a CRISPR activation system for 
            facile genetic overexpression in Candida albicans.
  JOURNAL   G3 (Bethesda). 2022 Dec 1:jkac301. doi: 10.1093/g3journal/jkac301.
  PUBMED    36450451
REFERENCE   2  (bases 1 to 14048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Dec 1:jkac301. doi: 10.1093/g3journal/jkac301."
COMMENT     Sequence Label: pRS156
FEATURES             Location/Qualifiers
     source          1..14048
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(3225..3251,3252..3254,3255..5810,5811..5813,
                     5814..7328)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7341..7361
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7452..7601
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7626..7646
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8076..8432
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             8451..9020
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      complement(9933..10130)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(10731..11074)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     complement(12037..12056)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12038..12056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12063..12080)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12063..12079)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12072..12094)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(12308..12327)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     misc_RNA        complement(12364..12438)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     13890..13911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcgtaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtaccag agctattaag
     2221 atcaccagcc tcgtttataa taaacttagt ctaatatttc ttttggacca aaatagaaac
     2281 tgttctcagg tggcaaaaaa cagcttaaag tctgtgttaa aaaaagatct cggctatgcc
     2341 aatcaaaaag gagtgacatt attatgtaaa agaagaaatg ttgtaattcc gcgaaacaac
     2401 aattttaatg cttactgtgc cgccaccaat atcctatggc caaggggcct ttaaacatgc
     2461 cacggaaata tacagtcaaa gtaaacctac tatgtcttta gagccttcag gatcaattgt
     2521 ttttaaatga atgactgcaa aactagaaaa gtagcctctt acataagcaa gtcatttgta
     2581 caccgagagt ttagtaacca gtgaagcaag tatatctggc aaccctgaaa cttatctgtg
     2641 aattataaag ctcataagta aaaagtgtag aaaagaagat aaggcagacc aaatacaaat
     2701 ttgcaacagt acaacgaata tttcagcgtc aaaactagag aataataaag aaaacgatct
     2761 tttcaaaaag aaaaaacctt ttagttttcc tttgttgttg ttgtgggtgt gtgctattta
     2821 tattatatag tttactcata ataccataaa atattcggtt tgattaggtt attttaataa
     2881 gctaatttgt ttctaatcgt gtaatttatg ctgtgtatat taagtagtgt gtgcactgcc
     2941 caaaaatgtt tgttgtttat agtcggttaa agagaaaaaa gaaaaaaaga tccatacaca
     3001 cacgttaatt agttgttcaa cgtaatacac tcatattttg ttcttatttg ctttcggtcg
     3061 ctgttctcac caagatttat tgccaacgaa acaatttttt tttatatatt ttcagatttt
     3121 tctttttttc ctttcctttc cttttctaat tttcactcct ggttttcttt ctttcttaga
     3181 aacattatct cgatattaat attaaaaaaa tataatcatt caaaatggat aaaaaatact
     3241 ccataggttt ggccattgga accaattcag ttggatgggc tgtaatcact gatgaatata
     3301 aggttccttc taaaaagttc aaagttttag gaaatactga tagacactcc attaaaaaga
     3361 atttaattgg tgccttgttg tttgacagtg gtgaaacagc tgaagcaact cgtttgaaaa
     3421 gaacagctag aagaagatat accagaagaa agaacagaat ttgttacttg caagaaatct
