LOCUS       sequence-361791-       17381 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion 
            protein under the maternal tubulin promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17381)
  AUTHORS   Singh AP, Wu P, Ryabichko S, Raimundo J, Swan M, Wieschaus E, Gregor
            T, Toettcher JE
  TITLE     Optogenetic control of the Bicoid morphogen reveals fast and slow 
            modes of gap gene regulation.
  JOURNAL   Cell Rep. 2022 Mar 22;38(12):110543. doi: 
            10.1016/j.celrep.2022.110543.
  PUBMED    35320726
REFERENCE   2  (bases 1 to 17381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-03-22"; volume: "38(12):110543. doi"; pages: " 
            10.1016/j.celrep.2022.110543"
COMMENT     Sequence Label: pBABR IRFP-Bcd-LEXY
FEATURES             Location/Qualifiers
     source          1..17381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2443..3390
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     protein_bind    5380..5427
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    8162..8209
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9053..9069
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9053..9069
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    9439..9508
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(9672..9873,9874..9904)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(10442..10461)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10561..10583
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10621..10639)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10707..10811
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10812..10880,10881..11672)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11030..11049)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11843..12431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12332..12351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(12666..12696,12697..13251)
                     /label=P element 5' end
     CDS             complement(join(14030..14644,14715..14846,15050..15365,
                     15427..16081,16156..16429,16831..16902))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     17354..17372
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 aattggccgc tctagccccc cctcgaatgt tctctctctt ctcttctctc tctctttctc
       61 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctaag
      121 ggtcgagtcg atagccgaag cttaccgaag tatacactta aattcagtgc acgtttgctt
      181 gttgagagga aaggttgtgt gcggacgaat ttttttttga aaaccggtcc acggccgctt
      241 ggagggagat tagcaaaacg cagcgcgtga tcaaggtgac aatgaagcca ccgaccacca
      301 cggtggcgcc caacaaggca cgagtggttt gcatgtccga cacgcactcc ctgacgccct
      361 acatcaagtt cgatatacca gatggcgata tattcataca tgccggcgat tttaccaagt
      421 gcggccaact ggaggaggtg gaggagttta acacctggat tggagcactg ccccatcgcc
      481 acaagatcgt gattgctggc aatcatgagc tcagcttcga tagaaccttc acccatcctt
      541 tccagaagag caaaagtcat gcttcgagca gcaaacacac cggcatgtcc atcctcgatg
      601 atctgcccac tttgggcaac gccaaggaga acttggagag cgctgtgcaa acgcagaatg
      661 tccgcgatgt tctgaccaac tgtcggtatt tggaggatga actacttgag atttggggta
      721 ttcaaatata tggctcgcca tggcaaccgg aattctgtcg ctgggccttt aatgttccac
      781 gtggaactgc ctgtttggat aagtggaatc aaatcccgga aggcatcgat atcctggtca
      841 cccacacgcc gcccgtgggt cacggcgatc tctgctgttc cggcgtacga gccggttgtg
      901 tggagctact cagcacagtg cagcaacgag tgcgcccgaa ataccatgtc ttcggacatg
      961 tacacgaagg ctacggcatc accagcgatg gcaggatcat ctttgtgaat gcctcaacgt
     1021 gtgacatcaa ctacctaccc aacaatgccc ccatcgtttt cgatgtgacg ctgccccccg
     1081 gatttcgcaa ggattagcat gcctagatga ttatttattg tgtcacaggt tcaatgaatt
     1141 tattttcgtc ccactttatg tttatgttaa ggtatttcgt ctgtaaagat tttgaagcac
     1201 aattgtaggt gtctagagag atagaattca attcgcacga aattaaaaaa agaaattcac
