LOCUS       sequence-364136-       10827 bp    DNA     circular SYN 14-APR-2025
DEFINITION  CRISPR-Cas12a genome editing of E. faecium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Chua MJ, Collins J
  TITLE     Rapid, Efficient, and Cost-Effective Gene Editing of Enterococcus 
            faecium with CRISPR-Cas12a.
  JOURNAL   Microbiol Spectr. 2022 Feb 2:e0242721. doi: 
            10.1128/spectrum.02427-21.
  PUBMED    35107356
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Spectr. 2022 Feb 2:e0242721. doi: 10.1128/spectrum.02427-21."
COMMENT     Sequence Label: pJC005.gent
FEATURES             Location/Qualifiers
     source          1..10827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             293..826
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_RNA        complement(4368..4446)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     rep_origin      complement(4569..5157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4649..4668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5474..5490
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5474..5490
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(5557..6183)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     6088..6107
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     protein_bind    6290..6308
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             6373..10293
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     primer_bind     complement(10544..10561)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10544..10560)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10553..10575)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gaattactat cccttttatc aagaagcgca caaaaagaaa aacgaaatga tacaccaatc
       61 agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga cttgcaccat
      121 atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat ggaaataaga
      181 cttagaagca aacttaagag tgtgttgata gtgcagtatc ttaaaatttt gtataatagg
      241 aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagtgatt acatgttacg
      301 cagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttag gtggctcaag
      361 tatgggcatc attcgcacat gtaggctcgg ccctgaccaa gtcaaatcca tgcgggctgc
      421 tcttgatctt ttcggtcgtg agttcggaga cgtagccacc tactcccaac atcagccgga
      481 ctccgattac ctcgggaact tgctccgtag taagacattc atcgcgcttg ctgccttcga
      541 ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg cccaagtttg agcagccgcg
      601 tagtgagatc tatatctatg atctcgcagt ctccggcgag caccggaggc agggcattgc
      661 caccgcgctc atcaatctcc tcaagcatga ggccaacgcg cttggtgctt atgtgatcta
      721 cgtgcaagca gattacggtg acgatcccgc agtggctctc tatacaaagt tgggcatacg
      781 ggaagaagtg atgcactttg atatcgaccc aagtaccgcc acctaattct atgagtcgct
      841 tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta ttaggtatac
      901 tactgacagc ttccaaggag ctaaagaggt ccctagcgct tagaatcgct ttaggaaaca
