LOCUS       sequence-362581-        9681 bp    DNA     circular SYN 15-APR-2025
DEFINITION  DEAA UPF1 inserted with lambdaN tagged on the N-terminus to use with
            the Tethering Assay (BoxB).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Fischer JW, Busa VF, Shao Y, Leung AKL
  TITLE     Structure-Mediated RNA Decay by UPF1 and G3BP1.
  JOURNAL   Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi: 
            10.1016/j.molcel.2020.01.021. Epub 2020 Feb 3.
  PUBMED    32017897
REFERENCE   2  (bases 1 to 9681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.021"; journalName: "Mol Cell"; date: 
            "2020-04-2- 2"; volume: "78"; issue: "1"; pages: "70-84.e6"
COMMENT     Sequence Label: pCI-neo-lambdaN-UPF1-DEAA
FEATURES             Location/Qualifiers
     source          1..9681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..413
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        56..247
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(79..99)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      264..399
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             464..1258
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(518..537)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1128..1147
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1322..1370
                     /note="synthetic polyadenylation signal"
     promoter        complement(1446..1464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1478..1494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1478..1494)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1491..1513)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1502..1518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1526..1532,1533..1550,1551..1556))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1571..1592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1709..1726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1880..2468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1960..1979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2639..3430,3431..3499))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3262..3281
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3500..3604)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3631..4086
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4212..4234
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4226..4243
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4227..4243
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4404..4783
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4784..4987
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4938..4958
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4984..5008
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5148..5280
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5263..5282
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5325..5343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    8858..8979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8869..8888)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8923..8942
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      9165..9620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag
       61 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
      121 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
      181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
      241 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
      301 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
      361 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
      421 attcttctga cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg
      481 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
      541 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
      601 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
      661 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
      721 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
      781 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
      841 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
      901 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
      961 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     1021 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     1081 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     1141 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     1201 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     1261 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg
     1321 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc
     1381 gataaggatc ccctaggctt ttgcaaaaag ctcctcgagg aactgaaaaa ccagaaagtt
     1441 aattccctat agtgagtcgt attaaattcg taatcatgtc atagctgttt cctgtgtgaa
     1501 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     1561 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     1621 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     1681 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     1741 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     1801 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     1861 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     1921 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     1981 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2041 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2101 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2161 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2221 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2281 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2341 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2401 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2461 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     2521 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     2581 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     2641 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     2701 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     2761 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     2821 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     2881 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     2941 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3001 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3061 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3121 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3181 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3241 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     3301 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     3361 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     3421 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     3481 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     3541 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     3601 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     3661 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3721 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3781 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3841 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3901 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3961 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4021 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4081 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4141 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     4201 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgatctatac
