LOCUS       sequence-363165-        3131 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pSwap plasmid part with HB homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Teufel M, Klein CA, Mager M, Sobetzko P
  TITLE     A multifunctional system for genome editing and large-scale 
            interspecies gene transfer.
  JOURNAL   Nat Commun. 2022 Jun 14;13(1):3430. doi: 10.1038/s41467-022-30843-1.
  PUBMED    35701417
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-06-14"; volume: "13(1):3430. doi"; pages: " 
            10.1038/s41467-022-30843-1"
COMMENT     Sequence Label: pSWAP_Entry_HB
FEATURES             Location/Qualifiers
     source          1..3131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    98..119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(134..139,140..157,158..164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    172..188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     177..199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     196..212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     196..212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             208..531
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    225..281
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(282..299)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(282..298)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(291..313)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(507..526)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             873..1178
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1057..1076
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(1337..1355)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1423..1527
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1528..2322
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(2382..2970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2462..2481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtctggacga agacagcgct ccgttgagac ccagtggtat gggcagctgg cacgacaggt
       61 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
      121 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
      181 gataacaatt tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc
      241 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa
      301 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      361 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      421 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
      481 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gtcgcgtgga
      541 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
      601 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
      661 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
      721 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
      781 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
      841 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
      901 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
      961 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     1021 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     1081 gtgtgcctgt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     1141 tcaaaaacgc cattaacctg atgttctggg gaatataatt aagccagccc cgacacccgc
     1201 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     1261 ctgtgaccca ctcaagtggg tctcacgctc cgtcaccgtt tggaccttgg ttcagtggtc
     1321 tgtcttcgac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     1381 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     1441 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     1501 cttcaataat attgaaaaag gaagagtatg gctaaaatga gaatatcacc ggaattgaaa
     1561 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
     1621 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
     1681 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
     1741 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctaatgagt
     1801 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
     1861 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
     1921 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
     1981 gccgatgtgg attgcgaaaa ctgggaagag gacactccat ttaaagatcc gcgcgagctg
     2041 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
     2101 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
     2161 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgct cagggaggat
     2221 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
     2281 gagaaaataa aatattatat tttactggat gaattgtttt agctgtcaga ccaagtttac
     2341 tccggggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2401 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2461 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2521 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2581 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2641 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2701 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2761 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2821 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2881 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2941 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3001 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3061 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3121 gagtaaactt g
//