      961 cgatccagtc caataatcgt cgataaaaac ttttgaaaaa ggttggtgaa attacctact
     1021 tttggaataa tcacaaatca caagtgatta atcacaaatc acaagtgatt aatcacttgt
     1081 ttattaagat attaaaagct ataatttaaa taaagcgtga attttattac acaaaaagag
     1141 gggggagaaa cttggaacta gcatttagag aaagcttaaa aaagatgaga ggtaccaaat
     1201 caaaagaaaa attctcccaa gaattagaaa tgagtagatc aaattattca cgaatagaat
     1261 caggaaaatc agatccaacc ataaaaacac cagaacaaat tgcaaagtta actaactcaa
     1321 cgctagtagt ggatttaatc ccaaatgagc caacagaacc agaaccagaa acagaatcag
     1381 aacaagtaac attggattta gaaatggaag aagaaaaaag caatgacttc gtgtgaataa
     1441 tgcgcgaaat cgttgcttat tttttttaaa agcggtatac tagatataac gaaacaacga
     1501 actgaataga aacgaaaaaa gagccatgac acatttataa aatgtttgac gacattttat
     1561 aaatgcatag cccgataaga ttgccaaacc aacgcttatc agttagtcag atgaactctt
     1621 ccctcgtaag aagttattta attaactttg tttgaagacg gtatataacc gtactatcat
     1681 tatataggga aatcagagag ttttcaagta tctaagctac tgaatttaag aattgttaag
     1741 caatcaatcg gaaatcgttt gattgctttt tttgtattca tttatagaag gtggagtttg
     1801 tatgaatcat gatgaatgta aaacttatat aaaaaatagt ttattggaga taagaaaatt
     1861 agcaaatatc tatacactag aaacgtttaa gaaagagtta gaaaagagaa atatctactt
     1921 agaaacaaaa tcagataagt atttttcttc ggagggggaa gattatatat ataagttaat
     1981 agaaaataac aaaataattt attcgattag tggaaaaaaa ttgacttata aaggaaaaaa
     2041 atctttttca aaacatgcaa tattgaaaca gttgaatgaa aaagcaaacc aagttaatta
     2101 aacaacctat tttataggat ttataggaaa ggagaacagc tgaatgaata tcccttttgt
     2161 tgtagaaact gtgcttcatg acggcttgtt aaagtacaaa tttaaaaata gtaaaattcg
     2221 ctcaatcact accaagccag gtaaaagcaa aggggctatt tttgcgtatc gctcaaaatc
     2281 aagcatgatt ggcggtcgtg gtgttgttct gacttccgag gaagcgattc aagaaaatca
     2341 agatacattt acacattgga cacccaacgt ttatcgttat ggaacgtatg cagacgaaaa
     2401 ccgttcatac acgaaaggac attctgaaaa caatttaaga caaatcaata ccttctttat
     2461 tgattttgat attcacacgg caaaagaaac tatttcagca agcgatattt taacaaccgc
     2521 tattgattta ggttttatgc ctactatgat tatcaaatct gataaaggtt atcaagcata
     2581 ttttgtttta gaaacgccag tctatgtgac ttcaaaatca gaatttaaat ctgtcaaagc
     2641 agccaaaata atttcgcaaa atatccgaga atattttgga aagtctttgc cagttgatct
     2701 aacgtgtaat cattttggta ttgctcgcat accaagaacg gacaatgtag aattttttga
     2761 tcctaattac cgttattctt tcaaagaatg gcaagattgg tctttcaaac aaacagataa
     2821 taagggcttt actcgttcaa gtctaacggt tttaagcggt acagaaggca aaaaacaagt
     2881 agatgaaccc tggtttaatc tcttattgca cgaaacgaaa ttttcaggag aaaagggttt
     2941 aatagggcgt aataacgtca tgtttaccct ctctttagcc tactttagtt caggctattc
     3001 aatcgaaacg tgcgaatata atatgtttga gtttaataat cgattagatc aacccttaga
     3061 agaaaaagaa gtaatcaaaa ttgttagaag tgcctattca gaaaactatc aaggggctaa
     3121 tagggaatac attaccattc tttgcaaagc ttgggtatca agtgatttaa ccagtaaaga
     3181 tttatttgtc cgtcaagggt ggtttaaatt caagaaaaaa agaagcgaac gtcaacgtgt
