LOCUS       sequence-358124-         976 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Chen Z, Ioris RM, Richardson S, Van Ess AN, Vendrell I, Kessler BM, 
            Buffa FM, Busino L, Clifford SC, Bullock AN, D'Angiolella V
  TITLE     Disease-associated KBTBD4 mutations in medulloblastoma elicit 
            neomorphic ubiquitylation activity to promote CoREST degradation.
  JOURNAL   Cell Death Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi:
            10.1038/s41418-022-00983-4.
  PUBMED    35379950
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Differ. 2022 Apr 4. pii: 10.1038/s41418-022-00983-4. doi: 
            10.1038/s41418-022-00983-4."
COMMENT     Sequence Label: pcDNA 3.1 KBTBD4 R313PRR
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             67..144
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKGDYKDDDDKIDYKDDDDK"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttatggatt acaaggatga cgacgataag ggagattaca aggatgacga cgataagatc
      121 gattacaagg atgacgacga taagggatcc atggaatcac cagaggagcc tggagcatcc
      181 atggatgaga actactttgt gaactacact ttcaaagatc ggtcacattc aggccgtgtg
      241 gctcaaggca tcatgaaact gtgtctagag gaggagctct ttgctgatgt caccatttcg
      301 gtggaaggcc gggagtttca gctccatcgg ctggtcctct cagctcagag ctgcttcttc
      361 cgatccatgt tcacttccaa cctgaaggag gcccacaacc gggtgattgt gctgcaggat
      421 gtcagcgagt ctgttttcca gctcctggtt gattatatct accatgggac tgtgaaactt
      481 cgagctgagg agttgcagga aatttatgag gtgtcagaca tgtatcagct gacatctctc
      541 tttgaggaat gctctcggtt tttggcccgc acagtgcaag tgggaaactg ccttcaggtg
      601 atgtggctgg cagatcggca cagtgatcct gagctctata cggctgccaa gcactgtgcc
      661 aagacccacc tggcccagct gcagaataca gaggaatttc tccacttgcc ccaccgctta
      721 ctcacagata tcatctcgga tggagttccg tgttctcaga acccaacata ggcaatagaa
      781 gcctggatca actttaataa agaggaaaga gaggcttttg cagagtcact caggacaagc
      841 ttgaaggaaa ttggggagaa tgtgcacatt tacctgattg ggaaagagtc atctcntacc
      901 cactcgttgg ctgtgtcctt gcactgtgca gaagatgact ccatcattgt aagtggccaa
      961 aacagtttgt gccacc
//
