LOCUS       sequence-357944-       14632 bp    DNA     circular SYN 15-APR-2025
DEFINITION  mammalian co-expression of human SEPT9_i3 NCmut fused to GFP10 and 
            of human SEPT9_i3 NCmut fused to GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14632)
  AUTHORS   Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, 
            Eroles M, Milanovic V, Omi S, Pedelacq JD, Iv F, Bouillard L, 
            Llewellyn A, Gomes M, Belhabib M, Kuzmic M, Verdier-Pinard P, Lee S,
            Badache A, Kumar S, Chandre C, Brasselet S, Rico F, Rossier O, 
            Koenderink GH, Wenger J, Cabantous S, Mavrakis M
  TITLE     Human septins organize as octamer-based filaments and mediate 
            actin-membrane anchoring in cells.
  JOURNAL   J Cell Biol. 2023 Mar 6;222(3):e202203016. doi: 
            10.1083/jcb.202203016. Epub 2022 Dec 23.
  PUBMED    36562751
REFERENCE   2  (bases 1 to 14632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-03-6"; volume: "222(3):e202203016. doi"; pages: 
            " 10.1083/jcb.202203016. Epub 2022 Dec 23"
COMMENT     Sequence Label: pTRIP TRE BI_SEPT9_i3 NCmut-14-GFP11_SEPT9_i3
            ...
FEATURES             Location/Qualifiers
     source          1..14632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    22..103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2051..2438
                     /label=bidirectional TRE promoter
                     /note="Tet-responsive bidirectional promoter PTight-BI, 
                     consisting of seven tet operator sequences flanked on each 
                     side by the minimal CMV promoter"
     protein_bind    2132..2150
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2168..2186
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2203..2221
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2239..2257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2275..2293
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2310..2328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2346..2364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(4457..4474)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4684..4703
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4865..5566
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             5849..6029
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7557..7576)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7790..7809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7909..7931
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7969..7987)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8055..8159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8160..8228,8229..9020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8378..8397)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9191..9779
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9680..9699
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9933..9950
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10020..10040)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10025..10354
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10205..10340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11488..11553
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11683..11703
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12128..12262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     12219..12238
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             12430..13063
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13110..13235
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    13728..13961
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             14146..14190
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    14483..14600
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttggctgata cgcgtacgtc ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
       61 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
      121 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
      181 ctacaaatgt ggtatggctg attatgatcc tctagattag gaggcgtcgg tgatgccggc
      241 ggcggtcacg tactccagca gcaccatgtg gtcgcgcttc tctccggagg acccaccacc
      301 tccagagcca ccgccaccat cgatcatctc tggggcttct ggctccttct cctccacgcc
      361 gttggccatg gcgctgctgc cctcgttgag gcgcttcaca cggtacgcct cgaagtggat
      421 gctgctggtg atgtccttga tgttctgcat gtgcgtcctg atgagaaggt cccgcaggta
      481 ggcaaactca cagtgtgtgg tgttttcaac ttcgatggta ccccacttgg tcttcctccc
      541 aaggatcctc ttgccgttga cctggtactc gtggtcactg cccaccacag caaatgggat
      601 catctcccgg aacttctcgt tcaccagccg gtcctccgag tcctcatcaa attccttctg
      661 ggggtacacg tcgatgccgt tggacagcag gtctgcggtg atccgctgtt tgaagtggac
      721 cctctcctcc agggtgagtg tgtccgcctt ggcgatgaca gggacgatgt tgaccacctt
      781 gctcaggcgt ttcataaact cgatgtccag gggcctgagg gagtggccgg tggcggggat
      841 gaagtagagg cagcagtgga cgcgggtgtc cgggatgcgc ttcttgcggt tgatgttgac
      901 ctcctcctgc aggtatttct cgtactggtc attgatgaac ttcatgatgg gctgccagca
      961 gttctcgttg ttgatgtggt ccccgaaccc tggtgtgtca atcactgtca gcttcatccg
     1021 gacgcctttc tcctcaatat cgtgcgtgat ggacttgatc tcgatggtct tggggatgcg
     1081 ctcctctgag gtgggctgca ccgacttccg gctgattttg gatttgaaga gggtgttgat
     1141 taaggtggat ttacccaagc cgctctgccc gaccaccatg atgttgaact cgaagccctg
     1201 cttcatggcc ttccggcgat cctgctccag gatggagtca tcccccacgt agccgaagtc
     1261 caccggggcc ttctcgttcc gtgatgcagg cgcctgcttg agcccggcat ctctgggggt
     1321 gtcggccatg tcgccaacgc agctgggagc cgcctcagtg gcctcctggc tccggggtgt
     1381 agcctcagcc acagggggct ggggcttggg ctccagcctg ctctgcagct gagacacagg
     1441 ggcaggctct gaattttcca aggtctgggc tgggctgggg gcggtgggcg cctcagcagg
     1501 cttgggcatc tggatctcca ccctcttggg ggctgcgtcg gtggcagggg cagtgggcac
     1561 ctcggggacc ttggaggcag ggggctccat cctccggtgg gctgactcct ggggtttgac
     1621 gatggtgatc tccgtcctcc gaggggccgg ttctggcgtc ttgtggccca acacctcggc
     1681 cctcttgagc ccgaaccggg acgggccgat ggccccggcg ttctccacct gcttggacga
     1741 gatgtcaatg gacagctcag tgcgccggga caccggctcg gccgccttgg ggcccgagag
     1801 ctccaccctc cgcagggacg ccttggggga gcgttggctt agggagtcca catggcgggc
     1861 cgagggttct gagttcttca cgcccaggtc ctggaatttc tgggtggagc tggccacagt
     1921 ggctcggagt aggggagtct ggaccctccg gggtggggtg gagttgggtg tctcgacctc
     1981 ctcgacctca aaagatcttt tcaaggctga gatccggtcc ctctccatca tatgaccggt
     2041 gaattctcca ggcgatctga cggttcacta aacgagctct gcttatatag gcctcccacc
     2101 gtacacgcca cctcgacata ctcgagttta ctccctatca gtgatagaga acgtatgtcg