     3241 tcatttgtca gaatggaaag aagatttaat ggcttatatt agcgaaaaat cagatgtata
     3301 caagccttat ttagtgacga ccaaaaaaga gattagagaa gtgctaggca ttcctgaacg
     3361 gacattagat aaattgctga aggtactgaa ggcgaatcag gaaattttct ttaagattaa
     3421 accaggaaga aatggtggca ttcaacttgc tagtgttaaa tcattgttgc tatcgatcat
     3481 taaagtaaaa aaagaagaaa aagaaagcta tataaaggcg ctgacaaatt cttttgactt
     3541 agagcataca ttcattcaag agactttaaa caagctagca gaacgcccta aaacggacac
     3601 acaactcgat ttgtttagct atgatacagg ctgaaaataa aacccgcact atgccattac
     3661 atttatatct atgatacgtg tttgtttttt ctttgctgtt tagcgaatga ttagcagaaa
     3721 tatacagagt aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact
     3781 tttagttggc ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg
     3841 agagcgaagc gaacacttga ttttttaatt ttctatcttt tataggtcat tagagtatac
     3901 ttatttgtcc tataaactat ttagcagcat aatagattta ttgaataggt catttaagtt
     3961 gagcatatta gaggaggaaa atcttggaga aatatttgaa gaacccgatt acatggattg
     4021 gattagttct tgtggttacg tggtttttaa ctaaaagtag tgaatttttg atttttggtg
     4081 tgtgtgtctt gttgtcagta tttgctagtc aaagtgatta aatagaattc tcatgtttga
     4141 cagcttatca tcggagctcc gatgataagc tgtcaaacat gagaattccc ggggatcctt
     4201 acgaaatcat cctgtggagc ttagtaggtt tagcaagatg gcagcgccta aatgtagaat
     4261 gataaaagga ttaagagatt aatttcccta aaaatgataa aacaagcgtt ttgaaagcgc
     4321 ttgttttttt ggtttgcagt cagagtagaa tagaagtatc aaaaaaagca ccgactcggt
     4381 gccacttttt caagttgata acggactagc cttattttaa cttgctatgc tgttaagaat
     4441 ggttccaacg ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4981 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5281 agttttaaat caatctaaag tatatatgag taaagatgtg atccgtagcg gtatgcgctc
     5341 catcaagaag agcgacttcg cggagctggt gaagtacatc accgacgagc aaggcaagac
     5401 cgatcgggcc ccctgcagga taaaaaaatt gtagataaat tttataaaat agttttatct
     5461 acaatttttt tatcaggaaa cagctatgac cgcggccgct gtatccacat aaaaataaga
     5521 agcctgcatt tgcaggcttc ttatttttat ggtaccttaa gacccacttt cacatttaag
     5581 ttgtttttct aatccgcata tgatcaattc aaggccgaat aagaaggctg gctctgcacc
     5641 ttggtgatca aataattcga tagcttgtcg taataatggc ggcatactat cagtagtagg
     5701 tgtttccctt tcttctttag cgacttgatg ctcttgatct tccaatacgc aacctaaagt
     5761 aaaatgcccc acagcgctga gtgcatataa tgcattctct agtgaaaaac cttgttggca
     5821 taaaaaggct aattgatttt cgagagtttc atactgtttt tctgtaggcc gtgtacctaa
     5881 atgtactttt gctccatctc ggtgacttag taaagcacat ctaaaacttt tagcgttatt
     5941 acgtaaaaaa tcttgccagc tttccccttc taaagggcaa aagtgagtat ggtgcctatc
     6001 taacatctca atggctaagg cgtcgagcaa agcccgctta ttttttacat gccaatacaa
     6061 tgtaggctgc tctacaccta gcttctgggc gagtttacgg gttgttaaac cttcgattcc
     6121 gacctcatta agcagctcta atgcgctgtt aatcacttta cttttatcta atctagacat
     6181 cattaattcc tcctttttgt tgacattata tcattgatag agttatttgt caaactagtt