     3481 tttctaatga aatggcaaag gttgatgatt ctttttttca tagattggaa gaaagtttct
     3541 tagttgaaga agataagaaa catgaaagac atccaatctt tggtaatatt gtagatgaag
     3601 tggcttacca cgaaaaatat cctacaattt atcatttgag aaaaaaatta gttgactcca
     3661 ctgataaagc tgacttaaga cttatatatt tggcattggc tcacatgatc aagttcagag
     3721 gtcacttctt aattgaaggt gatttgaatc cagataattc cgatgtcgac aagttgttta
     3781 ttcaattggt tcaaacttac aaccaacttt tcgaggaaaa tccaatcaat gcctcaggtg
     3841 tggatgctaa agctatttta tctgctagat tgtcaaaaag tagacgtttg gaaaatctta
     3901 ttgcccaatt gccaggtgaa aaaaaaaatg gattgtttgg aaacttaatt gcattatctt
     3961 tgggtttgac cccaaatttc aaatcaaact ttgatttggc cgaggatgct aaacttcaat
     4021 tgtctaagga tacatacgat gacgatttag ataatcttct tgcccaaatt ggtgatcaat
     4081 atgcagattt attcttggca gctaagaact tgagtgatgc aattttgtta agtgacattt
     4141 tgagagtcaa cacagaaatt acaaaagctc ctttatccgc ctctatgatt aaaagatatg
     4201 atgaacacca tcaagattta accttgttga aagctcttgt acgtcaacaa ttaccagaaa
     4261 agtataaaga aatattcttt gatcaatcta aaaatggtta cgctggttat attgatggtg
     4321 gtgctagtca agaagaattt tataagttta tcaagccaat tcttgaaaaa atggatggta
     4381 ctgaggaatt gttggttaag ttgaatcgag aggatttact tcgtaagcaa agaacatttg
     4441 ataacggttc tatccctcat caaatccact taggtgagtt acacgctatc ttgagacgtc
     4501 aggaagattt ttaccctttt ttgaaagaca atcgtgaaaa aattgaaaaa attttgactt
     4561 tcagaattcc ttattacgtc gggccattgg ctagaggtaa ttctagattt gcatggatga
     4621 ctcgtaaatc tgaagagact atcaccccat ggaattttga ggaagttgtt gataagggag
     4681 cctccgctca atcattcatc gagagaatga ccaatttcga caaaaacttg ccaaatgaga
     4741 aggtgttacc taaacattct ttgttatatg aatatttcac tgtttataat gaattaacaa
     4801 aagtcaaata cgtcactgaa ggtatgagaa aaccagcatt cttaagtggt gaacaaaaga
     4861 aagctatcgt tgacttgtta ttcaaaacta atcgtaaagt cactgtcaaa caacttaaag
     4921 aggactattt taagaaaata gagtgtttcg attccgttga aatcagtggg gttgaggata
     4981 gatttaatgc tagtttagga acttatcacg atttgttgaa aattatcaaa gacaaagatt
     5041 ttttggataa tgaagaaaac gaagatatct tggaggacat tgtattgact ttgactttgt
     5101 ttgaggatag agaaatgatc gaagaacgtt taaaaactta cgctcactta ttcgacgata
     5161 aagtcatgaa acaattaaaa agaagaagat ataccggttg gggaagatta tctagaaagt
     5221 tgattaatgg tattagagat aagcaaagtg gtaaaaccat cttagatttc cttaaaagtg
     5281 atggttttgc aaacagaaac tttatgcagt tgattcatga tgatagtttg acctttaagg
     5341 aagatattca aaaagctcag gtttctggac agggtgattc attgcatgaa catattgcca
     5401 acttagcagg gtctccagct atcaaaaagg gaattttaca aactgttaaa gttgttgatg
     5461 agttggtgaa agttatggga cgtcataagc ctgaaaatat agttatagaa atggctagag
     5521 aaaatcaaac aactcagaaa ggacaaaaga attctcgtga aagaatgaaa agaatagaag
     5581 aaggaattaa agagttgggt tctcaaatct taaaagaaca cccagtcgaa aacacccagt
     5641 tacagaacga gaagctttac ttgtactatt tgcaaaacgg gcgtgacatg tatgtagatc
     5701 aagagttgga tatcaataga ctttcagatt acgatgtcga tcacatagtt cctcagtcat
     5761 ttttaaaaga tgattccatt gacaacaaag ttttaacaag atctgataag gctagaggta
     5821 aatcagacaa tgtgccatca gaagaagtgg ttaagaaaat gaagaactat tggagacaat
     5881 tgttaaatgc aaagttgatt actcagagaa aatttgacaa cttaactaaa gctgaaagag
     5941 gaggattatc cgaattagat aaagctggat tcataaaacg tcagttggtt gaaacaagac
     6001 aaattactaa acatgtagcc caaattttag attcccgaat gaatactaaa tatgatgaaa
