LOCUS       sequence-358971-       13127 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13127)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, 
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced 
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
  PUBMED    21952217
REFERENCE   2  (bases 1 to 13127)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-09-27"; volume: "2:483. doi"; pages: " 
            10.1038/ncomms1494"
COMMENT     Sequence Label: pBbA5c-MevT(CO)-MBIS(CO, ispA)
FEATURES             Location/Qualifiers
     source          1..13127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1..6,7..24,25..31)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             3707..3718
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             7327..7338
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      9773..9844
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      9860..9887
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(9915..9932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10049..10594)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(10129..10148)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(10708..10802)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(10823..11482)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     11397..11416
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(11483..11585)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(11657..12739)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12701..12720
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(12740..12817)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     13023..13042
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tttacacttt atgcttccgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
       61 gaattcaaaa gatctaaagg aggccatcct ggccatgaag aactgtgtga ttgtttctgc
      121 ggtccgcacg gcgatcggca gctttaacgg ctctttagcg agcacctctg caatcgatct
      181 gggtgcgacg gtcattaagg ccgccattga acgcgccaaa atcgacagcc agcacgttga
      241 tgaggtgatc atgggcaatg tgttacaagc cggcctgggt caaaacccag cgcgtcaagc
      301 actgttaaaa tctggtctgg ccgagaccgt gtgtggcttc accgtcaata aggtttgcgg
      361 ctctggcctg aagagcgtgg ccctggcagc acaagcgatt caagccggtc aggcacaaag
      421 catcgttgcg ggtggcatgg agaacatgtc tctggcgccg tacttattag atgccaaagc
      481 ccgcagcggt tatcgcctgg gcgatggtca ggtgtacgac gtcatcttac gcgatggctt
      541 aatgtgcgcg acccacggtt accacatggg tattacggcc gaaaacgtgg cgaaagaata
      601 cggcattacg cgcgagatgc aggatgaatt agcactgcac tctcagcgca aagcagcagc
      661 cgcgatcgag tctggtgcgt ttacggcgga aatcgtgcca gttaacgtgg tcacgcgcaa
      721 gaagacgttc gttttcagcc aggacgagtt cccgaaggca aacagcaccg cggaggcctt
      781 aggtgcctta cgcccagcct ttgacaaagc gggcacggtc accgccggta atgcgagcgg
      841 catcaatgat ggtgcagcgg cactggtcat catggaagag agcgccgcat tagcagcggg
      901 tctgacccca ttagcgcgca ttaaatctta tgccagcggc ggcgtcccac cagccctgat
      961 gggcatgggt ccggtcccag ccacgcaaaa agccctgcaa ttagcgggcc tgcaactggc
     1021 cgacattgat ctgatcgagg cgaacgaggc gtttgcagcg cagttcctgg cggtgggtaa
     1081 gaatctgggc ttcgacagcg agaaagtcaa tgtgaacggt ggcgcgattg cgttaggcca
     1141 tccgattggt gcaagcggcg cacgcatctt agtgacgtta ctgcacgcca tgcaggcacg
     1201 cgacaagacc ttaggcctgg cgaccttatg tattggtggc ggtcaaggta tcgccatggt
     1261 gatcgaacgc ctgaactgat gaggatctag gaggaaagca aaatgaaact gagcaccaag
     1321 ctgtgctggt gtggcatcaa gggtcgcctg cgcccacaaa agcagcaaca gctgcacaac
     1381 acgaacctgc aaatgaccga gctgaaaaag cagaagacgg ccgagcaaaa gacccgcccg
