LOCUS       sequence-361320-        8571 bp    DNA     circular SYN 15-APR-2025
DEFINITION  For exrpression of GFP targeted to peroxisomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Haimovich G, Dasgupta S, Ravi AG, Gerst JE
  TITLE     Complementation of a human disease phenotype in vitro by 
            intercellular mRNA transfer
  JOURNAL   bioRxiv 2024.11.06.622258
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.11.06.622258"
COMMENT     Sequence Label: pHAGE-EGFP-SKL
FEATURES             Location/Qualifiers
     source          1..8571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(955..1746,1747..1815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1578..1597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1816..1920)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1946..2401)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2083..2104)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2295..2314
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2528..2550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2542..2559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2543..2559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2569..2588
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2569..2587
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2608..2743
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2783..2904
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2794..2813)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2848..2867
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             2976..3209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3296..3884
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3402..3413
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3811..3831
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(3912..4622,4623..4625,4626..4628))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3912..4622,4623..4625,4626..4628))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3954..3975)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4303..4322
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4562..4583
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4625..4634
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4668..5879)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4812..4831)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    5936..6053
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6320..6364)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6549..6782
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7275..7400
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7447..7627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7642..7845)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7671..7691)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7846..8225
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(8326..8346)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8326..8344)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8365..8381)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8365..8381)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8378..8400)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8389..8405
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8413..8419,8420..8437,8438..8443))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8458..8479
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
      901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
      961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     1921 cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg
     1981 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     2041 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     2101 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     2161 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     2221 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     2281 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     2341 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     2401 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     2461 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     2521 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
     2581 ctatagggcg aattgggtac gtccctaggc ctccaaaaaa gcctcctcac tacttctgga
     2641 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
     2701 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
     2761 ggatagctag agccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     2821 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     2881 attataagct gcaataaaca agttcctctc actctctgat attcatttct ttgcaagtta
     2941 taaatactga ataataagat gacatgaact actactgcta gagattttcc acactgacta
     3001 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     3061 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     3121 ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa gcagatcttg
     3181 tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa agtggctaag
     3241 atctacagct gccttgtaag tcattggtct taaaggtacc gagctcgaat tccaggcggg
     3301 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
     3361 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
     3421 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag
     3481 gacgtcagct tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
     3541 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
     3601 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
     3661 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
     3721 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
     3781 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     3841 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgac gcggccgctt
     3901 tagagcttgc tcttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     3961 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
     4021 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     4081 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     4141 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
     4201 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     4261 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     4321 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     4381 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     4441 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     4501 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     4561 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     4621 ctcaccatgg tggcgaccgg tggatctcga cctgcagccc aagcttcgtc taacaaaaaa
     4681 gccaaaaacg gccagaattt agcggacaat ttactagtct aacactgaaa attacatatt
     4741 gacccaaatg attacatttc aaaaggtgcc taaaaaactt cacaaaacac actcgccaac
     4801 cccgagcgca tagttcaaaa ccggagcttc agctacttaa gaagataggt acataaaacc
     4861 gaccaaagaa actgacgcct cacttatccc tcccctcacc agaggtccgg cgcctgtcga
     4921 ttcaggagag cctaccctag gcccgaaccc tgcgtcctgc gacggagaaa agcctaccgc
     4981 acacctaccg gcaggtggcc ccaccctgca ttataagcca acagaacggg tgacgtcacg
     5041 acacgacgag ggcgcgcgct cccaaaggta cgggtgcact gcccaacggc accgccataa
     5101 ctgccgcccc cgcaacagac gacaaaccga gttctccagt cagtgacaaa cttcacgtca
     5161 gggtccccag atggtgcccc agcccatctc acccgaataa gagctttccc gcattagcga
     5221 aggcctcaag accttgggtt cttgccgccc accatgcccc ccaccttgtt tcaacgacct
     5281 cacagcccgc ctcacaagcg tcttccattc aagactcggg aacagccgcc attttgctgc
     5341 gctcccccca acccccagtt cagggcaacc ttgctcgcgg acccagacta cagcccttgg
     5401 cggtctctcc acacgcttcc gtcccaccga gcggcccggc ggccacgaaa gccccggcca
     5461 gcccagcagc ccgctactca ccaagtgacg atcacagcga tccacaaaca agaaccgcga
     5521 cccaaatccc ggctgcgacg gaactagctg tgccacaccc ggcgcgtcct tatataatca
     5581 tcggcgttca ccgccccacg gagatccctc cgcagaatcg ccgagaaggg actacttttc
     5641 ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta gagtcaccca agtcccgtcc
     5701 taaaatgtcc ttctgctgat actggggttc taaggccgag tcttatgagc agcgggccgc
     5761 tgtcctgagc gtccgggcgg aaggatcagg acgctcgctg cgcccttcgt ctgacgtggc
     5821 agcgctcgcc gtgaggaggg gggcgcccgc gggaggcgcc aaaacccggc gcggaggcca
     5881 gatctggagc cgacacgggt taatcaagct tctcgaggct agtctcgtga tcgataaaat
     5941 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     6001 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc
     6061 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     6121 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     6181 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     6241 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     6301 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     6361 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     6421 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     6481 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     6541 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     6601 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     6661 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     6721 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     6781 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     6841 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     6901 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     6961 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     7021 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     7081 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     7141 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     7201 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     7261 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     7321 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     7381 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     7441 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     7501 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     7561 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     7621 gagaccccgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     7681 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     7741 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     7801 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     7861 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     7921 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     7981 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     8041 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     8101 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     8161 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     8221 atgtcaacat ggcggtaatg ttggacatga gccaatataa atgtacatat tatgatatgg
     8281 atacaacgta tgcaatggcc aagcttgcag ctccagcttt tgttcccttt agtgagggtt
     8341 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     8401 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     8461 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     8521 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt t
//
