LOCUS       sequence-361327-        9699 bp    DNA     circular SYN 15-APR-2025
DEFINITION  tdTomato with fragment nucleotides 1-440 of beta-actin 3'UTR 
            inserted in 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Dasgupta S, Dayagi DY, Haimovich G, Wyler E, Olender T, Singer RH, 
            Landthaler M, Gerst JE
  TITLE     Global analysis of contact-dependent human-to-mouse intercellular 
            mRNA and lncRNA transfer in cell culture.
  JOURNAL   Elife. 2023 May 30;12:e83584. doi: 10.7554/eLife.83584.
  PUBMED    37249209
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-05-30"; volume: "12:e83584. doi"; pages: " 
            10.7554/eLife.83584"
COMMENT     Sequence Label: tdTomato-beta-actin3'UTR1
FEATURES             Location/Qualifiers
     source          1..9699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2292..2671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2672..2875
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2826..2846
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2890..3070
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3117..3242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3735..3968
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4153..4197
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4464..4581
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4638..5849
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5686..5705
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5883..5892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5889..7319
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    7782..8370
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7835..7855)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8253..8264)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8457..8690
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8762..8883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8799..8818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8853..8872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8902..9037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9057..9076)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9058..9076)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9086..9103)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9086..9102)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9095..9117)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9244..9672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9331..9350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9541..9562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttggcc
     2221 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     2281 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2341 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2401 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2461 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2521 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2581 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2641 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2701 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2761 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2821 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     2881 agtgaaccgg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2941 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     3001 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     3061 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     3121 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     3181 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     3241 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3301 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3361 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3421 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3481 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3541 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     3601 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3661 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3721 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3781 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3841 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3901 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3961 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     4021 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     4081 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     4141 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     4201 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     4261 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4321 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     4381 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     4441 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
     4501 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     4561 attacaaaaa ttcaaaattt tatcgatcac gagactagcc tcgagaagct tgattaaccc
     4621 gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
     4681 cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc
     4741 ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca
     4801 gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt tctttccaga
     4861 gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg
     4921 gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc
     4981 gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag
     5041 tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa
     5101 gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact
     5161 gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct
     5221 tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc
     5281 caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg
     5341 caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct
     5401 gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg
     5461 cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc
     5521 acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag
     5581 ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc
     5641 gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt
     5701 gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa
     5761 tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat
     5821 tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga gatccaccgg
     5881 tcgccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     5941 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6001 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     6061 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     6121 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6181 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     6241 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     6301 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     6361 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     6421 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     6481 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     6541 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     6601 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     6661 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     6721 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     6781 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     6841 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     6901 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     6961 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     7021 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     7081 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     7141 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     7201 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7261 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagtagg
     7321 cggactgtta ctgagctgcg ttttacaccc tttctttgac aaaacctaac ttgcgcagaa
     7381 aaaaaaaaaa ataagagaca acattggcat ggctttgttt tttttaattt tttttaaagt
     7441 tttttttttt tttttttttt tttttttttt tttttttttt ttttttkgyg yttttkayty
     7501 aggwtttaaa aaytggaacg gtgaaggsga cagcagttgg ttggagcaaa catcccccaa
     7561 agttctacaa atgtggctga ggactttgta cattgttttk tttttttttt tttttntttt
     7621 tttggttttg tcttttttta atagtcattc caagtatcca tgaaataagt ggttacagga
     7681 agtccctcac cctcccaaaa gccaccccca ctcctaagag gaggatggtc gcgtccatgc
     7741 cctgagtcca ccccggggaa ggtgacagcg gccgcgtcga caatcaacct ctggattaca
     7801 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     7861 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     7921 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     7981 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     8041 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     8101 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     8161 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     8221 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     8281 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     8341 gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc
     8401 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8461 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8521 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8581 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8641 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8701 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8761 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8881 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagtyccgc ccattctccg
     8941 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9001 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     9061 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9121 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9181 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9241 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9661 ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