     6061 atgacaaatt aatcagagag gtcaaagtga tcacattaaa gtcaaagttg gtgtcagatt
     6121 tccgaaagga ttttcaattc tacaaggtga gagagatcaa caattatcat catgctcacg
     6181 acgcatattt gaatgcagtg gtaggaactg ccttaataaa gaagtatcct aagttagaat
     6241 ccgaattcgt gtatggtgat tataaagtct acgatgtcag aaagatgata gcaaaatcag
     6301 aacaagaaat tgggaaagcc accgcaaagt atttctttta ctccaacatc atgaatttct
     6361 ttaagaccga aatcaccttg gctaacgggg aaatcagaaa acgtcctttg attgaaacaa
     6421 atggagaaac cggtgaaatt gtgtgggata aggggagaga ttttgctact gttagaaaag
     6481 ttttatcaat gcctcaagtt aatattgtaa agaagactga agttcaaact ggtggtttct
     6541 ctaaggaatc cattcttcca aaacgaaact cagacaagtt gatcgcccga aaaaaagatt
     6601 gggatcctaa gaaatacgga ggttttgatt ctcctactgt cgcatattct gtcttggtcg
     6661 tggctaaagt tgaaaagggt aaatccaaaa aattgaaatc tgtgaaagaa ttgttaggta
     6721 ttaccatcat ggagcgttca tcctttgaga aaaacccaat tgactttctc gaagcgaagg
     6781 gctataaaga agtgaagaaa gatttgatta ttaagttgcc aaagtattct ctctttgaac
     6841 ttgaaaatgg gagaaagaga atgttggctt cagctggaga attgcaaaaa ggaaacgagt
     6901 tagctttgcc ttcaaagtac gttaatttct tatacttagc atctcattat gaaaagttga
     6961 aggggtcccc tgaagacaat gagcaaaagc aattattcgt agaacaacat aaacactatt
     7021 tggatgagat aattgaacaa atctccgagt tttctaaaag agttattttg gctgatgcaa
     7081 atttggataa agttttatct gcttataata agcatcgtga caaaccaatt cgagaacaag
     7141 ctgaaaatat tatccacttg ttcacattga ctaacttggg agctccagcc gcgtttaagt
     7201 actttgacac caccattgac cgtaagagat atacttcaac aaaagaagtg ttagacgcca
     7261 cattgattca tcagtccata actggtttat atgaaactag aatcgatttg tcacaattgg
     7321 ggggtgatag ccgagcggat cctaagaaaa aacgaaaagt ctccccaggt ataagaagat
     7381 tagacgctct tatatctacc agtctttata aaaaggccgg atacaaggag gcaagtggat
     7441 ctggtagagc agacgctttg gatgattttg atttagatat gttagggtca gacgcacttg
     7501 atgactttga tttagacatg ttggggtccg atgcacttga cgacttcgac ttggatatgc
     7561 ttgggtctga tgcccttgat gattttgacc ttgatatgtt aataaacagt cgatcatccg
     7621 ggtcaccaaa aaagaagaga aaggtgggtt cacaatactt acctgatact gatgacagac
     7681 accgaattga agaaaaacgt aagcgtactt atgagacatt taagagtata atgaagaaga
     7741 gtcctttttc agggccaact gacccaagac ctcctccacg aagaatcgcc gttcctagtc
     7801 gttcttcagc ttccgtgcca aagcctgctc ctcagccata ccctttcacc tcatctcttt
     7861 caacaatcaa ttacgacgaa tttccaacca tggtgtttcc ttctggacag atctcacaag
     7921 ctagtgcact tgcacctgct cctccacaag tacttcctca agcaccagca cctgccccag
     7981 cccctgcaat ggtgtctgca ttggctcaag ctccagcccc agtcccagtt ttagcccctg
     8041 gacctccaca ggccgttgcc ccaccagctc ctaagccaac ccaagccggt gaaggtactt
     8101 tgagtgaagc tttacttcag ttgcagtttg atgatgaaga tcttggtgca cttttaggta
     8161 actctactga tccagcagtt tttaccgact tagcatccgt cgataactct gagtttcaac
     8221 aattgcttaa tcagggaata ccagtagctc cacatactac tgagccaatg ttaatggagt
     8281 accctgaggc catcacacgt ttggtaactg gagcccaacg acctccagac cctgctcctg
     8341 ctccattagg agccccagga cttccaaatg gtttactttc cggagacgag gacttttctt
     8401 ccatcgctga catggatttc tctgctttat tggggtcagg ttctggaagt agagattcca
     8461 gagagggaat gtttttacct aaaccagaag caggaagtgc catctctgac gtcttcgaag
     8521 gtagagaggt atgccaacca aagcgtattc gacctttcca tccaccagga agtccatggg
     8581 ccaatcgacc tcttcctgcc tctttagccc ctactccaac cggaccagtt cacgagcctg
     8641 tagggtcctt aactcctgca ccagtgcctc agccattaga tcctgctcca gccgtaaccc