     6241 ttttatttcg atgccctgga cttcatgaaa aactaaaaaa aatattgaca ctctatcatt
     6301 gatagagtat aattaaaata agcttgatcg tagcgttaac agatctgagc tcctgcagta
     6361 aaggaggttt ttatgacaca gtttgaaggc ttcaccaatc tctaccaggt cagcaagacg
     6421 ctacgttttg agcttatccc gcagggaaaa accctgaaac acattcagga acaggggttc
     6481 atagaggaag ataaggcgcg taacgaccat tataaagaac tgaagcctat aatcgaccgt
     6541 atttataaaa cgtacgcgga tcagtgcctg cagctggttc agctggattg ggagaatctg
     6601 tccgcggcta ttgatagcta tcgcaaagag aagaccgagg aaacccgtaa cgcactgatt
     6661 gaagagcagg cgacctatcg gaatgcgatc catgattact tcatcggccg caccgacaac
     6721 ctgaccgatg caattaacaa acgtcacgca gagatttaca aaggtctgtt taaagcagag
     6781 ttattcaatg gcaaggttct gaaacagctg ggtacggtca ccaccaccga acacgaaaac
     6841 gcactgctga ggagctttga taaatttacc acatatttca gcggtttcta tgaaaatcgt
     6901 aagaatgtat ttagcgccga agatatttcc accgcaattc ctcatcgtat tgtgcaggat
     6961 aattttccga agtttaaaga aaattgtcat atttttaccc gtctgatcac cgcggtaccg
     7021 agcctgcgag agcattttga aaacgttaag aaagccattg gaatttttgt cagtaccagc
     7081 attgaagaag tgttttcgtt cccgttctat aaccaactgc tgacccagac ccagattgat
     7141 ctgtacaatc agctgctggg gggcataagc cgcgaggcag gtaccgaaaa gataaaggga
     7201 ctcaatgagg tgctgaatct ggcaattcag aagaatgatg aaacggctca tatcattgct
     7261 agcctgccgc atcgtttcat tcccctgttt aagcaaatcc tgagcgatcg caatacactg
     7321 agctttatcc tcgaagagtt taaatcggac gaagaagtta tccagagctt ttgcaaatac
     7381 aaaaccctgc tgcggaacga aaatgtgctg gagaccgctg aagcactgtt taatgaactg
     7441 aactcgatcg acctcaccca tatttttata tcccacaaaa aactggaaac cataagcagc
     7501 gctctgtgtg accattggga taccctgcgc aacgccctgt atgaacggcg tatcagcgag
     7561 ctgaccggga aaatcaccaa atccgcaaag gaaaaagttc agcgtagtct gaaacacgag
     7621 gacatcaacc tgcaagaaat tattagcgca gcaggtaaag agctgagcga agcattcaaa
     7681 cagaaaacca gcgaaatcct gagccatgcc catgctgcac tggatcagcc gctgccgacc
     7741 accctgaaaa aacaggagga aaaggagatt ctgaaaagcc aactggacag cctgctgggc
     7801 ctgtatcacc tgctggactg gtttgcagtc gatgagagca acgaggttga tcctgagttc
     7861 tccgctcgtc tgaccggaat caagctggag atggaaccga gtctgtcgtt ttacaataaa
     7921 gcgcgtaatt acgcgaccaa gaaaccgtat agcgtggaaa aattcaaact gaactttcag
     7981 atgccgaccc ttgcaagcgg atgggacgtt aacaaagaaa aaaacaatgg ggcaattctg
     8041 tttgtgaaaa atggcctcta ttatctgggt atcatgccga aacagaaagg gcgctacaaa
     8101 gccctgtcat ttgagccgac cgagaaaacc tcagagggtt tcgacaagat gtactacgat
     8161 tatttcccgg atgcggcaaa aatgataccc aaatgtagca cccaactgaa ggcagttaca
     8221 gcccactttc agacccatac caccccgatc ctgctgtcga acaattttat agagccgctg
     8281 gaaattacca aagagattta tgatctgaat aatccggaaa aggagcccaa gaaatttcag
     8341 acggcgtatg caaaaaagac cggggatcag aaaggttatc gtgaagcgct gtgcaaatgg
     8401 attgacttta cccgtgactt tctgtcaaaa tataccaaaa cgacgagcat tgatctgagc
     8461 agcctacgtc cgagcagcca atataaggat ctgggcgaat attacgccga actgaatccg
