LOCUS       sequence-364696-        9761 bp    DNA     circular SYN 14-APR-2025
DEFINITION  AAVS1 donor vector for constitutive gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9761)
  AUTHORS   Qian K, Huang CL, Chen H, Blackbourn LW 4th, Chen Y, Cao J, Yao L, 
            Sauvey C, Du Z, Zhang SC
  TITLE     A simple and efficient system for regulating gene expression in 
            human pluripotent stem cells and derivatives.
  JOURNAL   Stem Cells. 2014 Feb 4. doi: 10.1002/stem.1653.
  PUBMED    24497442
REFERENCE   2  (bases 1 to 9761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2014 Feb 4. doi: 10.1002/stem.1653."
COMMENT     Sequence Label: AAVS1-CAG-hrGFP
FEATURES             Location/Qualifiers
     source          1..9761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          108..1124
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1048..1070
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1132..1151
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1189..1908
                     /codon_start=1
                     /product="humanized Renilla green fluorescent protein"
                     /label=hrGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
                     QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
                     DGGLVEIRSDINLIEGMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
                     QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
                     AQLTSLGKPLGSLHEWV"
     primer_bind     complement(2010..2029)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2075..2130
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2129..2148)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2491..2507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2515..2531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2539..2545,2546..2563,2564..2569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2584..2605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    2679..3515
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(3549..3568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3550..3568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3575..3592)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3575..3591)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3584..3606)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             3729..4031
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3910..3929
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(4434..4453)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5048..5067
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(5428..6219,6220..6288))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6051..6070
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6412..7000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6901..6920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7154..7171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7288..7309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7324..7329,7330..7347,7348..7354)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7362..7378
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7386..7402
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7423..7441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    7480..8283
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    8290..8315
                     /label=SA
                     /note="splice acceptor site"
     CDS             8339..8392
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8402..9001
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8402..8421)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8898..8918
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(9033..9050)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9039..9263
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        9269..9648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
       61 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
      121 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
      181 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
      241 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
      301 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
      361 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
      421 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
      481 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
      541 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
      601 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
      661 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
      721 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
      781 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
      841 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
      901 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
      961 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     1021 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     1081 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1141 tggttattgt gctgtctcat cattttggca aagaattcgt cgaccaccat ggtgagcaag
     1201 cagatcctga agaacaccgg cctgcaggag atcatgagct tcaaggtgaa cctggagggc
     1261 gtggtgaaca accacgtgtt caccatggag ggctgcggca agggcaacat cctgttcggc
     1321 aaccagctgg tgcagatccg cgtgaccaag ggcgcccccc tgcccttcgc cttcgacatc
     1381 ctgagccccg ccttccagta cggcaaccgc accttcacca agtaccccga ggacatcagc
     1441 gacttcttca tccagagctt ccccgccggc ttcgtgtacg agcgcaccct gcgctacgag
     1501 gacggcggcc tggtggagat ccgcagcgac atcaacctga tcgaggggat gttcgtgtac
     1561 cgcgtggagt acaagggccg caacttcccc aacgacggcc ccgtgatgaa gaagaccatc
     1621 accggcctgc agcccagctt cgaggtggtg tacatgaacg acggcgtgct ggtgggccag
     1681 gtgatcctgg tgtaccgcct gaacagcggc aagttctaca gctgccacat gcgcaccctg
     1741 atgaagagca agggcgtggt gaaggacttc cccgagtacc acttcatcca gcaccgcctg
     1801 gagaagacct acgtggagga cggcggcttc gtagagcagc acgagaccgc catcgcccag
     1861 ctgaccagcc tgggcaagcc cctgggcagc ctgcacgagt gggtgtaaat cgatgatatc
     1921 acgcgtgaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     1981 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     2041 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     2101 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     2161 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     2221 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     2281 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     2341 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2401 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     2461 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     2521 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     2581 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2641 aaacctgtcg tgccagcgga tcgacagtac taagctttac tagggacagg attggtgaca
     2701 gaaaagcccc atccttaggc ctcctccttc ctagtctcct gatattgggt ctaaccccca
     2761 cctcctgtta ggcagattcc ttatctggtg acacaccccc atttcctgga gccatctctc