     8701 cagaagcaag tcaccttttg gaagacccag acgaggagac ttctcaggca gtcaaagctt
     8761 tacgtgagat ggctgatacc gtaattcctc agaaagagga agcagccatt tgtggacaaa
     8821 tggatctttc tcatcctcca cctcgagggc acttggatga gttgaccact actttggaat
     8881 caatgaccga agatttgaat cttgacagtc ctttaactcc agaacttaac gagattttag
     8941 ataccttctt gaatgatgag tgcttgcttc acgccatgca catatcaaca ggacttagta
     9001 tatttgatac atcattgttt tgacccgggg gatccgagtg aaattctgga aatctggaaa
     9061 tctggttttg tattcttgtt attcttcttt ttgttattac atatataact tgttactttt
     9121 ttaaaaaaat ctttgtttat tttataaata tataaaacta aatttaagaa aaagagaaaa
     9181 atgttttatt tgagagattg aaattttact tgaatttagc ttagctttta taaagtatta
     9241 ttatgtaaaa aaacaaaaca aatatacatt aaaaagttaa gactataaaa tagccaccca
     9301 aggcatttct atatcttgtt gttgttgttt tcatcttctg tatcagagga acttatttta
     9361 ttattttcgt cacgggtatt ttctcttgtt tgatgattca tcccattcat tccatcataa
     9421 aattctagaa ggaccacctt tgattgtaaa tagtaataat taccaccctt atttaattat
     9481 ttatttaact tatttattta tttattatac atatatacaa atctaataaa gtgaaaatct
     9541 cccccttcac atttataatt ggccagtttt tttcaaataa gcattccaac cagcatctct
     9601 atacctttta ccttcaatat ctggatctct tcctttacca aacaatcctc taccaacaat
     9661 gataatatca gttccagtgc taacaacttc atcaacagtt ctatattgtt gtcctaatcc
     9721 atcaccttta tcatctaatc caactccagg tgtcataata agccaatcaa atccttcttc
     9781 ttgtccaccc atatcacgtt gggcaataaa tccaataaca aattccttat cggatttagc
     9841 aatttcaaca gttttttgag aatattctcc atacgaattc gagctcgttt aaacgtcgac
     9901 actggatggc ggcgttagta tcgaatcgac agcagtatag cgaccagcat tcacatacga
     9961 ttgacgcatg atattacttt ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg
    10021 cgaaaaaaaa tataaatcac gctaacattt gattaaaata gaacaactac aatataaaaa
    10081 aactatacaa atgacaagtt cttgaaaaca agaatctttt tattgtcagt actgactcga
    10141 gttattatgg acatggcata gacatataca aagcttgttc accatcggaa gcagtaccat
    10201 cgtataaagc agtatccaaa ccacacaaag tgaaacccat tcttctataa gcatgaatag
    10261 ctggagcatt aacattggta acttccaacc acaaatgacc agcacctctt tctctggcga
    10321 attcagtagc caaacccatc aaagctctac caacaccatg acctctatgt tctggagcaa
    10381 cttcaatatc ttcaacagtc aatcttctgt tccaaccaga ataagaaaca acaacgaaac
    10441 cagccaaatc accatcatca ccataagcaa cgaaagttct agaatctgga tcaccatctt
    10501 caccagcatc ggattcatca tcggattcat catctgggaa aaccttagtc aatggtggat
    10561 caactggaac ttctctcaaa gtgaaaccat caccagtagc agtaactcta aaaacagtat
    10621 cggtagtgaa agaaccatcc aaagcttcaa tagcttcagc atcacctgga acagaagttc
    10681 tgtatctata agcagtatca tccaaagtag tagacataat tgtaggatcc ggttgtttat
    10741 gttcggatgt gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga
    10801 agaacctcag tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac
    10861 aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt
    10921 aatttttgct tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg
    10981 gatgtatggg ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa
    11041 gactgtcaag gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg
    11101 aggcaagctt gatatgctaa tgatcccact gatgataatt cagctaacat caataaccct
    11161 cttggctctt ggttggtggt ggtttcttta gctccctgtt ttaatccttc aactactcca
    11221 ttcccagtga caccatgagc attagtaata tctgcccaac tactaatttt ataaactcca
    11281 ccaatatatt gtttcttcac ggtattacca atatcagcaa attttctatc ttcaaaaatc