     4261 attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat aaatcaatat
     4321 tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt atattggctc
     4381 atgtccaata tgaccgccat gttgacattg attattgact agttattaat agtaatcaat
     4441 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     4501 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     4561 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     4621 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc ctattgacgt
     4681 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac gggactttcc
     4741 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     4801 gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     4861 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     4921 taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     4981 cagagctcgt ttagtgaacc gtcagatcac tagaagcttt attgcggtag tttatcacag
     5041 ttaaattgct aacgcagtca gtgcttctga cacaacagtc tcgaacttaa gctgcagtga
     5101 ctctcttaag gtagccttgc agaagttggt cgtgaggcac tgggcaggta agtatcaagg
     5161 ttacaagaca ggtttaagga gaccaataga aactgggctt gtcgagacag agaagactct
     5221 tgcgtttctg ataggcacct attggtctta ctgacatcca ctttgccttt ctctccacag
     5281 gtgtccactc ccagttcaat tacagctctt aaggctagag tacttaatac gactcactat
     5341 aggctagcca ccatgaacgc acgaacacga cgacgtgagc gtcgcgctga gaaacaagct
     5401 caatggaaag ctgcaaacgc ttagcgtgga ggcgtacggg cccagctcgc agactctcac
     5461 tttcctggac acggaggagg ccgagctgct tggcgccgac acacagggct ccgagttcga
     5521 gttcaccgac tttactcttc ctagccagac gcagacgccc cccggcggcc ccggcggccc
     5581 gggcggtggc ggcgcgggag gcccgggcgg cgcgggcgcg ggcgctgcgg cgggacagct
     5641 cgacgcgcag gttgggcccg aaggcatcct gcagaacggg gctgtggacg acagtgtagc
     5701 caagaccagc cagttgttgg ctgagttgaa cttcgaggaa gatgaagaag acacctatta
     5761 cacgaaggac ctccccatac acgcctgcag ttactgtgga atacacgatc ctgcctgcgt
     5821 ggtttactgt aataccagca agaagtggtt ctgcaacgga cgtggaaata cttctggcag
     5881 ccacattgta aatcaccttg tgagggcaaa atgcaaagag gtgaccctgc acaaggacgg
     5941 gcccctgggg gagacagtcc tggagtgcta caactgcggc tgtcgcaacg tcttcctcct
     6001 cggcttcatc ccggccaaag ctgactcagt ggtggtgctg ctgtgcaggc agccctgtgc
     6061 cagccagagc agcctcaagg acatcaactg ggacagctcg cagtggcagc cgctgatcca
     6121 ggaccgctgc ttcctgtcct ggctggtcaa gatcccctcc gagcaggagc agctgcgggc
     6181 acgccagatc acggcacagc agatcaacaa gctggaggag ctgtggaagg aaaacccttc
     6241 tgccacgctg gaggacctgg agaagccggg ggtggacgag gagccgcagc atgtcctcct
     6301 gcggtacgag gacgcctacc agtaccagaa catattcggg cccctggtca agctggaggc
     6361 cgactacgac aagaagctga aggagtccca gactcaagat aacatcactg tcaggtggga
     6421 cctgggcctt aacaagaaga gaatcgccta cttcactttg cccaagactg actctgacat
     6481 gcggctcatg cagggggatg agatatgcct gcggtacaaa ggggaccttg cgcccctgtg
     6541 gaaagggatc ggccacgtca tcaaggtccc tgataattat ggcgatgaga tcgccattga
     6601 gctgcggagc agcgtgggtg cacctgtgga ggtgactcac aacttccagg tggattttgt
     6661 gtggaagtcg acctcctttg acaggatgca gagcgcattg aaaacgtttg ccgtggatga
     6721 gacctcggtg tctggctaca tctaccacaa gctgttgggc cacgaggtgg aggacgtaat
     6781 catcaagtgc cagctgccca agcgcttcac ggcgcagggc ctccccgacc tcaaccactc
     6841 ccaggtttat gccgtgaaga ctgtgctgca aagaccactg agcctgatcc agggcccgcc
     6901 aggcacgggg aagacggtga cgtcggccac catcgtctac cacctggccc ggcaaggcaa
     6961 cgggccggtg ctggtgtgtg ctccgagcaa catcgccgtg gaccagctaa cggagaagat
     7021 ccaccagacg gggctaaagg tcgtgcgcct ctgcgccaag agccgtgagg ccatcgactc
     7081 cccggtgtct tttctggccc tgcacaacca gatcaggaac atggacagca tgcctgagct
     7141 gcagaagctg cagcagctga aagacgagac tggggagctg tcgtctgccg acgagaagcg
     7201 gtaccgggcc ttgaagcgca ccgcagagag agagctgctg atgaacgcag atgtcatctg
     7261 ctgcacatgt gtgggcgccg gtgacccgag gctggccaag atgcagttcc gctccatttt
     7321 aatcgccgca agcacccagg ccaccgagcc ggagtgcatg gttcccgtgg tcctcggggc
     7381 caagcagctg atccttgtag gcgaccactg ccagctgggc ccagtggtga tgtgcaagaa
     7441 ggcggccaag gccgggctgt cacagtcgct cttcgagcgc ctggtggtgc tgggcatccg
     7501 gcccatccgc ctgcaggtcc agtaccggat gcaccctgca ctcagcgcct tcccatccaa
     7561 catcttctac gagggctccc tccagaatgg tgtcactgca gcggatcgtg tgaagaaggg
     7621 atttgacttc cagtggcccc aacccgataa accgatgttc ttctacgtga cccagggcca
     7681 agaggagatt gccagctcgg gcacctccta cctgaacagg accgaggctg cgaacgtgga
     7741 gaagatcacc acgaagttgc tgaaggcagg cgccaagccg gaccagattg gcatcatcac
     7801 gccctacgag ggccagcgct cctacctggt gcagtacatg cagttcagcg gctccctgca
     7861 caccaagctc taccaggagg tggagatcgc cagtgtggac gcctttcagg gacgcgagaa
     7921 ggacttcatc atcctgtcct gtgtgcgggc caacgagcac caaggcattg gctttttaaa
     7981 tgaccccagg cgtctgaacg tggccctgac cagagcaagg tatggcgtca tcattgtggg
     8041 caacccgaag gcactatcaa agcagccgct ctggaaccac ctgctgaact actataagga
     8101 gcagaaggtg ctggtggagg ggccgctcaa caacctgcgt gagagcctca tgcagttcag
     8161 caagccacgg aagctggtca acactatcaa cccgggagcc cgcttcatga ccacagccat
     8221 gtatgatgcc cgggaggcca tcatcccagg ctccgtctat gatcggagca gccagggccg
     8281 gccttccagc atgtacttcc agacccatga ccagattggc atgatcagtg ccggccctag
     8341 ccacgtggct gccatgaaca ttcccatccc cttcaacctg gtcatgccac ccatgccacc
     8401 gcctggctat tttggacaag ccaacgggcc tgctgcaggg cgaggcaccc cgaaaggcaa
     8461 gactggtcgt gggggacgcc agaagaaccg ctttgggctt cctggaccca gccagactaa
     8521 cctccccaac agccaagcca gccaggatgt ggcgtcacag cccttctctc agggcgccct
     8581 gacgcagggc tacatctcca tgagccagcc ttcccagatg agccagcccg gcctctccca
     8641 gccggagctg tcccaggaca gttaccttgg tgacgagttt aaatcacaaa tcgacgtggc
     8701 gctcgcacag gactccacgt accagggaga gcgggcttac cagcatggcg gggtgacggg
     8761 gctgtcccag tattaaaagg tggcggcgga agagctaaag agtcgacccg ggcggccgct
     8821 tccctttagt gagggttaat gcttcgagca gacatgataa gatacattga tgagtttgga
     8881 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     8941 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     9001 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac
     9061 aaatgtggta aaatccgata aggatcgatc cgggctggcg taatagcgaa gaggcccgca
     9121 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg
     9181 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9241 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9301 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9361 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9421 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9481 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9541 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     9601 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9661 tcacaccgca tacgcggatc t
//