     1441 cagaacgttg gcatcaaggg catccagatt tatatcccga cgcagtgtgt caaccaatct
     1501 gagctggaga aattcgatgg cgtcagccag ggtaagtaca ccatcggcct gggccagacc
     1561 aacatgagct tcgtgaacga ccgtgaggac atctattcta tgagcctgac ggtgctgtct
     1621 aagctgatca agagctacaa catcgacacg aataagatcg gtcgtctgga ggtgggtacg
     1681 gagacgctga ttgacaagag caaaagcgtg aagtctgtct taatgcagct gttcggcgag
     1741 aacacggatg tcgagggtat cgacaccctg aacgcgtgtt acggcggcac caacgcactg
     1801 ttcaatagcc tgaactggat tgagagcaac gcctgggatg gccgcgatgc gatcgtcgtg
     1861 tgcggcgata tcgccatcta tgacaagggt gcggcacgtc cgaccggcgg tgcaggcacc
     1921 gttgcgatgt ggattggccc ggacgcacca attgtcttcg attctgtccg cgcgtcttac
     1981 atggagcacg cctacgactt ttacaagccg gacttcacga gcgaataccc gtacgtggac
     2041 ggccacttct ctctgacctg ctatgtgaag gcgctggacc aggtttataa gtcttatagc
     2101 aaaaaggcga tttctaaggg cctggtcagc gacccggcag gcagcgacgc cctgaacgtg
     2161 ctgaagtatt tcgactacaa cgtgttccat gtcccgacct gcaaattagt gaccaaatct
     2221 tatggccgcc tgttatataa tgatttccgt gccaacccgc agctgttccc ggaggttgac
     2281 gccgagctgg cgacgcgtga ttacgacgag agcctgaccg acaagaacat cgagaagacc
     2341 ttcgtcaacg tcgcgaagcc gttccacaaa gagcgtgtgg cccaaagcct gatcgtcccg
     2401 accaacacgg gcaacatgta taccgcgtct gtctacgcgg cattcgcgag cctgctgaat
     2461 tacgtcggtt ctgacgacct gcagggcaag cgcgttggcc tgttcagcta cggtagcggc
     2521 ttagcggcca gcctgtatag ctgcaaaatt gtcggcgacg tccagcacat catcaaggag
     2581 ctggacatca ccaacaagct ggcgaagcgc atcaccgaga cgccgaaaga ttacgaggca
     2641 gcgatcgagt tacgcgagaa tgcgcatctg aagaagaact tcaagccgca aggtagcatc
     2701 gagcacctgc agagcggcgt ctactacctg acgaacattg acgacaagtt ccgccgttct
     2761 tatgacgtca aaaagtaact agtaggagga aaacatcatg gtgctgacga acaaaaccgt
     2821 cattagcggc agcaaggtga agtctctgag cagcgcccaa agctctagca gcggcccgtc
     2881 tagcagcagc gaggaggacg acagccgtga cattgagtct ctggacaaga agatccgccc
     2941 gctggaggag ttagaggccc tgctgagcag cggcaacacc aagcagctga agaacaagga
     3001 agttgcagcg ctggtgatcc acggtaagct gccactgtat gcgctggaaa agaaactggg
     3061 cgatacgacg cgtgcggtcg cggtgcgtcg caaagcctta agcatcttag cggaggcccc
     3121 ggtgttagcc agcgaccgcc tgccgtacaa gaactacgac tacgaccgcg tgtttggcgc
     3181 gtgctgcgag aatgtcattg gctacatgcc gttaccggtt ggtgtgatcg gcccgctggt
     3241 cattgatggc acgagctatc acattccaat ggcgaccacg gaaggttgct tagtcgccag
     3301 cgccatgcgt ggctgtaagg cgattaacgc cggcggtggc gcgacgaccg tgttaaccaa
     3361 ggatggtatg acgcgcggtc cggtcgtccg cttcccaacg ctgaagcgca gcggcgcgtg
     3421 taagatttgg ctggattctg aggagggcca aaacgcgatc aagaaagcct tcaactctac
     3481 gagccgtttc gcgcgtttac agcatatcca gacctgcctg gccggcgacc tgctgttcat
     3541 gcgcttccgc accaccacgg gcgatgcgat gggcatgaac atgatcagca agggcgtcga
     3601 atatagcctg aaacaaatgg tggaagaata tggctgggag gacatggagg ttgtctctgt
     3661 gagcggcaac tattgcaccg acaagaagcc ggcagccatt aactggattg agggtcgcgg
     3721 caaaagcgtc gtggcagaag cgaccatccc aggcgacgtg gtccgtaagg ttctgaagag
     3781 cgacgtcagc gccctggttg agttaaatat cgcgaaaaac ctggtcggca gcgcgatggc
     3841 gggcagcgtg ggtggcttta acgcacatgc agcgaatctg gttacggcgg ttttcttagc
     3901 cttaggtcag gacccagccc aaaatgtcga gagcagcaac tgcattacct taatgaaaga
     3961 ggttgacggt gacctgcgca tcagcgtttc tatgccgtct atcgaggtcg gcacgatcgg
     4021 cggcggcacc gttttagaac cgcaaggtgc gatgctggat ctgctgggcg tgcgcggccc
     4081 acatgcaacg gccccaggca ccaatgcccg ccaactggcc cgtatcgtgg cctgcgcggt
     4141 tctggcgggt gagctgagcc tgtgcgccgc attagccgcg ggccatttag ttcaatctca
     4201 catgacccac aaccgcaagc cggcagaacc aaccaagcca aataacctgg acgcaaccga
