LOCUS       sequence-362084-        3064 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  to see protein dynamics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Fernandez-Nicolas A, Xu D, Yajima M
  TITLE     A tumor suppressor Retinoblastoma1 is essential for embryonic 
            development in the sea urchin.
  JOURNAL   Dev Dyn. 2019 Dec;248(12):1273-1285. doi: 10.1002/dvdy.113. Epub 
            2019 Oct 1.
  PUBMED    31515896
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.113"; 
            journalName: "Dev Dyn"; date: "2019-12"; volume: "248"; issue: "12";
            pages: "1273-1285"
COMMENT     Sequence Label: SpVasa-S636K-GFP
FEATURES             Location/Qualifiers
     source          1..3064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2203..2205,2206..2208,2209..2922)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSXEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2248..2269)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2509..2528)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2856..2877
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtcagaag actgggggac ctcttcggtg tcgggtggat ccagctttgg aaagagtagt
       61 tttggtggat caagtggtgg ggtcaaagac gatggatttg gcgttggagt cggtcgaggt
      121 cgaggaaggg gtcgcgggtt tagccccttt gcttcaaacg gtggtgtcgg tggtatgacc
      181 aatgggacta ccaatggagg aggagagagt agcggtgggt gggatgagag cccatctaag
      241 agtggctcaa gtagcagtcc atggggcggt gatggtgata atgggttcgg caagagtagc
      301 tttggtagca aatcgttcgg tggaggagga ggaggtggtg gtggtggggg ccgaggtgct
      361 gaatcaggag gaggacgagg aggaggagga ttcggcggca gacgaggagg acgtggaggt
      421 ggagatagat cctgttataa ctgcggtgaa acaggtcaca tgtcaagaga atgtcccaca
      481 aaggactctt caggaggagg acgtggtggt ggtggtggcg atagaagttg ctacaattgc
      541 ggtgagaccg gacacatgtc acgagaatgt cccacaaaag actcttcggg tggaggaggt
      601 ggaggtggtg gaaagtgctt tagatgtcaa gaagaaggcc actttgccaa ggaatgcccc
      661 aatccagata caagaaatga aggcgaaggt ggtgaagcca agccgccagc atccacctac
      721 gttcctcccg acccatccga ggaagaatca cagatttaca taagcactga acaaggaatc
      781 aacttcaaca agtatgatga tatacccgta gaagtcagcg gtcgagacag gccaaaaaat
      841 atacgagctt ttgaggaggc cgagctagat gagacggtat tgtccaatgt gcgcaaggct
      901 cactacgcta agccaacacc tgtgcagaag tatggtatgc ccatcatttc ttgtggacgt
      961 gatctgatgg cctgtgccca aacaggatca ggaaagacgg cggcattcct cctacccatc
     1021 atcaccaaca tgatcaccca cggaggatgc atcagcacct tcaacgtgat ccaggaacca
     1081 ctggccctca tcgtctcgcc aacccgcgag ctggccatcc agatctacaa cgaggcacgc
     1141 aagttctgcc gcggcaccac catacgacct gtggtggtat acggaggcac cagcgtcagt
     1201 catcagatga gagagtgtca gaagggatgc tccatcctgg tagccacccc aggacgtatg
     1261 catgacttcg taaccagagg atatatcggc ctgggcaagc tgaagttcct gatccttgac
     1321 gaagccgacc gtatgttgga catgggtttc ggtcccgaca tccagagact gatcgagcac
     1381 cctcacatgc ctgccaaggg tgatcgtcaa accctgatgt tcagtgcgac cttccccaat
     1441 gaggtccagg agaaggcagg ggagtacctc aacgactacc tcttcctgac ggtagggagg
     1501 gtaggaggag ctgcctcaga catcgagcag agggtgttct cggtcggtca attcgacaag
     1561 cgggataagc tgatggagat cctgggggat cagaaagatg atgaccgtac tctggtgttt
     1621 gtttcgacca agaggaatgc tgatttcttg gcctctcttc tcagtcaatc tgaattcccc
     1681 accaccagca tccatggtga ccgtcagcag caggagcgtg aggaagccct gcgtgacttc
     1741 aagaccggca aggcaccaat ccttgttgcc acgtccgtcg ccgccagagg tctggacatc
     1801 ccaggggtca agcacgtggt caactacgac ctcccaagcg acatcgatga gtacgtgcat
     1861 cgcatcgggc gtaccggacg tgtcggaaac cttggacgat caactaagtt ctatgatgca
     1921 gaaaaggatg ctaacattgc gagagctctc attaagatct tggctgatgc gcagcaggaa
     1981 gtaccggagt tcttagagga ggctgctgat tcagccattg gaacctacca tggcaacgct
     2041 ggagggagct ttggaggcag ggacaccagg aagttcggtg gtcgaggagg aggaagggga
     2101 ggaggaagtg gcggcggtgg tggagacgag tggggatctg gaggaggagg cggcggcggt
     2161 ggcggaggag gagctgctga tgatgaatca tgggatacta gtatggtgag caagggcgag
     2221 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2281 aagttcagcg tgtccgncga gggcgagggc gatgccacnt acggcaagct gaccctgaag
     2341 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacn
     2401 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     2461 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2521 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2581 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2641 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2701 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2761 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2821 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2881 gccgccggga tcactctcgg catggacgag ctgtacaagt aactagtgac tgactaggat
     2941 ctggttacca ctaaaccagc ctcaagaaca cccgaatgga gtctctaagc tacataatac
     3001 cacttacact ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata
     3061 aaaa
//