     1261 acttaagatg ccataaaata tttattcaag tagagtgcat ttgtcaatat atgaatttgt
     1321 aaaaataaac aagttttgaa gacacctaaa cttttgaaga ttttcaatat gatattatat
     1381 agtttttaat aataatttaa gtaaaggtaa agcaaatcct gagatacata catactattt
     1441 caaattagta tcgataagcc gccagaaatg ggttggaaaa accttggaat gattattggt
     1501 tagcagccct gtctcccaat ttgacattcc tctttggcgc tctgtaacgg cgcttttcca
     1561 gtgactcccc aatcagcgaa ccaactaacc tgaggctgca cttttcgcgt ggcgaccaaa
     1621 aaaattataa aaaaaaggtc ccgcattaga ttttaatttg tatacaccca attttagtcc
     1681 agcaaaaaaa aaaactaatc aattggtgag aagaagaaaa actaattagt ttaagaattg
     1741 ctgaaagcgg gaagtgaaat ttaaaatcag aaaagatgtg taagaagatg tgaaatgaaa
     1801 aggtgtggga agtgaacatg aataaatgtt cattgaaaat gaacagctaa ttgcgaattt
     1861 tttgaaactt aactgttaac tgttactgct cacaaatcct tgaaaaatat taagatcgat
     1921 cttagaattg tttatttaaa atgaattcct cgtatgtatt ataacagaca atcaagttta
     1981 ttaaagaata tttaaacttg tatttttttt tgagtttgat aaaatgtccc aaatataaaa
     2041 gagtattacc ttttcgtcat ataatactgt tcttaatatt agttttagtt agtaatgtgt
     2101 ttataaagta aattacaaag ggaggaccta acctaacctt aaacaatggc gccttctctt
     2161 gcccagctca tcacttttat tgtttcaccc gccagcgcga agtagtgtcc atccagattg
     2221 ggacggtacc atctcttcgc tcatccctaa ataacggcac tctgcagatg cgaagcagtg
     2281 gatcgcaaaa acgcaaaatg tgggcgaaat aagttcgcga gcgtctcgaa agtatccggt
     2341 tactgaaaat acaagaaagt ttccacactc ctttgccatt tttccgcgcg gcgcttggaa
     2401 attcgtaaag ataacgcggc ggagtgtttg gggctagcaa agatggctga aggatccgtc
     2461 gccaggcagc ctgacctctt gacctgcgac gatgagccga tccatatccc cggtgccatc
     2521 caaccgcatg gactgctgct cgccctcgcc gccgacatga cgatcgttgc cggcagcgac
     2581 aaccttcccg aactcaccgg actggcgatc ggcgccctga tcggccgctc tgcggccgat
     2641 gtcttcgact cggagacgca caaccgtctg acgatcgcct tggccgagcc cggggcggcc
     2701 gtcggagcac cgatcactgt cggcttcacg atgcgaaagg acgcaggctt catcggctcc
     2761 tggcatcgcc atgatcagct catcttcctc gagctcgagc ctccccagcg ggacgtcgcc
     2821 gagccgcagg cgttcttccg ccgcaccaac agcgccatcc gccgcctgca ggccgccgaa
     2881 accttggaaa gcgcctgcgc cgccgcggcg caagaggtgc ggaagattac cggcttcgat
     2941 cgggtgatga tctatcgctt cgcctccgac ttcagcggcg aagtgatcgc agaggatcgg
     3001 tgcgccgagg tcgagtcaaa actaggcctg cactatcctg cctcaaccgt gccggcgcag
     3061 gcccgtcggc tctataccat caacccggta cggatcattc ccgatatcaa ttatcggccg
     3121 gtgccggtca ccccagacct caatccggtc accgggcggc cgattgatct tagcttcgcc
     3181 atcctgcgca gcgtctcgcc cgtccatctg gaattcatgc gcaacatagg catgcacggc
     3241 acgatgtcga tctcgatttt gcgcggcgag cgactgtggg gattgatcgt ttgccatcac
     3301 cgaacgccgt actacgtcga tctcgatggc cgccaagcct gcgagctagt cgcccaggtt
     3361 ctggcctggc agatcggcgt gatggaagag gagttcgact ccgcaggctc cgccggaagt
     3421 gcgggaggta gtagtgcgca accgccgcca gatcaaaact tttaccatca tccgctgccc
     3481 cacacgcaca cacatccgca tccgcactcc catccgcatc cgcactcgca tccgcaccca
     3541 catcaccaac atccgcagct tcagttgccg ccacaattcc gaaatccctt cgatttgctt
     3601 ttcgatgagc gaacgggagc gataaactac aactacatac gtccgtatct gcccaaccag
     3661 atgcccaagc cagaggagct gcccgactct ctggtgatgc ggcgaccacg tcgcacccgc
     3721 accactttta ccagctctca aatagcagag ctggagcagc actttctgca gggacgatac
     3781 ctcacagccc cccgacttgc ggatctgtca gcgaaactag ccctgggcac agcccaggtg
     3841 aagatatggt ttaagaaccg tcggcgtcgt cacaagatcc aatcggatca gcacaaggac
     3901 cagtcctacg aggggatgcc tctctcgccg ggtatgaaac agagcgatgg cgatcccccc
     3961 agcttgcaga ctcttagctt gggtggaggg gccacgccca acgctttgac tccgtcaccc
     4021 acgccctcaa cgcccactgc acacatgacg gagcactaca gcgagtcatt caacgcctac
     4081 tacaactaca atggaggcca caatcacgcc caggccaatc gtcacatgca catgcagtat
     4141 ccttccggag ggggggcagg acctgggtcg accaatgtca atggcggcca gttcttccag
     4201 cagcagcagg tccataatca ccagcagcaa ctgcaccacc agggcaacca cgtgccgcac
     4261 cagatgcagc agcagcaaca gcaggctcag cagcagcaat accatcactt tgacttccag
