LOCUS       sequence-361315-       11984 bp    DNA     circular SYN 14-APR-2025
DEFINITION  tdTomato expression from dox-inducible promoter; plasmid encodes the
            TetR inducer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, 
            Landthaler M, Gerst JE
  TITLE     Global analysis of contact-dependent human-to-mouse intercellular 
            mRNA and lncRNA transfer in cell culture.
  JOURNAL   Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584.
  PUBMED    37249209
REFERENCE   2  (bases 1 to 11984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-05-30"; volume: "12:e83584. doi"; pages: " 
            10.7554/eLife.83584"
COMMENT     Sequence Label: Inducible tdTomato
FEATURES             Location/Qualifiers
     source          1..11984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        372..570
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             588..768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    815..940
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1433..1666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1851..1895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2193..2310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(2354..2387)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2459..3670
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3507..3526
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3729..4346,4347..4352,4353..4388,4389..4391,4392..4427,
                     4428..4430,4431..4466,4467..4475)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        4530..4879
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    4538..4556
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4574..4592
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4610..4628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4646..4664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4682..4700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4718..4736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4754..4772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4818..4842
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4930..4939
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4936..6366
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(6373..6406)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6431..7019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6484..6504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6902..6913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7022..7038)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7023..7039)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7544..7724
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7750..7767)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7756..7980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8026..8454
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8113..8132)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8323..8344
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8463..8483)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8468..8796
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8647..8782
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8709..8728
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8844..8891
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8910..9284
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9414..9535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9451..9470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9505..9524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9584..9600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9584..9600)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9597..9619)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9608..9624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9632..9638,9639..9656,9657..9662))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9677..9698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9815..9832)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9986..10573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10065..10084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10744..11535,11536..11604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11367..11386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11605..11709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11784..11803)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(11975..11984,1..370)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      601 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      661 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      721 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      781 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      841 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      901 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      961 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1021 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1081 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1141 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1201 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1261 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1321 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1381 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1441 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1501 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1561 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1621 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1681 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1741 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1801 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1861 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1921 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1981 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2041 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2101 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2161 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2221 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2281 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2341 gtttggttaa ttaataactt cgtatagcat acattatacg aagttattaa aaaaaaagca
     2401 ccgactcggt gccacttttt caagttgata acggactgga gtaacccacc caagatctgg
     2461 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
     2521 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
     2581 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
     2641 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
     2701 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
     2761 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
     2821 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
     2881 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
     2941 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
     3001 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
     3061 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
     3121 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
     3181 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
     3241 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     3301 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     3361 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     3421 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     3481 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     3541 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     3601 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     3661 tttgttagac gaagcttggt accgagctcg gatctccacc ccgtaccggt cctgcagtcg
     3721 aattcaccat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     3781 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     3841 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     3901 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     3961 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     4021 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     4081 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     4141 gccactttac actaggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     4201 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     4261 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     4321 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     4381 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     4441 ttgacgattt tgaccttgac atgctccccg ggtaattaat taattaaccc gtgtcggctc
     4501 cagatcttga ggccctttcg tcttcactcg agtttactcc ctatcagtga tagagaacgt
     4561 atgaagagtt tactccctat cagtgataga gaacgtatgc agactttact ccctatcagt
     4621 gatagagaac gtataaggag tttactccct atcagtgata gagaacgtat gaccagttta
     4681 ctccctatca gtgatagaga acgtatctac agtttactcc ctatcagtga tagagaacgt
     4741 atatccagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
     4801 ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca
     4861 acacttttgt cttatactta cgcgtctgca ggatatcaag cgaagcttgg gctgcaggtc
     4921 gactctagag ccaccatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
     4981 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     5041 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     5101 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     5161 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     5221 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     5281 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     5341 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     5401 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     5461 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     5521 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5581 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcaccggcag caccggcagc
     5641 ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg
     5701 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     5761 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     5821 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     5881 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     5941 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     6001 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     6061 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     6121 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     6181 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     6241 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     6301 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac
     6361 aagtagtaat agataacttc gtatagcata cattatacga agttatgaat tcgatatcaa
     6421 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6481 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6541 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     6601 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     6661 aacccccact ggttggggca ttgccaccac ctgtcagctt ctttccggga ctttcgcttt
     6721 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6781 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6841 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6901 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6961 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     7021 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7081 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     7141 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7201 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     7261 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     7321 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     7381 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     7441 cctgtatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     7501 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     7561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     7741 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     7801 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7861 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7921 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7981 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8041 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8101 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8161 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8221 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8281 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8341 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8401 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtcccagg ctccccagca
     8581 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     8641 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     8701 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     8761 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     8821 attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag
     8881 tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc
     8941 tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc
     9001 gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca
     9061 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
     9121 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
     9181 gaccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
     9241 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
     9301 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     9361 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     9421 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9481 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9541 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     9601 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     9661 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     9721 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9781 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     9841 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     9901 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     9961 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg ccccctgacg agcatcacaa
    10021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    11041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11701 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
    11761 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
    11821 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
    11881 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
    11941 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gatt
//