     2161 agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc agtgatagag
     2221 aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta
     2281 tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac gtatgtcgag
     2341 tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct
     2401 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagatttcga gctcggtacc
     2461 gcggatggag agggaccgga tctcagcctt gaaaagatct tttgaggtcg aggaggtcga
     2521 gacacccaac tccaccccac cccggagggt ccagactccc ctactccgag ccactgtggc
     2581 cagctccacc cagaaattcc aggacctggg cgtgaagaac tcagaaccct cggcccgcca
     2641 tgtggactcc ctaagccaac gctcccccaa ggcgtccctg cggagggtgg agctctcggg
     2701 ccccaaggcg gccgagccgg tgtcccggcg cactgagctg tccattgaca tctcgtccaa
     2761 gcaggtggag aacgccgggg ccatcggccc gtcccggttc gggctcaaga gggccgaggt
     2821 gttgggccac aagacgccag aaccggcccc tcggaggacg gagatcacca tcgtcaaacc
     2881 ccaggagtca gcccaccgga ggatggagcc ccctgcctcc aaggtccccg aggtgcccac
     2941 tgcccctgcc accgacgcag cccccaagag ggtggagatc cagatgccca agcctgctga
     3001 ggcgcccacc gcccccagcc cagcccagac cttggaaaat tcagagcctg cccctgtgtc
     3061 tcagctgcag agcaggctgg agcccaagcc ccagccccct gtggctgagg ctacaccccg
     3121 gagccaggag gccactgagg cggctcccag ctgcgttggc gacatggccg acacccccag
     3181 agatgccggg ctcaagcagg cgcctgcatc acggaacgag aaggccccgg tggacttcgg
     3241 ctacgtgggg gatgactcca tcctggagca ggatcgccgg aaggccatga agcagggctt
     3301 cgagttcaac atcatggtgg tcgggcagag cggcttgggt aaatccacct taatcaacac
     3361 cctcttcaaa tccaaaatca gccggaagtc ggtgcagccc acctcagagg agcgcatccc
     3421 caagaccatc gagatcaagt ccatcacgca cgatattgag gagaaaggcg tccggatgaa
     3481 gctgacagtg attgacacac cagggttcgg ggaccacatc aacaacgaga actgctggca
     3541 gcccatcatg aagttcatca atgaccagta cgagaaatac ctgcaggagg aggtcaacat
     3601 caaccgcaag aagcgcatcc cggacacccg cgtccactgc tgcctctact tcatccccgc
     3661 caccggccac tccctcaggc ccctggacat cgagtttatg aaacgcctga gcaaggtggt
     3721 caacatcgtc cctgtcatcg ccaaggcgga cacactcacc ctggaggaga gggtccactt
     3781 caaacagcgg atcaccgcag acctgctgtc caacggcatc gacgtgtacc cccagaagga
     3841 atttgatgag gactcggagg accggctggt gaacgagaag ttccgggaga tgatcccatt
     3901 tgctgtggtg ggcagtgacc acgagtacca ggtcaacggc aagaggatcc ttgggaggaa
     3961 gaccaagtgg ggtaccatcg aagttgaaaa caccacacac tgtgagtttg cctacctgcg
     4021 ggaccttctc atcaggacgc acatgcagaa catcaaggac atcaccagca gcatccactt
     4081 cgaggcgtac cgtgtgaagc gcctcaacga gggcagcagc gccatggcca acggcgtgga
     4141 ggagaaggag ccagaagccc cagagatgat cgatggtggc ggtggctctg gaggtggtgg
     4201 gtcctccgga atgggcgacc tgcccgacga ccactacctg tccacccaga ccatcctgag
     4261 caaggacctg aactaagcta gctaatagtt ccgcccctct ccctcccccc taacgttact
     4321 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     4381 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     4441 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     4501 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     4561 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     4621 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     4681 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     4741 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     4801 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatgc
     4861 aagcatgagc gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt
     4921 ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca
     4981 gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc
     5041 tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt
     5101 cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg
     5161 gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt
     5221 caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg
     5281 ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga
     5341 catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag
     5401 atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact
     5461 ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc
     5521 cagatactgc gacctcccta gcaaactggg gcacaagctt aattaaggat ccagcggccg
     5581 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     5641 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     5701 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5761 aggggggact ggaagggcta attcactccc aacgaagaca aaatcgtcga gagatgctgc
     5821 atataagcag ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5881 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5941 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6001 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6061 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt ataatagatt
     6121 tagcaggaat tgaactagga gtggagcaca caggcaaagc tgcagaagta cttggaagaa
     6181 gccaccagag atactcacga ttctgcacat acctggctaa tcccagatcc taaggattac
     6241 attaagttta ctaacattta tataatgatt tatagtttaa agtataaact tatctaattt
     6301 actattctga cagatattaa ttaatcctca aatatcataa gagatgatta ctattatccc
     6361 catttaacac aagaggaaac tgagagggaa agatgttgaa gtaattttcc cacaattaca
     6421 gcatccgtta gttacgactc tatgatcttc tgacacaaat tccatttact cctcacccta
     6481 tgactcagtc gaatatatca aagttatgga cattatgcta agtaacaaat taccctttta
     6541 tatagtaaat actgagtaga ttgagagaag aaattgtttg caaacctgaa tagcttcaag
     6601 aagaagagaa gtgaggataa gaataacagt tgtcatttaa caagttttaa caagtaactt
     6661 ggttagaaag ggattcaaat gcataaagca agggataaat ttttctggca acaagactat
     6721 acaatataac cttaaatatg acttcaaata attgttggaa cttgataaaa ctaattaaat
     6781 attattgaag attatcaata ttataaatgt aatttacttt taaaaaggga acatagaaat
     6841 gtgtatcatt agagtagaaa acaatcctta ttatcacaat ttgtcaaaac aagtttgtta
     6901 ttaacacaag tagaatactg cattcaatta agttgactgc agattttgtg ttttgttaaa
     6961 attagaaaga gataacaaca atttgaatta ttgaaagtaa catgtaaata gttctacata
     7021 cgttcttttg acatcttgtt caatcattga tcgaagttct ttatcttgga agaatttgtt
     7081 ccaaagactc tgaaataagg aaaacaatct attatatagt ctcacacctt tgttttactt
     7141 ttagtgattt caatttaata atgtaaatgg ttaaaattta ttcttctctg agatcatttc
     7201 acattgcaga tagaaaacct gagactgggg taatttttat taaaatctaa tttaatctca
     7261 gaaacacatc tttattctaa catcaatttt tccagtttga tattatcata taaagtcagc
     7321 cttcctcatc tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa aaatattggg
     7381 aaaaaattaa aaatagcaat acaacaataa aaaaatacaa atcagaaaaa cagcacagta
     7441 taacaacttt atttagcatt tacaatctat taggtattat aagtaatcta gccagatcct