     4321 caaaagcaag ccagcgcctg tcgcgtcctg gtcaaggacg aaccggaggc cgactacaac
     4381 ttcaacagct cgtactacat gcgatcggga atgtctggcg ccactgcatc ggcatccgct
     4441 gtggcccgag gcgctgcctc gccgggctcc gaggtctacg agccattaac acccaagaat
     4501 gacgaaagtc cgagtctgtg tggcatcggc atcggcggac cttgcgccat cgccgttggc
     4561 gagacggagg cggccgacga catggacgac ggaacgagca agaagacgac gctacagatc
     4621 ttggagcctt tgaagggtct ggacaagagc tgcgacgatg gcagtagcga cgacatgagc
     4681 accggaataa gagccttagc aggaaccgga aatcgtggag cggcatttgc caaatttggc
     4741 aagccttcgc ccccacaagg ccctcagccg cccctcggga tggggggcgt ggccatgggc
     4801 gaatcgaacc aatatcaatg cacgatggat acgataatgc aagcgtataa tccccatcgg
     4861 aacgccgcgg gcaactcgca gtttgcctac tgcttcaata agggtggatc tggaggttca
     4921 ggtggaagtt tggctactac acttgaacgt attgagaaga actttgtcat tactgaccca
     4981 agattgccag ataatcccat tatattcgcg tccgatagtt tcttgcagtt gacagaatat
     5041 agccgtgaag aaattttggg aagaaactgc aggtttctac aaggtcctga aactgatcgc
     5101 gcgacagtga gaaaaattag agatgccata gataaccaaa cagaggtcac tgttcagctg
     5161 attaattata caaagagtgg taaaaagttc tggaacctct ttcacttgca gcctatgcga
     5221 gatcagaagg gagatgtcca gtactttatt ggggttcagt tggatggaac tgagcatgtc
     5281 cgagatgctg ccgagagaga gggagtcatg ctgattaaga aaactgcaga aaatattgac
     5341 gagctgctga aggagctggc cgacctgaac ctggactagg aagtacctat tccgaagttc
     5401 ctattctcta gaaagtatag gaacttccct ggatgagagg cgtgttagag agtttcatta
     5461 gctttaggtt aaccactgtt gttcctgatt gtacaaatac caagtgattg tagatatcta
     5521 cgcgtagaaa gttaggtcta gtcctaagat ccgtgtaaat ggttcccagg gaagttttat
     5581 gtactagcct agtcagcagg ccgcacggat tccagtgcat atcttagtga tactccagtt
     5641 aactctatac tttccctgca atacgctatt cgccttagat gtatctgggt ggctgctcca
     5701 ctgaagcccg ggaatatgca accagttaca tttgaggcca tttgggctta agcgtattcc
     5761 atggaaagtt atcgtcccac atttcggaaa ttatattccg agccagcaag aaaatcttct
     5821 ctgttacaat ttgacatagc taaaaactgt actaatcaaa atgaaaaatg tttctcttgg
     5881 gcgtaatctc atacaatgat tacccttaaa gatcgaacat ttaaacaata atatttgata
     5941 tgatattttc aatttctatg ctatgccaaa gtgtctgaca taatcaaaca tttgcgcatt
     6001 ctttgaccaa gaatagtcag caaattgtat tttcaatcaa tgcagaccat ttgtttcaga
     6061 ttcggagatt ttttgctgcc aaacggaata actatcatag ctcacattct aattacatca
     6121 ctaagaagag cattgcaatc tgttaggcct caagtttaat tttaaaatgc tgcacctttg
     6181 atgttgtctc tttaagcttt gtatttttaa ttacgaaaat atataagaac tactctactc
     6241 gggtaaattg tgactaacta cacataacta catacttagc ccatatttcc gtccctttct
     6301 agaatgaacg aaaacagtat ctggttttcc cgaaaatctt atgaatttaa aaatgcactt
     6361 tattgcacat actcacacat gcctgccata aaatatgatt cgcgagcggc cgcggatcta
     6421 attcaattag agactaattc aattagagct aattcaatta ggatccaagc ttatcgattt
     6481 cgaaccctcg accgccggag tataaataga ggcgcttcgt ctacggagcg acaattcaat
     6541 tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct
     6601 gaacaagcta aacaatcggg cggccgcact agagccggtc gccaccatga ggtcttccaa
     6661 gaatgttatc aaggagttca tgaggtttaa ggttcgcatg gaaggaacgg tcaatgggca
     6721 cgagtttgaa atagaaggcg aaggagaggg gaggccatac gaaggccaca ataccgtaaa
     6781 gcttaaggta accaaggggg gacctttgcc atttgcttgg gatattttgt caccacaatt
     6841 tcagtatgga agcaaggtat atgtcaagca ccctgccgac ataccagact ataaaaagct
     6901 gtcatttcct gaaggattta aatgggaaag ggtcatgaac tttgaagacg gtggcgtcgt
     6961 tactgtaacc caggattcca gtttgcagga tggctgtttc atctacaagg tcaagttcat
     7021 tggcgtgaac tttccttccg atggacctgt tatgcaaaag aagacaatgg gctgggaagc
     7081 cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa ggagagattc ataaggctct
     7141 gaagctgaaa gacggtggtc attacctagt tgaattcaaa agtatttaca tggcaaagaa
     7201 gcctgtgcag ctaccagggt actactatgt tgactccaaa ctggatataa caagccacaa
     7261 cgaagactat acaatcgttg agcagtatga aagaaccgag ggacgccacc atctgttcct