    11341 ataaattgat gtttacgtga aagttctaat aatggttcaa tagtggattc ataggaaaaa
    11401 tcattgatta tatcaatatg agtcttgatt aagcatacat aaggacccaa tttatcaatt
    11461 aattcaagga attccttagt ggtatcaaca tcaattgatg cacataaatt ggttttcttc
    11521 agttccatta atcgaaataa tcgttgtgct actggtgagg catgagtttc tgctctctca
    11581 ctataggtct tagtgttgac tgtcatatct tttgtttgct atttctattt ctatttcttt
    11641 cttcaactgt aaaaggggcg atatataaac cagtgtgtat ggggttgttg ctcaggaatt
    11701 gatcaatgtt taaaagaatg gttgaaatca tcattgaaga acaaaaaaaa agattttttc
    11761 tttcattatg ttatctgtat aatatgcagg aaataagatt gcaaaaaaat agccgttttt
    11821 ttggatacta tcaaacaaga ggaatgacta atacagaatt agtgttacga atcaatggca
    11881 ctacagcaac tttcaacaaa ataatctacg ttcaaaatat aatatatact attaaataaa
    11941 atcaaataat aaaataattt taaagaaagt agtaccagct cttttttttg tttccgttta
    12001 taccatccaa atcaattcct attagtctcc aattcgccct atagtgagtc gtattacaat
    12061 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    12121 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    12181 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
    12241 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
    12301 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg caaaaaaaag
    12361 caccaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
    12421 tatttctagc tctaaaactg aagagccatt aatgctcttc ctcgaaatta aaaatagttt
    12481 acgcaagtct cgaacttcca ccacaattta attgtaagaa cgctgcgaaa acatattctc
    12541 caatcctatt gggccgaagt ttcagctatg ccaaaggcat caaatttttt tttataaaat
    12601 tttccacttt tccataccag tgaaattatc acgtgatgga gtgagtatct ataatatttg
    12661 attgtttctt tgatgaggca atactcgagt atagttattg accaatgact tttaccggct
    12721 aaacaagtgg tattcaagca caattcagtt ttctggatct ctaataattg caattgcaat
    12781 tgcttcacat actctgttta taattcagtt tctttctctt gatgttactg atatattaag
    12841 ttaataaata ccaataactt tttttcaaaa atcttctatt ttcctttcaa ttgattattt
    12901 aaacacgaat cgttaataag ctgtgattgc tttttctctt tctttttctt ttctttgttc
    12961 aagtttgaaa aaaaaaaaaa aaggtttctc ttgttcgatg tagaattcag tgggttatag
    13021 ttgtaatgat atatggaata aaaagaaaga aagagaagag aaagagaaga agagaagaat
    13081 taagtaaacg tgtattagaa tttaaaaatt aaaattcatg ataatcttta ataataatta
    13141 actaagaatc acaaaattga aaatttgaaa gaaaaaagat cactccaagg tcgtgtcaag
    13201 tgactagaaa ttcttaatta aaatagttat aacaaaaaaa gaaatttgta tataacaatt
    13261 aagtaaaaga aaaaaaagca attcgctttg tataataata cccagaaaaa tatattcata
    13321 tattcataaa tcaaacatga aatgaagaat gctgaatcac ttgataggat ttagttccat
    13381 tatggtacta agaagtaata ttacttctat gaaaaggttg aatccgagat caaaattcat
    13441 cttttcatat actctggaga aaaaaaaaaa agtaattgta gtaagaatga caagtatcag
    13501 ttgagaaaaa aagtaacaaa gagaaagctc ggaggaggct ccccaaaggt tttatcacca
    13561 atggtggtac cactaaccca agaacagaaa aagcattgat attttgaaaa atacaatact
    13621 catcttttaa cccaaacctt ttcttgtcct ttaaaacttt tatctcatgt ctgtccaaag
    13681 tcatattgtc ttactacttt ctctccttca acgtccttcc ggctttcccc gtcaagctct
    13741 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    13801 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    13861 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    13921 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    13981 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
    14041 tacaattt
//
