LOCUS       sequence-364340-        3834 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor Vector containing ZNF830 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF830
FEATURES             Location/Qualifiers
     source          1..3834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1145..1244)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1261..1280)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1262..1280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1285..1301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1285..1301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1732..2523
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2616..3204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3105..3124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3358..3375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3534..3561)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3653..3739)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3788..3810
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3802..3819
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3803..3819
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgaaggagaa gcagcgtctg
      121 agcaccagtc ggaaacggat agaatctcca ttcgcgaagt acaaccgttt ggggcagctg
      181 agttgtgccc tgtgtaacac tccggttaag agcgagctcc tgtggcagac tcacgtcctg
      241 ggaaagcagc accgagagaa agtggccgag ctgaaaggcg cgaaggaagc cagccagggt
      301 tcgtccgcca gttcagcgcc tcagtccgtc aagaggaaag cgccggacgc agacgaccaa
      361 gatgtcaaga gagcgaaggc caccttggtg cctcaggtac agccctccac atctgcgtgg
      421 accaccaact ttgacaaaat aggaaaggag ttcattagag cgactcccag taagccttca
      481 ggactcactt tactccccga ttatgaagat gaggaggagg aggaagagga ggaggaagga
      541 gatggagaaa gaaaaagggg ggacgccagc aagccgctct ccgacgcaca gggcaaggag
      601 cactcagttt cctcttcacg ggaggtaaca agtagtgtgc tgccaaacga tttctttagt
      661 actaatcctc ccaaggcccc cataattcct cattcagggt caattgagaa agcagaaata
      721 catgaaaaag tggtggaaag gagagaaaac accgcggaag cgttaccgga aggttttttt
      781 gacgaccctg aggtagatgc aagagtacga aaggttgatg ctccaaaaga tcagatggac
      841 aaagagtggg acgaattcca aaaagccatg aggcaggtca acactatttc cgaagccata
      901 gttgccgaag aggatgagga gggacggttg gaccgccaga ttggggagat cgatgagcag
      961 atagagtgtt accgacgggt ggaaaagcta cggaatcgcc aggatgaaat aaaaaataaa
     1021 cttaaagaaa tcctgaccat aaaagaactg cagaaaaagg aagaagagaa tgctgacagc
     1081 gatgatgagg gggaactaca ggatttgttg tctcaggatt ggagggtgaa aggggcattg
     1141 ttatacccaa ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1201 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1261 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1321 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc
     1381 agccaggaca gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg
     1441 tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa
     1501 tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     1561 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg
     1621 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     1681 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa
     1741 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     1801 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     1861 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     1921 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     1981 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     2041 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     2101 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     2161 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     2221 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     2281 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     2341 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     2401 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     2461 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     2521 taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     2941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3481 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3541 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3601 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3661 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3721 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3781 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