     7321 ttagcggcca tcgaattcga gctcgcccac taagcgtcgc gccacttcaa cgctcgatgg
     7381 gagcgtcatt ggtgggcggg gtaaccgtcg aaatcagtgt ttacgcttcc aatcgcaaca
     7441 aaaaattcac tgcaacactg aaaagcatac gaaaacgatg aagattgtac gagaaaccat
     7501 aaagtatttt atccacaaag acacgtatag cagaaaagcc aagttaactc ggcgataagt
     7561 tgtgtacaca agaataaaat cggccagatt cagtgttgtc agaaataaga aaaccccact
     7621 atgtttttct ttgccttttc tttctcccag cgatcattca tttcgtggtg aaagaacggg
     7681 gtcattgcac ggagtttcga ctgcgggaaa gcagagctgc cgttcacttc gtctataatt
     7741 agcgctttct attttccccg attcgggccg ctgctgcgct tttccgcctg ctgtttgtgg
     7801 caagtgtagc agcaggctgt gcacgcagtg tggcatgcac ttggctttcc accgttggta
     7861 tcgattctct gggacgatga gtcattcctt tcggggccac agcataatcg ttgccagctc
     7921 accgaaatgg tgacttcatt tcttaactgc cgtcaagcat gcgattgtac atacatacat
     7981 atttatatat gtacatattt atgtgactat ggtaggtcga tataatagca atcaacgcaa
     8041 gcaaatgtgt cagtcctgct tacaggaacg attctattta gtaattttcg ttgtataaag
     8101 taattatgta tgtatgtaag ccccataaat ctgaaacaat taggcaaaac catgcgaagc
     8161 tgaagtacct attccgaagt tcctattctc tagaaagtat aggaacttcc atatggcggc
     8221 cgccattcgt agaataggga tccccagcag tcgattcact agccagctgc ctctccaatc
     8281 agcctgtcca aactatccat catgagtttc gttttgataa tttaatatat acaagtataa
     8341 attttttttt tttggtttcg cgccttctgt tctttgattc ctgataatcc tgtacttatc
     8401 cgtgcatttg aattaaacaa aaagtagcca acaccagcga ttgttatgta cgacagtgta
     8461 gtgtgtgaga tgttggtgcc agataacacg atttcccgaa aaactccttt cataagtatt
     8521 aactcattga atatacatat atattaccta tatacaaata catacttata caagaatgtg
     8581 gcaataaacc tttgtttttc ttacactcga gaacgtgcgc acgtccaacc aaacagatgt
     8641 ctgcagaaat ccttcagcca aaagcaactg atcaaacaat agaaactttg gaatatgtac
     8701 cccgcatggc taactaagca tgatcactag catgatgtga taaaagtatg tagcttcaaa
     8761 tgtggaggga aattgaaaga gatttcggaa attagtcaaa attttcttgg aaccttcgac
     8821 aagctgacta atgagttcat gtccaaaaga ctcgaacttg taaactcata tcttatctca
     8881 cattattttt gattcgaaga tatttcagct tctgcaaata tatgtttttc aagaatttat
     8941 ttaaaaaaaa aatcaattaa atagatggct taatgttcga aacactcaac gttgtatatt
     9001 acacactttt tggacaacta ttaatttaaa agaaaacacg aacatttgaa aatctagaac
     9061 tagtggatct ggatccagac tgcagagaag atctatagaa ttcagagagg ccggcagaga
     9121 ggctagcagg catgcatcta gagtcgacct cgaacgttaa cgttaacgta acgttaactc
     9181 gaggagcttg ataacattat acctaaaccc atggtcaaga gtaaacattt ctgcctttga
     9241 agttgagaac acaattaagc atcccctggt taaacctgct tgatagtgca ggctatacag
     9301 aactgcgcta gtgactccta tccgcctgcg tttctctgcc tgctcacggg tcatatgcgt
     9361 gctttggagc tgagggtgcg gcggattggc gtcgacgatg taggtcacgg cgggcatgtc
     9421 gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
     9481 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
     9541 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
     9601 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg acgatgtagg tcacggcggg
     9661 cagccaagct ttgcgtactc gcaaattatt aaaaataaaa ctttaaaaat aatttcgtct
     9721 aattaatatt atgagttaat tcaaacccca cggacatgct aagggttaat caacaatcat
     9781 atcgctgtct cactcagact caatacgaca ctcagaatac tattcctttc actcgcactt
     9841 attgcaagca tacgttaagt ggatgtctct tgccgacggg accaccttat gttatttcat
     9901 catggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc tctaagactt
     9961 cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt tttttttttt
    10021 accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat gtaattatat
    10081 ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct tcgaacatcc
    10141 ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg cataccttac
    10201 atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata tacatacttt
    10261 tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt agttgctctt
    10321 tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga ccttggagcg
