LOCUS       sequence-359565-       10024 bp    DNA     circular SYN 14-APR-2025
DEFINITION  expresses DSG-3 TSmod.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Jin X, Rosenbohm J, Moghaddam AO, Kim E, Seiffert-Sinha K, Leiker M,
            Zhai H, Baddam SR, Minnick G, Huo Y, Safa BT, Wahl JK 3rd, Meng F, 
            Huang C, Lim JY, Conway DE, Sinha AA, Yang R
  TITLE     Desmosomal Cadherin Tension Loss in Pemphigus Vulgaris Mediated by 
            the Inhibition of Active RhoA at Cell-Cell Adhesions.
  JOURNAL   bioRxiv [Preprint]. 2024 May 6:2024.05.03.592394. doi: 
            10.1101/2024.05.03.592394.
  PUBMED    38766211
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 May 6:2024.05.03.592394. doi: 
            10.1101/2024.05.03.592394."
COMMENT     Sequence Label: pcdna 3.1 DSG-3 Tension Sensor
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(68..87)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        259..638
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        639..842
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     793..813
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     887..906
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        887..905
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3202..3909
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(4030..4032,4033..4035,4036..4746)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4075..4096)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4336..4355)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4683..4704
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5557..5580
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5639..5656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5645..5869
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5915..6343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6002..6021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6212..6233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6352..6372)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6357..6686
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6537..6672
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6599..6618
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6753..7547
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6807..6826)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7417..7436
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7721..7842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7758..7777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7812..7831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7891..7907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7891..7907)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7904..7926)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7915..7931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7939..7945,7946..7963,7964..7969))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7984..8005
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8122..8139)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8293..8881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8373..8392)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9052..9843,9844..9912))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9675..9694
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9913..10017)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
       61 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
      121 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
      181 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
      241 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      301 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      361 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      421 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      481 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      541 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      601 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      661 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      721 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      781 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      841 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
      901 atagggagac ccaagctggc tagcgtttaa acgggccctc tagactcgag cggccgccac
      961 tgtgctggat atctgcagaa ttcactagtg atttattcac cagacacacc agcaacaatg
     1021 acctgcctct tccctagagc cttggggtct ctggccctcc tgatggtggt gctgctggta
     1081 cagggggaac tgcatgtgaa gccgggagga cagcacagag aagatgggac agcattgcag
     1141 ctggccaaaa gaaggtacaa acgtgaatgg gtgaaatttg caaaaccctg cagagaaaga
     1201 gaagacaact caagacgaaa cccaattgcc aagatcactt cagatttcca aaaaaaccag
     1261 aaaattacct accgcatttc tggagtggga attgatcagc ctccctttgg gatctttgtt
     1321 gttgacccaa acaatggtga tatcaacata acagccatag ttgatcgtga ggaaacccca
     1381 agcttcctga tcacatgccg tgcactaaat gccctgggac aggatgtaga gagaccactt
     1441 atattaacag tgaaaatttt agacgtcaat gataaccctc caatattttc acaaaccata
     1501 ttcaagggtg aaattgaaga aaatagtgct tcaaattccc tggtaatgat cctaaatgcc
     1561 acggatgcag atgaaccaaa ccatatgaac tctaaaattg ccttcaaaat tgtctcccag
     1621 gagcccgcag gcatgtccat gttcctcatc agcagaaaca ccggagaagt ccgcactttg
     1681 accagttctc ttgatcgaga gcaaattagt agctaccatc tggttgtgag cggtgcagac
     1741 aacgatggaa caggactgtc aactcagtgt gaatgtagta ttaaaattaa agatgtcaat
     1801 gacaacttcc cagtgctcag agagtctcag tattcagcac gtattgaaga aaatactttg
     1861 aatgcagagc tactccgatt tcaagtgaca gactgggatg aagaatacac agataattgg
     1921 ttggctgtgt atttcttcac ctctgggaat gaggggaatt ggtttgaaat tgaaacagat
     1981 cctagaacca atgaaggcat cctgaaggtg gttaaggctc tggattatga acaagtgcag
     2041 agtatgcagt ttagtattgc tgtcagaaac aaagctgagt tccaccagtc agtgatctct
     2101 cagtaccgag tgcagtcaac gccagtcaca attcaagtca ttgatgtccg agagggaatc
     2161 tcattccgtc ctccttccaa gacatttact gtgcaaaggg gtgtaagcac taacaaatta
     2221 gttggttaca tcctggggac ataccaagct actgatgagg acacgggtaa agctgcctcc
     2281 tctgtcagat atgtcctggg tcgtaatgat ggtggtttgc ttgttattga ttcaaagact
     2341 gctcagatca aatttgtcaa aaacatcgac cgagattcta ctttcatagt taacaagaca