     8521 ctgctctacc atatttcctt ccaacgaatc gctgaaaaag aaataatgga cgccgttgaa
     8581 accggcaaac tgtatctgtt tcaaatctac aacaaagatt tcgccaaagg ccatcacggt
     8641 aagccgaacc tgcataccct gtattggacc ggtctgttta gcccggagaa tctggccaaa
     8701 accagcatca agctgaacgg acaggcagaa ctgttttacc gccccaaaag ccgtatgaaa
     8761 aggatggcac accgcctggg cgaaaaaatg ctgaataaga aactcaaaga tcagaaaacg
     8821 ccgataccgg atacccttta tcaggagctg tatgattatg ttaaccaccg gctgagccat
     8881 gacctgagcg acgaagcgcg tgcactgctg ccgaacgtga ttaccaagga agtctcgcat
     8941 gaaattatta aagatcggcg cttcaccagt gataaatttt tcttccatgt accgatcacc
     9001 ctgaattatc aagccgcaaa tagcccttcc aaatttaatc aacgcgtgaa tgcgtacctg
     9061 aaagagcatc cggagacccc aattattggc atagaccgag gagaacgcaa tctcatttat
     9121 atcaccgtca ttgatagcac cggtaagatc ctggaacagc gtagcctgaa taccattcag
     9181 cagtttgact accagaaaaa gctggacaac agagaaaagg aacgtgtagc cgcccggcag
     9241 gcttggagtg tggtgggtac tatcaaggat ctgaagcagg ggtatctctc ccaagttatc
     9301 catgaaattg tcgatctaat gattcactat caagcagtag tggtactgga aaatctgaat
     9361 ttcggtttca aaagcaaacg tacagggatc gctgaaaaag ccgtttatca gcagttcgag
     9421 aaaatgctga tagacaagct gaattgcctg gttctgaaag attatccggc agagaaggtg
     9481 ggcggtgtgc tgaacccgta ccagctgact gatcaattta cgagctttgc aaaaatggga
     9541 acgcagagcg gtttcctgtt ctatgttccg gcgccatata ccagcaagat agacccgctg
     9601 acaggtttcg tagatccgtt tgtctggaaa accattaaaa atcatgaaag tcgcaaacat
     9661 tttctggagg gctttgattt tctgcactat gacgtgaaaa ccggcgactt cattctgcat
     9721 tttaaaatga accgtaatct gtcctttcag cgcggcctgc ctggctttat gccggcgtgg
     9781 gacattgttt ttgaaaagaa tgagacacag tttgatgcca aaggtacccc ctttattgcg
     9841 gggaaacgca ttgtgcccgt tatagaaaat caccgcttca ccggacggta tagggacttg
     9901 tacccggcaa atgaattgat agcgctgctg gaggagaaag gtattgtctt tcgggatgga
     9961 tcaaacatcc tgccgaagct gctggagaac gatgacagcc acgcaataga caccatggta
    10021 gcgctgatcc gaagcgtgct gcagatgcgt aacagtaatg cggctacggg ggaagactac
    10081 attaatagcc cggtccgtga tctgaacggc gtttgtttcg atagcagatt tcaaaatccg
    10141 gagtggccga tggatgccga tgccaatgga gcttaccata tcgctctcaa aggtcagctc
    10201 ctactgaacc atttgaaaga atcaaaagat ctgaaactgc agaacggcat ctcgaatcag
    10261 gactggctgg cctacattca agaactgaga aactaatatg accatgatta cgaattcgag
    10321 ctcggtaccc ggggatccta taagttttaa taaaacttta aatagaaaaa ggcttctctc
    10381 atgagaagtc ttttttattt aaaataaata taaaataaaa tagaggctat aaatagcctc
    10441 tattttatgt gagaaatccc taaataaaaa gatgccagtg tgctggaatt cgtcatcgca
    10501 tgagtcagcg atgactcgag gcctgcagac atgcaagctt ggcactggcc gtcgttttac
    10561 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
    10621 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
    10681 gcagcctgaa tggcgaatgg cgctagcata aaaataagaa gcctgcattt gcaggcttct
    10741 tatttttatg gcgcgccgtt ctgaatcctt agctaatggt tcaacaggta actatgacga
    10801 agatagcacc ctggagcaac caggaat
//
