LOCUS       sequence-35907-e         818 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Young-Pearse TL, Chen AC, Chang R, Marquez C, Selkoe DJ
  TITLE     Secreted APP regulates the function of full-length APP in neurite 
            outgrowth through interaction with integrin beta1.
  JOURNAL   Neural Dev. 2008 . 3():15.
  PUBMED    18573216
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural Dev.
            2008 . 3():15."
COMMENT     Sequence Label: pENTR APLP2 shRNA 3
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        150..398
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     150..170
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     321..340
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(455..554)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(571..590)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(572..590)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(595..611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(595..611)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(713..732)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(791..810)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg atgagcaatg
       61 cttttttata atgccaactt tgtacaaaaa agcaggcttt aaaggaacca attcagtcga
      121 ctggatccgg taccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
      181 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
      241 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
      301 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
      361 tcttggcttt atatatcttg tggaaaggac gaaacaccgc agatatggac cagtttaccc
      421 gaaggtaaac tggtccatat ctgctttttt ctagacccag ctttcttgta caaagttggc
      481 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      541 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
      601 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
      661 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
      721 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
      781 gatttatatg ggtataaatg ggctcgcgat aatgtcgg
//
