LOCUS       sequence-357018-         498 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged A1-LCD with thirty 
            Gly residues replaced with Ser, twelve Phe residues replaced with 
            Tyr (LCD: low-complexity domain, aa 186-320).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Bremer A, Farag M, Borcherds WM, Peran I, Martin EW, Pappu RV, 
            Mittag T
  TITLE     Deciphering how naturally occurring sequence features impact the 
            phase behaviours of disordered prion-like domains.
  JOURNAL   Nat Chem. 2022 Feb;14(2):196-207. doi: 10.1038/s41557-021-00840-w. 
            Epub 2021 Dec 20.
  PUBMED    34931046
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41557-021-00840-w"; journalName: "Nat Chem"; date: 
            "2022-02"; volume: "14"; issue: "2"; pages: "196-207"
COMMENT     Sequence Label: A1-LCD-30G+30S-12F+12Y
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaga acctgtattt tcagggtagc atggcaagcg caagcagcag ccagcgtagc
      121 cgtagcagca gcggtaatta tagcggtagc cgttcaggta gctatagcgg caatgataat
      181 tatggtcgca gtggcaatta tagtggtcgt agcggttatt caggtagtcg tagtggtagc
      241 ggctatagtg gttcaagcga tagctataat tcctatggta atgacagcag caattattct
      301 ggttccagca gctataatga ttatggcaac tataataacc agagcagcaa ctatggtccg
      361 atgaaaagtg gtaattattc cggtcgtagc agctcaagca gtggtagcag cggtcagtat
      421 tatgcaaaac cgcgtaatca gggttcatat agcggttcta gcagcagtag cagctattca
      481 agcagccgtc gttattaa
//