     7501 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     7561 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     7621 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     7681 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     7741 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     7801 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7861 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7921 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7981 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8161 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8221 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8281 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8341 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8401 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8461 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8521 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8581 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8641 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8701 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8761 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8821 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8881 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8941 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9001 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9661 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9721 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9781 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9841 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9901 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9961 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
    10021 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
    10081 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
    10141 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
    10201 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
    10261 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
    10321 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
    10381 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
    10441 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
    10501 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
    10561 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
    10621 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
    10681 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    10741 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    10801 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
    10861 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    10921 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
    10981 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
    11041 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
    11101 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    11161 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
    11221 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    11281 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
    11341 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
    11401 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    11461 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
    11521 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
    11581 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
    11641 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
    11701 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    11761 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    11821 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    11881 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    11941 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    12001 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    12061 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    12121 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12181 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12241 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    12301 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    12361 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    12421 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    12481 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
    12541 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    12601 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    12661 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    12721 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    12781 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    12841 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    12901 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    12961 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    13021 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    13081 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    13141 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    13201 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    13261 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    13321 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    13381 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    13441 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    13501 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    13561 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    13621 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    13681 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    13741 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    13801 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    13861 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    13921 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    13981 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    14041 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    14101 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    14161 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    14221 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    14281 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    14341 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    14401 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattcac
    14461 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    14521 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    14581 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ct
//