     2401 atttctgctg aggttctggc catagacgaa aacactggta agacctccac aggcacaata
     2461 tatgtggaag tgcccagttt taatgaaaac tgcccgtcag ttgtcctcga gaagaaagac
     2521 atctgtactt cctcaccttc tgtgaccctc tcagtgagaa ccctggacag gggtaaatac
     2581 actggcccct acacagtgtc cctggaggag cagccactga agctgccagt catgtggacc
     2641 atcacgacgc tgaatgctac atcagctctt ctacaagccc agcagcaagt gtctccagga
     2701 gtgtacaatg tcccagtcat cgtgaaggac aatcaggatg ggctgtgtga cacaccagag
     2761 agcttgaccc tgacagtgtg tcaatgtgat gaccggagca tgtgcagagc ccctatcccg
     2821 agcagagagc ctaacacata tggggagtca tcctggaggc tggggcctgc tgccattggc
     2881 ttgatcctgc taggactcct gatgttgcta ttggccccac ttctgcttct gacctgtgac
     2941 tgtgggtcag gtcccattgg aggagcagcg acaggtggct ttatcccagt gcccgatggt
     3001 tcggaaggta caattcatca gtggggaatc gaaggagctc agcccgaaga caaggaaatc
     3061 acaaatattt gtgttcctcc agttacaacc aatggagccg attttatgga aagctctgaa
     3121 gtttgcacaa atacatatgc tggtgggacc atggtagaag gtgcttcagg aatggaaatg
     3181 attaccaagc tgggagtcga gatggtgagc aagggcgagg agaccacaat gggcgtaatc
     3241 aagcccgaca tgaagatcaa gctgaagatg gagggcaacg tgaatggcca cgccttcgtg
     3301 atcgagggcg agggcgaggg caagccctac gacggcacca acaccatcaa cctggaggtg
     3361 aaggagggag cccccctgcc cttctcctac gacattctga ccaccgcgtt cgcctacggc
     3421 aacagggcct tcaccaagta ccccgacgac atccccaact acttcaagca gtccttcccc
     3481 gagggctact cttgggagcg caccatgacc ttcgaggaca agggcatcgt gaaggtgaag
     3541 tccgacatct ccatggagga ggactccttc atctacgaga tacacctcaa gggcgagaac
     3601 ttccccccca acggccccgt gatgcagaag aagaccaccg gctgggacgc ctccaccgag
     3661 aggatgtacg tgcgcgacgg cgtgctgaag ggcgacgtca agcacaagct gctgctggag
     3721 ggcggcggcc accaccgcgt tgacttcaag accatctaca gggccaagaa ggcggtgaag
     3781 ctgcccgact atcactttgt ggaccaccgc atcgagatcc tgaaccacga caaggactac
     3841 aacaaggtga ccgtttacga gagcgccgtg gcccgcaact ccaccgacgg catggacgag
     3901 ctgtacaagg ggccaggtgg tgcagggcca ggtggtgcag ggccaggtgg tgcagggcca
     3961 ggtggtgcag ggcccggtgg tgcaggtcca ggtggtgcag gtccaggtgg tgcaggtcca
     4021 ggtggtgcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4081 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4141 gccacctacg gcaagctgac cctgaagctg atctgcacca ccggcaagct gcccgtgccc
     4201 tggcccaccc tcgtgaccac cctgggctac ggcctgcagt gcttcgcccg ctaccccgac
     4261 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4321 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4381 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4441 ctggggcaca agctggagta caactacaac agccacaacg tctatatcac cgccgacaag
     4501 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcggcgtg
     4561 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4621 gacaaccact acctgagcta ccagtccaaa ctgagcaaag accccaacga gaagcgcgat
     4681 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4741 tacaaggcgg ccgcgggagc tactggagct acagcggcac ttggtccctg ttccttgggt
     4801 tattcaggaa caatgagaac aaggcattcc actggaggga ccttgaagga ctatgctgcg
     4861 ccagtcaaca tgactttcct aggctcctat ttctctcaga aatcactcgc ctatgccgag
     4921 gaagaggatg agcgcgaagt gaacgactgt ttgctgatct acgatgatga aggcgaggat
     4981 gctgcccccc actcccccac cctcagctcc tgcagcattt ttgccgatga cctagacgac
     5041 aacttcttgg attcccttgg ccccaaattt aaaaagcttg cagagatctg tctcggtatt
     5101 gatgacgaag ccaagcaagc aaagccaggc cctaaagaca gtggttctgg ggctgatacc
     5161 tgcgctcgct ctatggaagt ccctcagtca ggttctaaca ggtaccagac tttgcctggc
     5221 agtctggaag tcacccaaac gggttctaaa atatgccaca cgctgtcagg caaccaagaa
     5281 acttctgtta tgtccacttc tgggtctgtc caccctgctg ttgccatccc tgaccctcta
     5341 cagcttggga actacttgct cacagaaact tactcaactt caggttcctt tgcacaaccc
     5401 accactgtta cctttgaccc ccatgtcaca caaaatgtca ctgtaacaga aagggtcatt
     5461 tgtccccttc ctagtgcctc cagcagtata gtggctccca cagaactgcg aggctcatac
     5521 aatatgctct atacaaaaga aacctgttcc catctagatt acaaggacga cgatgacaag
     5581 tagccgcggg cccgggatcc gagctcggta ccaagcttaa gtttaaaccg ctgatcagcc
     5641 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5701 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5761 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5821 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5881 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5941 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6001 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6061 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6121 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6181 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6241 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6301 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6361 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6421 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6481 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6541 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6601 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6661 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     6721 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6781 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6841 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     6901 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     6961 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7021 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7081 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7141 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7201 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7261 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7321 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7381 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7441 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7501 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     7561 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7621 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7681 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7741 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7801 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7861 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8701 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10021 attt
//
