LOCUS       sequence-361684-        5932 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Positive control for mosTI(unc-119). unc-119 rescue and pan-neuronal
            nuclear GFP expression after mosTI insertion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM416 - mosTI unc-119 P_UPN - gfp(nls) - tbb-2
FEATURES             Location/Qualifiers
     source          1..5932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     4..21
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5..21
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(645..678)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2198..2218
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      3159..3482
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(3841..3857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3841..3857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3854..3876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(4029..4617)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4109..4128)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(4734..4758)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4858..5649,5650..5718))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASQQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5481..5500
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5719..5823)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(5922..5932,1..12)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
       61 ttccgcctga cctaagtgct tttcgagatc gccaaaccgg agaacgatca ggagaatgat
      121 gagtcgccac aggagtcggc aagatacgtg cgttatagat ttgctccaaa ctttttgaaa
      181 ctcaaaacgg tcggagcaac tgtggaattc aaagtaggag acatcccaat ccatcatttc
      241 cgaatgatcg aacgtcactt cttcaaagat cgccttctga agtgttttga ctttgaattc
      301 ggattctgta ttccgaattc acgaaacaac tgtgaacata tctatgagtt ccctcaactc
      361 tctcaacaac tcagtgagtc attattctaa aaagtacaat tcaaaagact aatctctttt
      421 tcagtggacg acatgatcaa caatccaaac gagactcgtt ctgacagctt ctatttcgtc
      481 gataacaaac tcgtcatgca caacaaagcc gactactcat atgatgcata aatcttcatt
      541 gttgagttta tcttgttgat ttttgaataa attatcaact ctttactttt taatgggtta
      601 tgaaataaat aaacattgaa aactgataaa caacgttcat ctctataact tcgtatagca
      661 tacattatac gaagttatct gtgtctctcc gtcctatcca ctatctttca tccagattcc
      721 cggttttccc gagaaaaagt gagagagaga agaggctact gcgcggcgac actggctgcg
      781 gacaccctca ctcctctact tgtctccatc gattttcgct ggttcgtcga agcctcgtcg
      841 ccaacacaac ttcttcttct ttttcgtcct cttcttttct gccacacgct tgcacagctt
      901 cttcttctca ttttacgctg tctacttttt tcggaggggc cataccccca cacacagctc
      961 cttttatttg gcttctcttc ttcttcttgt ccatatactt cttaaaagtc tcgaacttct
     1021 ctctctcact ctccatcaat ttctttatgg ctaccgcttg cgctctgccg cttccgaaga
     1081 aagaaggcgt tggcagagct ctcaattcta ttttttctcg ccgtaggcta tcgatttctc
     1141 agcttctctt ctctcttcct cactccacca ccaccacttc gggcttcttc ttttcttcat
     1201 tttcgtcttc ttcttcatcc tcttcttttt ttttcagttc cccctcactc ctccccttat
     1261 atgcgcgtgc gagaggggtg caaaagcagc gcgcatccga gaattgaaac gagaaaacgg
     1321 agacgcagca gcagttcgtc ctcagaaaaa tagccagtaa aaagagaaaa agatagagat
     1381 agacctatcg cattttattt tcaaattgca attcctgcaa ttcatgtgtg cgtgtgccca
     1441 ttttcaattc ttcccgttat ttttcagacc attaccaacg ttctttctag atcttgagac
     1501 aatcttcctt ctgctcaatc gttcgtcgtg aagacggcat cgacgacgac gacgtagagg
     1561 gccgggactc ttcccactga aactcaatac ttttgcatat ttcacattct cttcctccgt
     1621 tggttcacca aaagagaaaa gggaccgaca tgaaaaggaa acgaaagctt aaaagagacg
     1681 gaaaatcgat gtactagagt gctcggaagt agttgagagg cgcagagaga gagctttcat
     1741 tcagaagaca cacgacgtta ttcctcgcaa cgccttcttc tttttaacaa tcttcaatac
