LOCUS       sequence-357197-        4742 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor with homologous arms for Rab11b to knock in DExogron 
            (=minilAA7-DExCon) mCherry module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Gemperle J, Harrison TS, Flett C, Adamson AD, Caswell PT
  TITLE     On demand expression control of endogenous genes with DExCon, 
            DExogron and LUXon reveals differential dynamics of Rab11 family 
            members.
  JOURNAL   Elife. 2022 Jun 16;11. pii: 76651. doi: 10.7554/eLife.76651.
  PUBMED    35708998
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-16"; volume: "11. pii: 76651. doi"; pages: " 
            10.7554/eLife.76651"
COMMENT     Sequence Label: pJET-50-DExogron-mCherry-R11b
FEATURES             Location/Qualifiers
     source          1..4742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(68..859,860..928))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     691..710
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(929..1033)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1101..1119
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1425..1443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1493..1511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1824..1842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1860..1878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1896..1914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1932..1950
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1968..1986
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2004..2022
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2040..2058
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(join(3694..3700,3701..3718,3719..3724))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3739..3760
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3880..3897)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4051..4639)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4131..4150)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
       61 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
      121 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      181 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      241 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      301 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      361 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
      421 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      481 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      541 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      601 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      661 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
      721 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
      781 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
      841 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
      901 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
      961 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1021 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     1081 tcatgacatt aacctataaa aataggcgta tcacgaggcc gcccctgcag ccgaattata
     1141 ttatttttgc caaataattt ttaacaaaag ctctgaagtc ttcttcattt aaattcttag
     1201 atgatacttc atctggaaaa ttgtcccaat tagtagcatc acgctgtgag taagttctaa
     1261 accatttttt tattgttgta ttatctctaa tcttactact cgatgagttt tcggtattat
     1321 ctctattttt aacttggagc aggttccatt cattgttttt ttcatcatag tgaataaaat
     1381 caactgcttt aacacttgtg cctgaacacc atatccatcc ggcgtaatac gactcactat
     1441 agggagagcg gccgccagat cttccggatg gctcgagttt ttcagcaaga tataatacga
     1501 ctcactatag ggcagtcttc cgccccgccc tcttgagctc gcccctgata ggctggcgcg
     1561 tccgtcactt caaaaaggtc cgcattcctt ccgcctttct ccaggacacc gagggcgagg
     1621 agggtggtac caagcggcgc ccaccctcag agcactactt ccatctctga ttggcttcgc
     1681 tgggtgcccg tcgctactcc actcgccgct gataggccat tcaaccgccg ctctgcgcgg
     1741 cgccggctcc gcccccgtcg ggtgtttgtg gtggggctgc ggagtcgccg atcccgccgg
     1801 aagcgccagg acaaattctt tactccctat cagtgataga gaacgtatga agagtttact
     1861 ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata gagaacgtat
     1921 aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc ctatcagtga
     1981 tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat ccagtttact
     2041 ccctatcagt gatagagaac gtataagctt tgcttatgta aaccagggcg cctataaaag
     2101 agtgctgatt ttttgagtaa acttcaattc cacaacactt ttgtcttata ccaactttcc
     2161 gtaccacttc ctaccctcgt aaagatggga tcgggtggga ctagtacgcg tggcttctct
     2221 gagaccgtgg acctgatgct gaacctgcag tccaataagg agggctctgt ggatctgaag
     2281 aacgtgagcg ccgtgcctaa ggagaagacc acactgaagg acccatccaa gccccctgcc
     2341 aaggcacagg tggtgggatg gccacccgtg cggaactaca gaaagaatat gatgacccag
     2401 cagaagacaa gctccactag tggcatggtg tccaagggcg aagaggacaa catggccatc
     2461 atcaaagaat tcatgcggtt caaggtgcac atggaaggca gcgtgaacgg ccacgagttc
     2521 gagattgaag gcgaaggcga gggcagacct tacgagggaa cacagaccgc caagctgaaa
     2581 gtgaccaaag gcggccctct gccttttgcc tgggacattc tgagccctca gtttatgtac
     2641 ggcagcaagg cctacgtgaa gcaccccgcc gatattcccg actacctgaa gctgagcttc
     2701 cccgagggct tcaagtggga gagagtgatg aacttcgagg acggcggcgt ggtcaccgtg
     2761 actcaagata gctctctgca ggacggcgag ttcatctaca aagtgaagct gcggggcacc
     2821 aactttccct ctgatggccc cgtgatgcag aaaaagacca tgggctggga agccagcagc
     2881 gagagaatgt accctgaaga tggcgccctg aagggcgaga tcaagcagcg gctgaaactg
     2941 aaggatggcg gccactacga cgccgaagtg aaaaccacct acaaggccaa gaaacccgtg
     3001 cagctgcctg gcgcctacaa cgtgaacatc aagctggaca tcaccagcca caacgaggac
     3061 tacaccatcg tggaacagta cgagagagcc gaaggcagac acagcacagg cggaatggac
     3121 gagctgtaca agggaaccgg tgctggaagt ggtacccggg acgacgagta cgactaccta
     3181 ttcaaaggtg cggccggtgg ggcacagacg ggcgaagtcg tggcggcgag gcggcgggca
     3241 gacgggccaa ggccgcgcta tctttctaga agatctccta caatattctc agctgccatg
     3301 gaaaatcgat gttcttcttt tattctctca agattttcag gctgtatatt aaaacttata
     3361 ttaagaacta tgctaaccac ctcatcagga accgttgtag gtggcgtggg ttttcttggc
     3421 aatcgactct catgaaaact acgagctaaa tattcaatat gttcctcttg accaacttta
     3481 ttctgcattt tttttgaacg aggtttagag caagcttcag gaaactgaga caggaatttt
     3541 attaaaaatt taaattttga agaaagttca gggttaatag catccatttt ttgctttgca
     3601 agttcctcag cattcttaac aaaagacgtc tcttttgaca tgtttaaagt ttaaacctcc
     3661 tgtgtgaaat tattatccgc tcataattcc acacattata cgagccggaa gcataaagtg
     3721 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3781 aattgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3841 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3901 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3961 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4021 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4081 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4141 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4201 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4261 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4321 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4381 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4441 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4501 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4561 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4621 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4681 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4741 ga
//