     4261 cattaaccgt ctgaaggatg gcagcgtcac gtgcattaaa agctgatgag gatctaggag
     4321 gaaataacca tgtctctgcc attcctgacg tctgcgccag gtaaggtgat catcttcggc
     4381 gagcactctg cggtgtacaa taagccggcc gtcgccgcct ctgtgtctgc gttacgcacc
     4441 tacctgctga tcagcgaatc ttctgcaccg gacacgatcg agctggactt tccggacatc
     4501 agcttcaacc acaagtggag catcaacgac ttcaacgcga tcacggagga ccaggtgaac
     4561 agccaaaagc tggccaaagc ccagcaagca accgacggtc tgtctcagga gctggtgtct
     4621 ctgctggacc cgctgttagc gcagttaagc gagagcttcc attaccacgc cgcgttctgc
     4681 ttcctgtaca tgttcgtttg cctgtgcccg cacgcaaaga acatcaagtt cagcctgaag
     4741 agcacgctgc cgattggcgc aggcttaggc tctagcgcat ctatcagcgt gagcctggcg
     4801 ctggcgatgg cctatctggg tggcctgatt ggcagcaacg acctggagaa actgagcgaa
     4861 aacgacaagc acatcgtgaa ccagtgggcc tttatcggcg agaagtgcat tcatggcacc
     4921 ccgagcggca ttgacaacgc agttgccacg tatggcaacg ccctgctgtt cgagaaagac
     4981 agccacaacg gcacgatcaa cacgaacaac ttcaagttcc tggacgactt cccggcgatc
     5041 ccgatgattc tgacctacac ccgtatccca cgcagcacca aggatttagt cgcccgcgtg
     5101 cgtgttttag tcaccgaaaa gttcccggag gtgatgaagc cgatcctgga cgcgatgggc
     5161 gagtgcgcgc tgcagggtct ggagatcatg accaagctga gcaagtgcaa gggcaccgac
     5221 gatgaggcgg tggagaccaa caatgagctg tacgagcagc tgctggagct gatccgtatc
     5281 aatcacggcc tgctggtctc tatcggtgtg tctcacccgg gcctggaact gatcaaaaac
     5341 ctgagcgacg acctgcgcat tggctctacg aaattaacgg gtgcaggtgg cggtggctgc
     5401 tctttaacgc tgctgcgcag tgacattacg caggagcaaa tcgacagctt caagaagaag
     5461 ctgcaggacg acttcagcta cgagacgttc gagacggacc tgggcggcac gggctgttgc
     5521 ctgctgagcg ccaaaaatct gaacaaggac ctgaagatca aaagcctggt gttccagctg
     5581 ttcgaaaaca agacgaccac gaagcagcag atcgacgacc tgttactgcc gggtaacacc
     5641 aatctgccgt ggacgtcttg aggatctagg agggagatca tatgagcgaa ttacgtgcat
     5701 tcagcgcgcc aggtaaggca ctgctggccg gtggctacct ggtgttagac accaagtacg
     5761 aggcgttcgt cgtcggctta tctgcccgta tgcatgcagt tgcccacccg tatggtagcc
     5821 tgcagggctc tgacaagttc gaagtgcgtg tgaagagcaa gcagttcaag gacggcgagt
     5881 ggctgtacca cattagccca aagagcggct tcatcccggt tagcattggt ggcagcaaga
     5941 acccatttat cgagaaggtc attgccaacg tcttcagcta cttcaagccg aatatggacg
     6001 attactgcaa ccgcaacctg ttcgtcatcg acattttcag cgacgacgcg taccacagcc
     6061 aagaggactc tgttacggag catcgtggta accgccgcct gagcttccac agccatcgca
     6121 ttgaggaggt gccgaagacg ggtctgggtt ctagcgccgg tttagttacc gtcttaacga
     6181 cggcgttagc gagcttcttc gtgagcgacc tggagaacaa cgtggacaag taccgcgaag
     6241 tgattcataa cctggcgcag gtggcacatt gtcaggccca aggtaagatt ggctctggtt
     6301 ttgatgtggc agcggccgcc tatggctcta tccgctatcg ccgctttccg ccggccctga
     6361 tcagcaatct gccggacatc ggctctgcga cgtatggtag caaactggcg catctggtgg
     6421 acgaggagga ctggaacatc accattaagt ctaatcacct gccgagcggc ttaacgttat
     6481 ggatgggcga tatcaagaac ggcagcgaaa cggttaagct ggtgcagaaa gtgaaaaact
     6541 ggtacgacag ccacatgccg gaaagcctga agatttacac ggagctggac cacgccaata
     6601 gccgtttcat ggatggtctg agcaagctgg accgcctgca cgaaacccac gacgactaca
     6661 gcgaccaaat cttcgagagc ctggagcgca atgactgcac ctgccagaag tacccggaga
     6721 tcacggaggt ccgcgatgcc gtggcaacga ttcgccgtag cttccgcaaa attacgaagg
     6781 agagcggcgc ggatatcgaa ccaccggtcc agacgtctct gctggacgac tgtcaaacct
     6841 taaagggcgt gttaacgtgc ctgattccgg gcgcgggtgg ttacgacgcc attgccgtca
     6901 tcacgaaaca ggacgtcgat ctgcgcgcac aaacggccaa cgacaaacgt ttcagcaaag
     6961 tccaatggct ggatgttacg caggccgact ggggtgttcg caaggagaag gacccggaaa
     7021 cgtatctgga taagtgagga tctaggagga ttatgagatg accgtttaca cagcatccgt
