LOCUS       sequence-361263-        2602 bp    DNA     circular SYN 15-APR-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-TG3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(395..414)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(396..414)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(419..435)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(419..435)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      842..1430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1331..1350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1557..1562,1563..1579,1580..1585)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1633..2602,1..221)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1985..2004)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta
       61 ccagcctaac ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga
      121 gcacatggaa cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg
      181 cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct
      241 cgctaacgga ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa
      301 tgcgcaaacc aacccttgat cggggaagaa cagtatgtcg agctattttt tgacttactg
      361 gggatcaagc ctgattggga gaaaataaaa tatcccctat agtgagtcgt attacatggt
      421 catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca
      481 agataaaaat atatcatcat gcctcctcta gaccaaagct ggtctcgtgg tagcgattgc
      541 tagtaatggg ggtggcaaac aggctcttga gactgttcag cgccttctac cagttctctg
      601 tcaagcccac ggcctgacgc ccgagcaagt tgtagcgatt gctagtaaca atggtggcaa
      661 acaggctctt gaaaccgtac agcgcctact gccagttctc tgtcaagccc acggtctgac
      721 tccggagcaa gttgtagtga gaccatatct ggtcgagcca cccatgacca aaatccctta
      781 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      841 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      901 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      961 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1021 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1081 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1141 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1201 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1261 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1321 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1381 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1441 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1501 cgttatccac agaatcaggg gataacgcag gaaagaacat gaattaattc tcatgtttga
     1561 cagcttatca tcgattagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt
     1621 caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg
     1681 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
     1741 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
     1801 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
     1861 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
     1921 tggattctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct
     1981 ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
     2041 agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc
     2101 atctccttac atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg
     2161 ggctgcttcc taatgcagga gtcgcataag ggagagcgcc gacccatgcc cttgagagcc
     2221 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     2281 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     2341 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     2401 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     2461 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     2521 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     2581 atgcccgcgt tgcaggccat gc
//