    10381 actgtcgtta gttccgcgcg attcggtgcg gtatttcaca ccgcatatgg tgcactctca
    10441 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    10501 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    10561 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
    10621 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
    10681 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10741 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10801 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10861 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10921 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10981 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    11041 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    11101 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    11161 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    11221 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11281 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11341 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11401 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11461 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11521 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11581 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11641 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11701 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11761 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11821 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11881 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11941 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    12001 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    12061 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    12121 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    12181 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12241 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12301 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12361 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12421 gcctatggaa aaacgccttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
    12481 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
    12541 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
    12601 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
    12661 tggcccatga tgaaataaca taaggtggtc ccgtcgatag ccgaagctta ccgaagtata
    12721 cacttaaatt cagtgcacgt ttgcttgttg agaggaaagg ttgtgtgcgg acgaattttt
    12781 ttttgaaaac attaaccctt acgtggaata aaaaaaaatg aaatattgca aattttgctg
    12841 caaagctgtg actggagtaa aattaattca cgtgccgaag tgtgctatta agagaaaatt
    12901 gtgggagcag agccttgggt gcagccttgg tgaaaactcc caaatttgtg atacccactt
    12961 taatgattcg cagtggaagg ctgcacctgc aaaaggtcag acatttaaaa ggaggcgact
    13021 caacgcagat gccgtaccta gtaaagtgat agagcctgaa ccagaaaaga taaaagaagg
    13081 ctataccagt gggagtacac aaacagagta agtttgaata gtaaaaaaaa tcatttatgt
    13141 aaacaataac gtgactgtgc gttaggtcct gttcattgtt taatgaaaat aagagcttga
    13201 gggaaaaaat tcgtactttg gagtacgaaa tgcgtcgttt agagcagcag ccgaattaat
    13261 tctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg
    13321 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac
    13381 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac
    13441 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga
    13501 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa
    13561 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac
    13621 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
    13681 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
    13741 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
    13801 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
    13861 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