     7081 taccgcaccc gtcaacatcg caacccttaa gtattggggg aaaagggaca cgaagttgaa
     7141 tctgcccacc aattcgtcca tatcagtgac tttatcgcaa gatgacctca gaacgttgac
     7201 ctctgcggct actgcacctg agtttgaacg cgacactttg tggttaaatg gagaaccaca
     7261 cagcatcgac aatgaaagaa ctcaaaattg tctgcgcgac ctacgccaat taagaaagga
     7321 aatggaatcg aaggacgcct cattgcccac attatctcaa tggaaactcc acattgtctc
     7381 cgaaaataac tttcctacag cagctggttt agcttcctcc gctgctggct ttgctgcatt
     7441 ggtctctgca attgctaagt tataccaatt accacagtca acttcagaaa tatctagaat
     7501 agcaagaaag gggtctggtt cagcttgtag atcgttgttt ggcggatacg tggcctggga
     7561 aatgggaaaa gctgaagatg gtcatgattc catggcagta caaatcgcag acagctctga
     7621 ctggcctcag atgaaagctt gtgtcctagt tgtcagcgat attaaaaagg atgtgagttc
     7681 cactcagggt atgcaattga ccgtggcaac ctccgaacta tttaaagaaa gaattgaaca
     7741 tgtcgtacca aagagatttg aagtcatgcg taaagccatt gttgaaaaag atttcgccac
     7801 ctttgcaaag gaaacaatga tggattccaa ctctttccat gccacatgtt tggactcttt
     7861 ccctccaata ttctacatga atgacacttc caagcgtatc atcagttggt gccacaccat
     7921 taatcagttt tacggagaaa caatcgttgc atacacgttt gatgcaggtc caaatgctgt
     7981 gttgtactac ttagctgaaa atgagtcgaa actctttgca tttatctata aattgtttgg
     8041 ctctgttcct ggatgggaca agaaatttac tactgagcag cttgaggctt tcaaccatca
     8101 atttgaatca tctaacttta ctgcacgtga attggatctt gagttgcaaa aggatgttgc
     8161 cagagtgatt ttaactcaag tcggttcagg cccacaagaa acaaacgaat ctttgattga
     8221 cgcaaagact ggtctaccaa aggaataagg atctaggagg taatgataat gcaaacggaa
     8281 cacgtcattt tattgaatgc acagggagtt cccacgggta cgctggaaaa gtatgccgca
     8341 cacacggcag acacccgctt acatctcgcg ttctccagtt ggctgtttaa tgccaaagga
     8401 caattattag ttacccgccg cgcactgagc aaaaaagcat ggcctggcgt gtggactaac
     8461 tcggtttgtg ggcacccaca actgggagaa agcaacgaag acgcagtgat ccgccgttgc
     8521 cgttatgagc ttggcgtgga aattacgcct cctgaatcta tctatcctga ctttcgctac
     8581 cgcgccaccg atccgagtgg cattgtggaa aatgaagtgt gtccggtatt tgccgcacgc
     8641 accactagtg cgttacagat caatgatgat gaagtgatgg attatcaatg gtgtgattta
     8701 gcagatgtat tacacggtat tgatgccacg ccgtgggcgt tcagtccgtg gatggtgatg
     8761 caggcgacaa atcgcgaagc cagaaaacga ttatctgcat ttacccagct taaataagga
     8821 tctaggaggt tactcatatg gactttccgc agcaactcga agcctgcgtt aagcaggcca
     8881 accaggcgct gagccgtttt atcgccccac tgccctttca gaacactccc gtggtcgaaa
     8941 ccatgcagta tggcgcatta ttaggtggta agcgcctgcg acctttcctg gtttatgcca
     9001 ccggtcatat gttcggcgtt agcacaaaca cgctggacgc acccgctgcc gccgttgagt
     9061 gtatccacgc ttactcatta attcatgatg atttaccggc aatggatgat gacgatctgc
     9121 gtcgcggttt gccaacctgc catgtgaagt ttggcgaagc aaacgcgatt ctcgctggcg
     9181 acgctttaca aacgctggcg ttctcgattt taagcgatgc cgatatgccg gaagtgtcgg
     9241 accgcgacag aatttcgatg atttctgaac tggcgagcgc cagtggtatt gccggaatgt
     9301 gcggtggtca ggcattagat ttagacgcgg aaggcaaaca cgtacctctg gacgcgcttg
     9361 agcgtattca tcgtcataaa accggcgcat tgattcgcgc cgccgttcgc cttggtgcat
     9421 taagcgccgg agataaagga cgtcgtgctc tgccggtact cgacaagtat gcagagagca
     9481 tcggccttgc cttccaggtt caggatgaca tcctggatgt ggtgggagat actgcaacgt
     9541 tgggaaaacg ccagggtgcc gaccagcaac ttggtaaaag tacctaccct gcacttctgg
     9601 gccttgagca agcccggaag aaagcccggg atctgatcga cgatgcccgt cagtcgctga
     9661 aacaactggc tgaacagtca ctcgatacct cggcactgga agcgctagcg gactacatca
     9721 tccagcgtaa taaataatta ggatccaaac tcgagtaagg atctccaggc atcaaataaa
     9781 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     9841 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atacctaggg
     9901 atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     9961 gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat acttaacagg
    10021 gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct gacaagcatc
    10081 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
    10141 cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
    10201 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
    10261 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc
    10321 ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc accactggca
    10381 gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg gttaaggcta
    10441 aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc ggttcaaaga
    10501 gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt cgttttcaga
    10561 gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta atcagataaa
    10621 atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag tcagccccat
    10681 acgatataag ttgttactag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc
    10741 gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag
    10801 tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta
    10861 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
    10921 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
    10981 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
    11041 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
    11101 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
    11161 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
    11221 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
    11281 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
    11341 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
    11401 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
    11461 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
    11521 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
    11581 gatcaacgtc tcattttcgc cagatatcga cgtcggtgcc taatgagtga gctaacttac
    11641 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11701 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt
    11761 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga
    11821 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg
    11881 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt
    11941 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
    12001 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt
    12061 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc
    12121 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc
    12181 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg
    12241 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa
    12301 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
    12361 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac
    12421 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg
    12481 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
    12541 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
    12601 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
    12661 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
    12721 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
    12781 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
    12841 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
    12901 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
    12961 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
    13021 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
    13081 acgatgcgtc cggcgtagag gatcgagatc gtttaggcac cccaggc
//
