LOCUS       sequence-363639-        9525 bp    DNA     circular SYN 15-APR-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(FCA) with an N-terminal NLS and C-terminal NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(FCA)-6xHis-NLS(SV40)
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..69)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        241..620
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        621..824
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     775..795
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     869..888
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        869..887
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4957..4974
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4993..5013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5122..5139)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5128..5352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5398..5826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5485..5504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5695..5716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5835..5855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5840..6169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6020..6155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6082..6101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6236..7030
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6290..6309)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6900..6919
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7204..7325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7241..7260)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7295..7314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7374..7390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7374..7390)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7387..7409)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7398..7414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7422..7428,7429..7446,7447..7452))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7467..7488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7605..7622)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7776..8364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7856..7875)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8535..9326,9327..9395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9158..9177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9396..9500)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
       61 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
      121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
      181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
      241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      841 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      901 gctagcatgc ccaagaaaaa gcggaaggtg cccaagatga agaaggacta cagcatcggc
      961 ctggacatcg gcaccaattc tgttggctgg gccgtgaccg acgacaacta caacctcgtg
     1021 cggaagaaaa tgaaggtgct gggcaacacc gacaagaagt ccatcggaaa ggccctgtgg
     1081 ggcgtcagac tgtttgatgc cgctgagaca gccgaggaac ggcggatgca cagaaccacc
     1141 agaaggcggt acacccggcg gagacagaga atcgatctgc tgcaagagat cttccaagag
     1201 gaaatcagca aggtcgaccc cagcttcttt atccggctga acgagagcag actgcacccc
     1261 gaggacaaga ccgacgatag acaccctctg ttcggcgaca acgagacaga caaggactac
     1321 cacaagcagt accccaccat ctaccacctg agaaagcacc tgatggaaag cgacgagaag
     1381 cacgacatcc ggctggtgta tctggccctg caccacatca tcaagtaccg gggccacttc
     1441 ctgatcgagg gcgacctgaa tagcgagaac accgacgtgg aagaactgtt caagcagctg
     1501 gtccaggtgt tcaacgacac cttcgaggaa gaacacctga gcgaggaagc catcgacatc
     1561 gaggaaatcc tgacggataa gaagtcccgg tccaccagag ccaaagaggt ggtcaagctg
     1621 ttcggcagcg agaagaagca ggcctgtatc agcgccctga tcaagctgat cgtgggcctg
     1681 aagggcaacc tgaagaaggt gttcggggac gccgaggaca ccagcatcca cttcagcaag
     1741 gacaactacg aagaggacct ggaagccatt cgggacatca tccccgacga gtacgccgat
     1801 ctgttcgagg ccatcaaagc cctgtacgac gccatcgtgc tgtctggcat tctcggcggc
     1861 agcacctcta acaccaaggc caaagtgtcc gccagcatga tcgccagata cgagcagcac
     1921 cagaaagacc tgaagcagct gaaacagttc gtgaaagagc atctgcccga gaagtacaac
     1981 gagatcttca gcgacaacac caagaacggc tacagcgcct acatcgaagg cggcaccagc
     2041 caagaggact tctacaagta tctgaagaag atcctgaaag agctggaaga ggccgagtac
     2101 ctgctggaag aaatcgagaa cgagaacttc ctgcctaagc agcggacctc cgacaacggc
     2161 gtgatccctt atcagatcca cctcgaggaa ctgcgggcca tcctgaagaa ccagggcaag
     2221 tactacccat tcctcaaaga gaacgccgag aagatcgagt ctatcctgac cttcagaatc
     2281 ccctactacg tgggaccact ggccagaggc aatggcagat tcgcctggat gatcagaaag
     2341 aaggacggca agatcacccc ttggaacttc gacgaggtgg tggacaaaga gaagtccgcc
     2401 gaggaattca tcgagcggat gaccaacaac tgcacctacc tgcctggcga gaacgtgctg
     2461 ccaaagaaca gcctgctgta cgagaagttc accgtgctga atgagctgaa caacgtgcgg
     2521 ctgaccaccg ataagggcaa gaccagaaga ttcagcgccg agcagaagca agagattttc
     2581 gacgacctgt tccggaagaa ccggaaagtg accaagaaga agctcgagga ctacctgaag
     2641 cgcgagtacg aagagttcga ctccgccgac atctccggca tcgatggcga gtttaagagc
     2701 agcctgggca gctaccacga cttctgcaag atcgtggtca agggcaacag cctggacgag
     2761 gaagattaca aggatatcat cgaagagatc attaagtggc tgaccgtgtt cgaggaccgg
     2821 aagatgctgc ggagaaggct ggaaaagtac tccgagatcc tgaccgagga acagatcaag
     2881 aagatctgca agcggcacta caccggctgg ggcagactgt ctagaaagct gctgaccggc
     2941 atccgggaca aagaaaccgg caagagcatc atcgacgtgc tgcgggaaac cgacagcagc
     3001 aaccggaact tcatgcagct gctgagcgac gaggacctga gcttcaaaga agagatcgag
