LOCUS       sequence-365344-        1781 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent 
            protein miRFP670nano3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Oliinyk OS, Baloban M, Clark CL, Carey E, Pletnev S, Nimmerjahn A, 
            Verkhusha VV
  TITLE     Single-domain near-infrared protein provides a scaffold for 
            antigen-dependent fluorescent nanobodies.
  JOURNAL   Nat Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 
            10.1038/s41592-022-01467-6.
  PUBMED    35606446
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2022 May 23. pii: 10.1038/s41592-022-01467-6. doi: 
            10.1038/s41592-022-01467-6."
COMMENT     Sequence Label: pLAMP1-miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(24..86,87..1244)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
ORIGIN
        1 ggactcagat ctcgagcgcc accatggcgg ccccgggcgc ccggcggccg ctgctcctgt
       61 tgctgctggc aggccttgca cacagcgccc cagcactgtt cgaggtgaaa gacaacaacg
      121 gcacagcgtg tataatggcc agcttctctg cctcctttct gaccacctat gaggctggac
      181 atgtttctaa ggtctcgaat atgaccctgc cagcctctgc agaagtcctg aagaatagca
      241 gctcttgtgg tgaaaagaat gcttctgagc ccaccctcgc aatcaccttt ggagaaggat
      301 atttactgaa actcaccttc acaaaaaaca caacacgtta cagtgtccag cacatgtatt
      361 tcacatataa cctgtcagac acacaattct ttcccaatgc cagctccaaa gggcccgaca
      421 ctgtggattc cacaactgac atcaaggcag acatcaacaa aacataccga tgtgtcagcg
      481 acatcagggt ctacatgaag aatgtgacca ttgtgctctg ggacgctact atccaggcct
      541 acctgccgag tagcaacttc agcaaggaag agacacgctg cccacaggat caaccttccc
      601 caactactgg gccacccagc ccctcaccac cacttgtgcc cacaaacccc agtgtgtcca
      661 agtacaatgt gactggtgac aatggaacct gcctgctggc ctctatggca ctgcaactca
      721 acatcaccta catgaagaag gacaacacga ctgtgaccag agcattcaac atcaacccaa
      781 gtgacaaata tagtgggact tgcggtgccc agttggtgac cctgaaggtg gggaacaaga
      841 gcagagtcct ggagctgcag tttgggatga atgccacttc tagcctgttt ttcctgcaag
      901 gagttcagtt gaacatgact cttcctgatg ccatagagcc cacgttcagc acctccaact
      961 attccctgaa agctcttcag gccagtgtcg gcaactcata caagtgcaac agtgaggagc
     1021 acatctttgt cagcaaggcg ctcgccctca atgtcttcag cgtgcaagtc caggctttca
     1081 gggtagaaag tgacaggttt gggtctgtgg aagagtgtgt acaggacggt aacaacatgc
     1141 tgatccccat tgctgtgggc ggggccctgg cagggctggt cctcatcgtc ctcatcgcct
     1201 acctcatcgg caggaagagg agtcacgcgg gctatcagac catctcggaa ttcggctcca
     1261 ccggctccac cggctccacc ggcgcggatc caccggtaat ggcaaacctg gacaagatgc
     1321 tgaacaccac cgtgaccgag gtgcgcaagt tcctgcaagc ggacagagtg tgcgtgttca
     1381 agttcgagga agattactcc ggcaccgtct cgcacgaagc cgtggacgac agatggatta
     1441 gcatcctgaa gacccaggtg caggacagat acttcatgga aaccagaggc gaggaatacg
     1501 tccacggcag ataccaggcc atcgccgaca tctacacagc caatctggtc gagtgctaca
     1561 gagacctgct gatcgagttt caggtgcggg ccattctggc tgtccccatc ctgcaaggca
     1621 agaagctgtg gggcctgctg gtggcccacc aactggccgg ccctcgggag tggcagacct
     1681 gggaaatcga cttcctgaaa cagcaagccg tggtgatggg catcgccatc cagcagagct
     1741 agctgaattc tgcagatatc cagcacagtg gcggccgcga c
//
