LOCUS       sequence-360749-        2608 bp    DNA     circular SYN 15-APR-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-GA1
FEATURES             Location/Qualifiers
     source          1..2608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(336..341,342..358,359..364))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(491..1079)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(571..590)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1492..1508
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1492..1508
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1513..1532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1513..1531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1706..2608,1..288))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2525..2544
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag
       61 caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa
      121 ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg
      181 gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc
      241 cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg
      301 actgcgttag caatttaact gtgataaact accgcattaa agctaatcga tgataagctg
      361 tcaaacatga gaattaattc atgttctttc ctgcgttatc ccctgattct gtggataacg
      421 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      481 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      541 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      601 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      661 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      721 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      781 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      841 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      901 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      961 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1021 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1081 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgacgcg taactcacgt
     1141 taagggattt tggtcatggg tggctcgacc agatatggtc tcacctgatc tggggtgagt
     1201 ccgtgggctt gacagagaac tggaaggagg cgctgaaccg tctcaagagc ctgtttgcca
     1261 ccaatattac tagcaatcgc tacaacttgc tccggagtca gaccgtgggc ttgacagaga
     1321 actggcagta ggcgctgtac ggtttcaaga gcctgtttgc caccattgtt actagcgatg
     1381 gccaccacct gctccggggc gagaccagct ttggtctaga ggaggcatga tgatatattt
     1441 ttatcttgtg caatgtaaca tcagagattt tgagacacgg gccagagctg ccaggaaaca
     1501 gctatgacca tgtaatacga ctcactatag gggatatttt attttctccc aatcaggctt
     1561 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatca agggttggtt
     1621 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     1681 ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac
     1741 gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc
     1801 atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccaatg atcgaagtta
     1861 ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc
     1921 tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa
     1981 tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg
     2041 ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga
     2101 cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg
     2161 tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc
     2221 ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg
     2281 cccaccggaa ggagctgact gggttgaagg ctctcaaggg catgggtcgg cgctctccct
     2341 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
     2401 ccgcaaggaa tggtgcatgt aaggagatgg cgcccaacag tcccccggcc acggggcctg
     2461 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
     2521 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
     2581 ccacgatgcg tccggcgtag agaatcca
//
