LOCUS       sequence-362950-        9886 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Buganim Y, Itskovich E, Hu YC, Cheng AW, Ganz K, Sarkar S, Fu D, 
            Welstead GG, Page DC, Jaenisch R
  TITLE     Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like 
            Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2012 Sep 7;11(3):373-86.
  PUBMED    22958931
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2012-09-7"; volume: "11(3)"; pages: "373-86"
COMMENT     Sequence Label: FUW-TetO-Nr5a1
FEATURES             Location/Qualifiers
     source          1..9886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        172..551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        553..751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             769..949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    996..1121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1614..1847
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2032..2076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2374..2491
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2548..2566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2590..2608
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2632..2650
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2674..2692
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2716..2734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2809..2847
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2844..2868
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4512..5100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4565..4585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4983..4994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5103..5119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5104..5120)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5625..5805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5831..5848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5837..6061
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6107..6535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6194..6213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6404..6425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6544..6564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6549..6878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6729..6864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6791..6810
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6926..6973
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6992..7366
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7496..7617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7533..7552)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7587..7606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7666..7682)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7666..7682)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7679..7701)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7690..7706
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7714..7720,7721..7738,7739..7744))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7759..7780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7897..7914)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8068..8656)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8148..8167)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8827..9618,9619..9687))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9450..9469
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9688..9792)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9867..9886)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
       61 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
      121 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
      181 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      241 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      301 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      361 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      421 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      481 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      541 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      601 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      661 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      721 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
      781 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      841 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      901 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      961 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     1021 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1081 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1141 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1201 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1261 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1321 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1381 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1441 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1501 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1561 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1621 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1681 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1741 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1801 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1861 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1921 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1981 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     2041 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     2101 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     2161 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     2221 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     2281 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
     2341 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2401 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2461 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2521 agtttggtta gatctcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2581 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2641 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2701 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2761 ctatcagtga tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta
     2821 cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     2881 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc
     2941 gaattcgccc tttccagtgt ccacccttat ccggctgaga attctccttc cgttcagcgg
     3001 acgccgcggg catggactat tcgtacgacg aggacctgga cgagctgtgt ccagtgtgtg
     3061 gtgacaaggt gtcgggctac cactacgggc tgctcacgtg cgagagctgc aagggcttct
     3121 tcaagcgcac agtccagaac aacaagcatt acacgtgcac cgagagtcag agctgcaaaa
     3181 tcgacaagac gcagcgtaag cgctgtccct tctgccgctt ccagaagtgc ctgacggtgg
     3241 gcatgcgcct ggaagctgtg cgtgctgatc gaatgcgggg tggccggaac aagtttgggc
     3301 ccatgtacaa gagagaccgg gccttgaagc agcagaagaa agcacagatt cgggccaatg
     3361 gcttcaagct ggagaccgga ccaccgatgg gggtgccccc gccaccccct cccccaccgg
     3421 actacatgtt accccctagc ctgcacgcac cggagcccaa ggccctggtc tctggcccac
     3481 ccagtgggcc gctgggtgac tttggagccc catctctacc catggctgtg cctggtcccc
     3541 acggacctct ggctggctac ctctatcctg ccttctctaa ccgcaccatc aagtctgagt
     3601 atccagagcc ctatgccagc cccccacaac agccagggcc accctacagc tatccagagc
     3661 ccttctcagg agggcccaat gtaccagagc tcatattgca gctgctgcaa ctagagccag
     3721 aggaggacca ggtgcgcgct cgcatcgtgg gctgtctgca ggagccagcc aaaagccgct
     3781 ctgaccagcc agcgcccttc agcctcctct gcagaatggc cgaccagacc tttatctcca
     3841 ttgtcgactg ggcacgaagg tgcatggtct ttaaggagct ggaggtggct gaccagatga
     3901 cactgctgca gaactgttgg agcgagctgc tggtgttgga ccacatctac cgccaggtcc
     3961 agtacggcaa ggaagacagc atcctgctgg ttactggaca ggaggtggag ctgagcacag
     4021 tggctgtgca ggctggctcc ctgctgcaca gcctggtgct gcgggcccaa gagttagtgc
     4081 tccagttgca tgcactgcag ctggaccgcc aggagttcgt ctgtctcaag ttcctcatcc
     4141 tcttcagcct cgatgtgaaa ttcctgaaca accacagcct cgtaaaggac gcccaggaaa
     4201 aggccaacgc tgccctgttg gattacacct tgtgtcacta cccacactgc ggggacaaat
     4261 tccagcagtt gctattgtgc ctggtggagg tgcgggccct gagcatgcag gccaaggagt
     4321 acctgtacca caagcatttg ggcaacgaga tgccccgcaa caaccttctc attgagatgc
     4381 tgcaggccaa gcagacttga gcctgggtgc caggcagcgg gcaataggca gggatgccac
     4441 tgcctccaaa agactccttg cattaggtga tccaggagcc caagggcgaa ttcgatatca
     4501 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4561 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4621 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4681 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     4741 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     4801 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4861 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4921 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4981 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5041 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5101 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     5161 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     5221 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5281 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     5341 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     5401 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     5461 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     5521 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     5581 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     5641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     5821 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5881 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5941 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6001 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6061 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6121 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6181 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6241 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6301 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6361 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6421 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6481 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6541 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6601 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6661 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6781 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6841 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6901 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     6961 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     7021 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     7081 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     7141 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     7201 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     7261 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     7321 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     7381 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7441 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7621 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     7681 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     7741 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     7801 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     7861 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     7921 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7981 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8041 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8101 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8161 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8221 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8281 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8341 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8401 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8461 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8521 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8581 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8641 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     8701 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     8761 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     8821 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     8881 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     8941 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9001 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9061 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9121 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9181 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9241 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9301 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9361 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9421 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9481 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9541 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9601 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     9661 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     9721 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     9781 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     9841 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccg
//