     2821 tccttgccag aacctctaag gtttgcttac gatggagcca gagaggatcc tgggagggag
     2881 agcttggcag ggggtgggag ggaagggggg gatgcgtgac ctgcccggtt ctcagtggcc
     2941 accctgcgct accctctccc agaacctgag ctgctctgac gcggctgtct ggtgcgtttc
     3001 actgatcctg gtgctgcagc ttccttacac ttcccaagag gagaagcagt ttggaaaaac
     3061 aaaatcagaa taagttggtc ctgagttcta actttggctc ttcacctttc tagtccccaa
     3121 tttatattgt tcctccgtgc gtcagtttta cctgtgagat aaggccagta gccagccccg
     3181 tcctggcagg gctgtggtga ggaggggggt gtccgtgtgg aaaactccct ttgtgagaat
     3241 ggtgcgtcct aggtgttcac caggtcgtgg ccgcctctac tccctttctc tttctccatc
     3301 cttctttcct taaagagtcc ccagtgctat ctgggacata ttcctccgcc cagagcaggg
     3361 tcccgcttcc ctaaggccct gctctgggct tctgggtttg agtccttggc aagcccagga
     3421 gaggcgctca ggcttccctg tcccccttcc tcgtccacca tctcatgccc ctggctctcc
     3481 tgccccttcc ctacaggggt tcctggctct gctctaaggg cgaattcgcg gccgctaaat
     3541 tcaattcgcc ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg
     3601 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     3661 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctat
     3721 acgtacggca gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg
     3781 atgtacagag tgatattatt gacacgccgg ggcgacggat ggtgatcccc ctggccagtg
     3841 cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg
     3901 aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag
     3961 aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct
     4021 ggggaatata aatgtcaggc atgagattat caaaaaggat cttcacctag atccttttca
     4081 cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta
     4141 tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat
     4201 ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg
     4261 cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa
     4321 ggatctgatg gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca
     4381 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4441 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4501 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4561 aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     4621 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     4681 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     4741 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     4801 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     4861 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg
     4921 gcgaggatct cgtcgtgacc catgcatggc gatgcctgct tgccgaatat catggtggaa
     4981 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     5041 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     5101 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     5161 cttgacgagt tcttctgaat tattaacgct tacaatttcc tgatgcggta ttttctcctt
     5221 acgcatctgt gcggtatttc acaccgcatc aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagattatc
     5341 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5401 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5461 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5521 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5581 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5641 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5701 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5761 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5821 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5881 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5941 tgtcatgcca tccgtaagat gcttttctgt gactggtgaa tactcaacca agtcattctg
     6001 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6061 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6121 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6181 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6241 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6301 tcaatattat tgaagcattt atcagggtta ttgtctcatg accaaaatcc cttaacgtga
     6361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6541 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7021 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7081 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7141 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7201 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7261 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7321 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7381 tcacacagga aacagctatg accatgatta cgccaagctc agaattaacc ctcactaaag
     7441 ggactagcta gtcctgcagg tttaaacgaa ttcgcccttt gctttctctg accagcattc
     7501 tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg gcctgggtca cctctacggc
     7561 tggcccagat ccttccctgc cgcctccttc aggttccgtc ttcctccact ccctcttccc
     7621 cttgctctct gctgtgttgc tgcccaagga tgctctttcc ggagcacttc cttctcggcg
     7681 ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc
     7741 tctcctccct cacccaaccc catgccgtct tcactcgctg ggttcccttt tccttctcct
     7801 tctggggcct gtgccatctc tcgtttctta ggatggcctt ctccgacgga tgtctccctt
     7861 gcgtcccgcc tccccttctt gtaggcctgc atcatcaccg tttttctgga caaccccaaa
     7921 gtaccccgtc tccctggctt tagccacctc tccatcctct tgctttcttt gcctggacac
     7981 cccgttctcc tgtggattcg ggtcacctct cactcctttc atttgggcag ctcccctacc
     8041 ccccttacct ctctagtctg tgctagctct tccagccccc tgtcatggca tcttccaggg
     8101 gtccgagagc tcagctagtc ttcttcctcc aacccgggcc cctatgtcca cttcaggaca
     8161 gcatgtttgc tgcctccagg gatcctgtgt ccccgagctg ggaccacctt atattcccag
     8221 ggccggttaa tgtggctctg gttctgggta cttttatctg tcccctccac cccacagtgg
     8281 ggcaagcttc tgacctcttc tcttcctccc acagggcctc gagagatctg gcagcggaga
     8341 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctaggctcga
     8401 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     8461 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8521 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8581 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     8641 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8701 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8761 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8821 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     8881 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     8941 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     9001 atctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     9061 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     9121 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     9181 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     9241 tggggatgcg gtgggctcta tgggtctcga cattgattat tgactagtta ttaatagtaa
     9301 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     9361 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     9421 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     9481 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9541 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9601 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     9661 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     9721 tttatttttt aattattttg tgcagcgatg ggggcggggg g
//
