LOCUS       sequence-363837-        8815 bp    DNA     circular SYN 15-APR-2025
DEFINITION  For tetracycline-inducible mammalian expression of shRNA: 
            GCCGTGACCAGATTATCTTTA that targets human ARAF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  TITLE     Najafov lab plasmids
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO-Tet-puro-hARAF-shRNA-1
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     42..59
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    176..197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(212..217,218..235,236..242)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    250..266
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     255..277
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     274..290
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     274..290
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     309..329
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        311..329
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        357..583
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             584..764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1429..1662
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1847..1891
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2180..2274
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     2191..2210
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    2228..2246
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2256..2274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    2374..2491
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2540..3050
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2969..2987
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          3059..3631
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3639..3658
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3686..3704
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3715..4335
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3788..3807)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    4370..4943
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4537..4554)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4764..4783
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4963..5562
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4963..4982)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5459..5479
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5690..5923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5995..6116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6032..6051)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6086..6105
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6156..6291
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(6312..6330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6340..6357)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6340..6356)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6349..6371)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6498..6953
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6585..6604)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6795..6816
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6979..7083
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7084..7152,7153..7944)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7302..7321)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8115..8703
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8604..8623
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
       61 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      121 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      181 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      241 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      301 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
      361 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
      421 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
      481 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
      541 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     1321 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     1381 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     1441 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     1501 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     1561 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     1621 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     1681 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     1741 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     1801 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     1861 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     1921 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     1981 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     2041 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
     2101 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga
     2161 ctagcctcga gcggccgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa
     2221 cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata gagacaccgg
     2281 gccgtgacca gattatcttt actcgagtaa agataatctg gtcacggctt tttaattctc
     2341 gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     2401 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     2461 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     2521 actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg
     2581 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     2641 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     2701 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     2761 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     2821 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2881 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2941 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     3001 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatctgt
     3061 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     3121 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     3181 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     3241 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     3301 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     3361 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     3421 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     3481 tattggtaga aacaactaca tcctggtcat catcctgcct ttctctttat ggttacaatg
     3541 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     3601 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     3661 gtctcatcat tttggcaaag aattgtaata cgactcacta tagggcgaat tgatatgtct
     3721 agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc
     3781 gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat
     3841 tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg
     3901 caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac
     3961 gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt
     4021 acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa
     4081 caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta
     4141 ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact
     4201 actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca
     4261 gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa
     4321 tgtgaaagtg ggtccgcgta cagcggctcc cgggagttct agggatctgc ccctctccct
     4381 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     4441 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     4501 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     4561 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     4621 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     4681 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     4741 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     4801 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     4861 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     4921 tcctttgaaa aacacgatga taaggatcca ccggagctta ccatgaccga gtacaagccc
     4981 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     5041 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     5101 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     5161 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5221 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5281 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5341 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5401 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5461 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     5521 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     5581 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     5641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     5701 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     5761 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     5821 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     5881 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     5941 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     6001 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6061 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6121 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6181 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6241 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     6301 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     6361 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6421 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6481 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6541 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6601 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6661 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6721 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6781 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6841 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6901 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     6961 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7021 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7081 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7141 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7201 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7261 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7321 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7381 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7441 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7501 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7561 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7621 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     7681 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     7741 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     7801 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     7861 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7921 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7981 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8041 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8101 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8161 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8221 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8281 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8341 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8401 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8461 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8521 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8581 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8641 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8701 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8761 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttg
//
