LOCUS       sequence-365564-        1128 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of LuxA via a T7 promoter in TXTL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Sato W, Rasmussen M, Deich C, Engelhart AE, Adamala KP
  TITLE     Expanding luciferase reporter systems for cell-free protein 
            expression.
  JOURNAL   Sci Rep. 2022 Jul 7;12(1):11489. doi: 10.1038/s41598-022-15624-6.
  PUBMED    35798760
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-07-7"; volume: "12(1):11489. doi"; pages: " 
            10.1038/s41598-022-15624-6"
COMMENT     Sequence Label: pTXTL-T7max-LuxA
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctcnnntnta nnntagcgac gttgtttttc tttaagaaat ggcatgacat cagactggaa
       61 gagcttcatg gaagcaataa tttcgtctac tgttccatta gcttcaaatc cacaacaaat
      121 atttgatatt cctgtagcat caatgtcttt ttgaattatg tcaatacatt cctgcggcgt
      181 tcccacggga ttgatttcgt aactgtaatc aatacggcga ttagtatctt tatgtccttt
      241 taatacaaag tcacgccact gccctttatt gaaatcataa cctcttgttt ggtctgaatc
      301 atcaaaaata gtcgtagcat tcacataaga atcataccaa tgccccagaa atttccggca
      361 aatctctttc gctttaattg agtcatgatc tacagatgtt atatatgata agcaatggtc
      421 gatattatga atatcgtgcc catattcttg agccacttca ttataaagct caagttgtgc
      481 tttcttttcg ttagtattta taatccaact taatatcatc ggtaggccaa attgagcagc
      541 ccactcagtc gtcgaagctg attcagccac cacataaacc ggtgcgccac ctctgctata
      601 cgccgcgggg tttactttta ccttatggaa cttgatatgt tcattatcag cttccatata
      661 tccctctgtc atgccattct ttatcagccc gtaccagcat tccgctaagg cgcgactgtt
      721 attcatatct gtgccgaata cgcgaaagtc cttgttgtaa agccctcggc aaataccaaa
      781 ccgaaatcgt ccttttgaca tttgatccaa taaattcaca tcttcaagtt ggcgtactgg
      841 atgggctgtg ggaagaacaa tagcggcagt tcctacattc aattttttta gtcgcgccaa
      901 gtaaatatgc agcagcgaca taannggttt accaagcaaa ccnaactncc gtgaaatgat
      961 gctnccagta anccatannn tatcaaaacc acactccnca ganatngcga cnnatttnan
     1021 cnaaacgttt cattnnnntn nntganaaan ntngnggnnn nnnnnntngn nagcnaaaaa
     1081 nnnnnnaann nnntnnnant cntancnnnn tatcnnnnnt nnntnaaa
//