     1801 attttagtga actttctttt atttctgttt catttaaaaa tagtcttaaa tttcactata
     1861 tttaattttt ttcttttatt atctggaatg tttcagcttt caacatgcca ttaaagagtg
     1921 tgctccacga gccggagaga cgcagatggg catgagaaga agtcgataag aaaggagggt
     1981 gccgtgctcc ctcaacgtcg tctgctccat cgccgaattt gtgctctttc gtcgagagag
     2041 ctcttcactt tgaactggcc aggatccccg ggattggcca aaggacccaa aggtatgttt
     2101 cgaatgatac taacataaca tagaacattt tcaggaggac ccttggaggt gtaccggttt
     2161 tcccagtcac gacgcgtggt acgagctcag aaaaatgcca aagaagaaga gaaaggtgtc
     2221 aaaaggagaa gaactcttta ccggagtcgt cccaattctc gtcgagctcg acggagacgt
     2281 aaacggacac aaattctcgg tttccggaga aggagaagga ggtaagtttt acgaaattct
     2341 gaagctcaca aaacatggag tacatttttc agatgctact tatggaaaac tcacccttaa
     2401 attcatttgc accaccggaa agctcccagt gccgtggccg acccttgtga ctacattctg
     2461 ctacggagtt caatgctttt cccgctaccc agaccacatg aagcgtcacg atttcttcaa
     2521 atcagccatg ccagaaggat acgtccagga gcgaacaatt ttcttcaagg acgatggaaa
     2581 ctacaagact cgtgctgaag tcaagtttga aggagatact ctcgtgaacc gcattgagct
     2641 caaggtaagt ttataatcta tgatcttaat ttggcaatgg aaaaagtttt ttcagggaat
     2701 cgacttcaaa gaagatggaa acattcttgg acacaagctc gaatacaact ataactcgca
     2761 caacgtgtat atcatggccg acaagcaaaa aaatggaatc aaggtcaact tcaagattcg
     2821 ccacaacatc gaagacgggt cggttcaact cgctgatcac taccagcaga acacaccaat
     2881 tggaggtaag ttttattcca taatacaaat atatattgaa caataatgct tttcagatgg
     2941 accagtcctc ctccctgata atcactacct ttccactcaa tccgctctta gcaaggatcc
     3001 aaatgagaaa agagatcaca tggttcttct cgagtttgtc accgccgccg gaatcaccca
     3061 cggaatggac gagctttaca agtcgagacg ccgcaaggct aatccaacca aactttctga
     3121 aaatgccaag aaacttgcca aggaggtcga gaactgagat gcaaaatcct ttcaagcatt
     3181 cccttcttct ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg
     3241 cttttttaat gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg
     3301 aagtgaatgc tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc
     3361 cattttgaag tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta
     3421 ttgtgttcgt tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag
     3481 tctcacagga aacggagaat ctgtgcggca atgtcttatc atttgaaacg cgccgtatcg
     3541 atgctgacat catgcggctc ccgtgttagt cgagaagatg ggtacgggcc tttattcatc
     3601 caacactcta cggcttcttc aagagctagt agggcaccct gtagttggaa agggattatt
     3661 tcgtatggcg agcccatacc gtctttcttg cggaagctta acacgatagg aagttggata
     3721 gtttcgaacg atggttccat acggaacgct gtctcaagga tgagtctgac ggagtgtaac
     3781 tcgaaggcta ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg
     3841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg
     3901 ctatccgaca atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct
     3961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     4621 gatcctttga tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca
     4681 agtcagcgta aatgggtagg gggcttcaaa tcgtcctcgt gataccaatt cggagcctgc
     4741 ttttttgtac aaacttgttg ataatggcaa ttcaaggatc ttcacctaga tccttttaaa
     4801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     4861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     4921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     4981 tgctgcaatg ataccgcgag agccacgctc accggctcca gatttatcag caataaacca
     5041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5101 tattaattgt tgctgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     5461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     5761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     5821 gcgcacattt ccccgaaaag tgccagatac ctgaaacaaa acccatcgta cggccaagga
     5881 agtctccaat aactgtgatc caccacaagc gccagggttt tcccagtcac ga
//
