LOCUS       sequence-356676-        9451 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Subcloned C7orf26v2 (ENST00000359073, Broad GPP TRCN0000477172) into
            pLX_HA backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Pan J, Kwon JJ, Talamas JA, Borah AA, Vazquez F, Boehm JS, Tsherniak
            A, Zitnik M, McFarland JM, Hahn WC
  TITLE     Sparse dictionary learning recovers pleiotropy from human cell 
            fitness screens.
  JOURNAL   Cell Syst. 2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005.
  PUBMED    35085500
REFERENCE   2  (bases 1 to 9451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2022 Jan 21. pii: S2405-4712(21)00488-9. doi: 
            10.1016/j.cels.2021.12.005."
COMMENT     Sequence Label: pLX_HA-C7orf26v2
FEATURES             Location/Qualifiers
     source          1..9451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..31)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     66..85
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      136..271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(291..310)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(292..310)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(320..337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(320..336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      478..933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(565..584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     775..796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        959..1063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1064..1132,1133..1924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1282..1301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2095..2683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2584..2603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2837..2854
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2971..2992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3007..3012,3013..3030,3031..3037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3045..3061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3050..3072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3069..3085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3069..3085
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3104..3124
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3106..3124
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3152..3378
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3379..3559
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3606..3731
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4224..4457
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4642..4686
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4917..5127
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        4939..5135
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4986..5121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5167..5769
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5167..5186)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5663..5683
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5812..5929
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6067..7245
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6298..7236
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7193..7213
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7265..7289
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8346..8370)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8378..8404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    8455..9043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8508..8528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8926..8937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9115..9348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    join(9426..9451,1..96)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
       61 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
      121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      241 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
      301 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
      481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
      541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
      601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
      661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
      721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
      781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
      841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
      901 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
      961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     1081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     1141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     1201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     1261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     1321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     1381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     1441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     2881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     2941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3001 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     3121 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     3181 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     3241 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     3301 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     3361 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3421 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3481 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3541 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     3601 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     3661 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     3721 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     3781 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     3841 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     3901 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     3961 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     4021 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     4081 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     4141 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     4201 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     4261 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     4321 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     4381 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     4441 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     4501 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     4561 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     4621 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     4681 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     4741 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     4801 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     4861 atgcattgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     4921 gcagaagtat gcaaagcatg cgtctcaatt agtcagcaac catagtcccg cccctaactc
     4981 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5041 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     5101 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ttctagaggt accaccggag
     5161 cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     5221 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     5281 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     5341 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg ccgtggcggt ctggaccacg
     5401 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     5461 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     5521 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     5581 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     5641 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     5701 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     5761 gccagttaac gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     5821 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     5881 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     5941 cagggacagc agagatccac tttggcgccg gctcgaggcc tgcaggtgca aagatggata
     6001 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     6061 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6121 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     6181 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6241 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     6301 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     6361 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     6421 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     6481 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     6541 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     6601 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     6661 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     6721 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     6781 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     6841 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     6901 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     6961 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     7021 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     7081 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     7141 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     7201 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggcta gcatcgattg
     7261 atcaacaagt ttgtacaaaa aagttggcat gagcgacatc cgccactcgc tgctgcgccg
     7321 cgatgcgctg agcgccgcca aggaggtgtt gtaccacctg gacatctact tcagcagcca
     7381 gctgcagagc gcgccgctgc ccatcgtgga caagggcccc gtggagctgc tggaggagtt
     7441 cgtgttccag gtgcccaagg agcgcagcgc gcagcccaag gagcaaacca aggactctgt
     7501 tcggcagatt attttttcat cccttttcag ccctcaaggg aacaaagccg atgacagccg
     7561 gatgagcttg ttgggaaaac tggtctccat ggcggtggct gtgtgtcgaa tcccggtgtt
     7621 ggagtgtgct gcctcctggc ttcagcggac gcccgtggtt tactgtgtga ggttagccaa
     7681 ggcccttgta gatgactact gctgtttggt gccgggatcc attcagacgc tgaagcagat
     7741 attcagtgcc agcccgagat tctgctgcca gttcatcacc tccgttaccg cgctctatga
     7801 cctgtcatca gatgacctca ttccacctat ggacttgctt gaaatgattg tcacctggat
     7861 ttttgaggac ccaagcgtcc tgcaagtgct catgacgctg cagctgcacc tgaccgagaa
     7921 gaatctgtat gggcgcctgg ggctgatcct cttcgaccac atggtcccgc tggtagagga
     7981 gatcaacagg ttggcggatg aactgaaccc cctcaacgcc tcccaggaga ttgagctctc
     8041 gctggaccgg ctggcgcagg ctctgcaggt ggccatggcc tcaggagctc tgctgtgcac
     8101 gagagatgac ctgagaacct tgtgctccag gctgccccat aataacctcc tccagctggt
     8161 gatctcgggt cccgtgcagc agtcgcctca cgccgcgctc cccccggggt tctaccccca
     8221 catccacacg cccccgctgg gctacggggc tgtcccggcc caccccgccg cccaccccgc
     8281 cctgcccacg caccccggcc acaccttcat ctccggcgtg acctttccct tcaggcccat
     8341 ccgctaccca actttcttgt acaaagtggt tgatatctac ccatacgatg ttccagatta
     8401 cgcttagtaa tgaactagta ccggttaagt cgacaatcaa cgcgttaagt cgacaatcaa
     8461 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8521 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8581 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8641 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8701 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8761 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8821 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8881 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8941 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9001 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     9061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9121 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     9361 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9421 agaggaactt gtttattgca gcttataatg g
//