     3061 caggccaacg ccgaggccga aggcgaaaat ctgcacgaga tcgtcgagga tctgcctggc
     3121 tctcccgcca tcaagagagg aattctgcag gccctgaaga ttgtggatga gatcgtgaaa
     3181 gtgatggggc acgagcccaa gaacatcttc gtggaaatgg cccgcgagaa ccagaaaacc
     3241 ggcagaggca gacgctccac caagaaaaga ctgaagcgcc tgcaagaggc cctcaagaac
     3301 ctgggctcca acctgctcaa agagctgccc cgggacgata acgagctgcg gaacgataga
     3361 ctgttcctgt actacaccca gatgggcaag tgcatgtaca ccggcgagcc catcgatctg
     3421 gacgacctga gcaactacga catcgatcac atcattcccc agagcttcat caaggacgac
     3481 tccctggaca accgggtgct cgtgtccaga gaggaaaacg cccgcaagac cgataacttc
     3541 ccatctcctg agatccggcg gaagatgaag tccttctggc aaatgctgct gaaggccgga
     3601 ctgatctcta agaagaagtt tgacagactg accagagccg acagaggcga cttcaccgat
     3661 gatgagctgg ccggctttat tgcccggcag ctggtggaaa cccggcagat cacaaaacac
     3721 gtggccacac tgctgaagca gagatacccc accgagaagg atgaggaaga taagaccatc
     3781 cggaacgcca agattgtgtc cgtgaaggcc aatctggtgt ccgagttcag acaggacttc
     3841 ggcctgtaca agtgccggga agtgaacgac taccatcacg cccacgacgc ctacctgaat
     3901 gccgttgtgg gaaacgccct gctgaagaag taccctcagc tggccgccga gtttatcaag
     3961 ggcgactaca gaaagaacaa cgcccgggaa gagaacaagg ccaatgccaa gatgcacttt
     4021 tacagcaaca tcatgaacag cttcacctcc gacgtgaaga tcgccgacga gacaggcgag
     4081 atcgtgtggg acaaagagaa agatatcgcc accgtgcgga aagtcatgaa ctaccaccag
     4141 gtgctgatca cccgcaaggt ggaagaggaa aaaggcggct tcttcgacca gaccatcctg
     4201 agcaagggca atagcaagaa gctgatcccg ctgaagaaaa atctggaccc tgagaagtac
     4261 ggcggctaca actctcctac cgtggcctac tctgtgctgg tcgagtacga tatcgagaag
     4321 ggcaaaaaga aaaaactcaa gaccgttaag cagctcgtgg gcatccccat cagagagaga
     4381 gccaagctgg aaaaaaaccc catcaagtat ctcgagaaaa agggctatca gaaccctaag
     4441 gtggacctgc tgatcaagat ccccaagaac tccctgttcg agctggacgg cggcaggcga
     4501 agaatactgg ccgctgcaaa agagctgaaa aacgccaatc agctggtgct ccccgccgaa
     4561 gagtataccc tgctggataa ggtggccaaa atcattaaga agaacaacag cgagagcatc
     4621 gagtacgtcg aggaacacct gtctgagttc gatgagctgc tggaaagcct gatcgactac
     4681 agccctaagc tggctctgca ggacaagaat ctggaaaaga tcaaagaggc cttcgaacag
     4741 ctgaacctgg ccgataagaa agaagtggcc aaagagatta tcaacctgct gcactgcacc
     4801 gccaccaccg ctaatgccgc tctgaagttt ctcggaggct ccaagaacag aatgcgctac
     4861 acctccatca aagaactgct gaacgccagc ctgatccacc agtctatcac cggcctgtat
     4921 gagacacgga tcgacctggg aaagctgggc gaagatcacc atcatcatca ccacgcctct
     4981 ggaacaggcg gacctaaaaa gaagaggaag gtttaaggta ccgagctcgg atccactagt
     5041 ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     5101 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5161 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5221 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5281 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5341 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5821 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     5881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     5941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6181 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6241 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6301 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6361 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     6421 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     6481 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     6541 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     6601 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     6661 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6721 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     6781 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6841 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6901 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6961 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7021 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     7081 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7141 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7201 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7321 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7381 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7441 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7501 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7561 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7621 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7681 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7741 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8461 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8521 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8581 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8641 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8701 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8761 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8821 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8881 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8941 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9001 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9061 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9121 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9181 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9241 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9301 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9361 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9421 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9481 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacct
//
