LOCUS       sequence-357490-       10077 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmids for production of LV-based TFactivity reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Abe H, Abe K
  TITLE     PCR-based profiling of transcription factor activity in vivo by a 
            virus-based reporter battery.
  JOURNAL   iScience. 2022 Feb 14;25(3):103927. doi: 10.1016/j.isci.2022.103927.
            eCollection 2022 Mar 18.
  PUBMED    35281741
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-02-14"; volume: "25(3):103927. doi"; pages: " 
            10.1016/j.isci.2022.103927. eCollection 2022 Mar 18"
COMMENT     Sequence Label: pLVTFrep1-creb
FEATURES             Location/Qualifiers
     source          1..10077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(108..127)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        299..678
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        680..878
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     833..853
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             896..1076
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1123..1248
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1741..1974
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2159..2203
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2477..2594
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2675..2794)
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     CDS             complement(2804..2824)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      3529..3538
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3565..3596)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      3827..3836
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3831..3840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4216..4235
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4727..4753
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4769..4795
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    4836..5424
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4889..4909)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5307..5318)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5943..6123
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6149..6166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6155..6379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6425..6853
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6512..6531)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6722..6743
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6862..6882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6867..7196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7047..7182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7109..7128
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7244..7291
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7310..7684
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7814..7935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7851..7870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7905..7924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7984..8000)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7984..8000)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7997..8019)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8008..8024
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8032..8038,8039..8056,8057..8062))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8077..8098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8215..8232)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8386..8974)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8466..8485)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9145..9936,9937..10005))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9768..9787
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10006..10077,1..33))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
       61 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
      121 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
      181 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
      241 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
      301 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      361 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      421 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      481 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      541 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      601 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      661 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      721 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      781 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      841 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
      901 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      961 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     1021 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     1081 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     1141 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     1201 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     1261 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     1321 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1381 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1441 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1501 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1561 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1621 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1681 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1741 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1801 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1861 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1921 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1981 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2041 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2101 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2161 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2221 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2281 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2341 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2401 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctacaaatgg
     2461 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2521 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2581 aaattcaaaa ttttcgggtt tattacaggg acagcagaaa ttcactttga attcacaaaa
     2641 gctgggtacc gggccccccc tcgagtctag attagacgtt gatcctggcg ctggcgcaag
     2701 cagcagggtg tctatccatg ccgctctcct gggcgcagct catgggcagg gttcctgcgg
     2761 cctgttcctc cacctctgga gggaagccgt gagaatttcc tactacctta cgcttcttct
     2821 ttggagccgt gtacagcttc accttcaggg ggttcacgtg ggcctcggcg tgctcctcca
     2881 gcttcacctt gaagcccttg gcggtctggc ccaggtcggt cctcacgatc tggtgctcca
     2941 ccacgtggct ggggggcatg gccacgggct tctcggtctt gtaggtggag tgcagctggc
     3001 acctctggta gccgccgccc tccagcagca ggaactgggt cacgtcgccc ctcagcacct
     3061 ccttggccac gctcatcttc tcggaggact tgtcccagcc gatggtctgc ttcttcatca
     3121 cggggccgtc ggcggggaag ttggtgccgt ggtagatgga gttgtggatg aagcagttgc
     3181 cctccaggcg gatgctccag ctggcggtgg ccacgccgcc gtcctcgaac aggaaggacc
     3241 tctcgtagga catgccgtcg gggaaggcct gcttgaagta gtcgggcatg tcggcggggt
     3301 acttggtgaa gcacctgttg ccgtactgga acacggtgga caggatgtcg aaggagaagg
     3361 gcaggggctt gcccttgggc ttgatgatca ccagctcgga ggtctgcttg ccctcgtagg
     3421 ggttgccggt gcccacgccc tcgatggtga actcgtggcc gttcacgctg ccctccatgc
     3481 ggtacttggt gcgcatctcc tgggcgatgc cctgcttgga gtaggacacc atggtggcga
     3541 ccggtacagt accggattgc caagctggaa gtcgagcttc cattatatac cctctagtgt
     3601 ctaagcttgg ccgccgaggc cagatctgtt ctcccattga cgtcaatggg gtgtctgaaa
     3661 gacgtcacag tatgacggcc atgggatctg gcagctgacg tcactgtctg gtgcaggccc
     3721 tcgacgctag tgattggatc ccccccaaag cccccggggc tgtaattgcg tccctctccc
     3781 gctagggggc agcagcgagc cgcccggggc ggcgcgaatt cccgcggccg ccatggcggc
     3841 cgggagcatg cgacgtcggg cccaatctgc agcacgtgcc gcggtggcgg ccgcccggta
     3901 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     3961 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     4021 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     4081 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     4141 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     4201 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgtcgcg ctgttctcct
     4261 cttcctcatc tccgggcctt tcgacctcac ggtgttagcg ctaccggtcg ccaccatgtc
     4321 gggacgcggc aagcagggag gcaaagctcg cgccaaagcc aagacccgct cttctcgtgc
     4381 cggtctccag ttccccgtgg gccgagtgca ccgactgctc cgcaagggca actatgctga
     4441 gcgggtcggg gccggcgcgc cggtgtacct ggcggcggtg ctggagtacc tgactgccga
     4501 gatcctggag ctggcgggca acgccgcccg cgacaacaag aagacccgca ttatcccgcg
     4561 ccacttgcag ctggccatcc gcaacgacga ggagctcaac aagctgctgg gcaaagtaac
     4621 catcgctcag ggtggtgtcc tgcccaacat ccaggctgtg ctactgccca agaagaccga
     4681 gagtcaccac aaggccaaag gcaaagggta cctaggaggc accatgtacc catacgatgt
     4741 tccagattac gctgctgtag gttcaggtta cccttacgac gtccctgact acgctgctag
     4801 ctctagaaat tcctcgaggg atcaagctta tcgataatca acctctggat tacaaaattt
     4861 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4921 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4981 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5041 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5101 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5161 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5221 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     5281 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5341 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5401 tctccctttg ggccgcctcc ccgcatcgat accgtcgaga cctagaaaaa catggagcaa
     5461 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg
     5521 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg
     5581 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5641 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     5701 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     5761 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     5821 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     5881 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     5941 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6001 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6061 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6121 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6361 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     6421 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6841 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     6901 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     6961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7021 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     7141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7201 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     7261 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
     7321 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
     7381 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
     7441 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
     7501 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
     7561 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
     7621 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
     7681 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     7741 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     7801 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7861 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7921 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
     7981 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     8041 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     8101 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     8161 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     8221 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8281 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8341 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8401 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8461 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8521 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8581 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8641 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8701 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8761 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8821 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8881 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8941 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     9001 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     9061 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     9121 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     9181 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     9241 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     9301 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     9361 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9421 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     9481 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9541 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9601 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9661 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9721 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     9781 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9841 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9901 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9961 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    10021 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatg
//