    13921 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
    13981 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
    14041 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
    14101 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
    14161 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
    14221 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
    14281 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca
    14341 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc
    14401 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc
    14461 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga
    14521 agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg
    14581 tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga
    14641 acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc
    14701 taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag
    14761 aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag
    14821 atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat
    14881 attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt
    14941 gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt
    15001 tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa
    15061 taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga
    15121 ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca
    15181 gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa
    15241 tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca
    15301 caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac
    15361 ccacgctgga taggagttga gatgttatgt aatactagat acccttaata aacacatcga
    15421 actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc
    15481 tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg
    15541 gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg
    15601 gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt
    15661 agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg
    15721 ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga
    15781 tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg
    15841 aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag
    15901 gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg
    15961 gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc
    16021 tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa
    16081 actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg
    16141 cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt
    16201 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
    16261 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
    16321 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
    16381 gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata
    16441 ggggatctca aatatcaact acaagcgtta tgctcatcta accccgaaca aaaagtaccc
    16501 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
    16561 ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt
    16621 tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca
    16681 gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt
    16741 cgcagttctc acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga
    16801 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
    16861 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
    16921 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
    16981 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
    17041 ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg
    17101 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
    17161 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
    17221 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
    17281 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
    17341 tttccgcaaa aatgggtttt attaacttac atacatacta g
//
