LOCUS       sequence-365768-        4830 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC26A6_STOP
FEATURES             Location/Qualifiers
     source          1..4830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2387..2486)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2503..2522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2504..2522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2527..2543)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2527..2543)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2645..2664)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2656..3465
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2723..2742)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3612..4200
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4101..4120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4354..4371
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4530..4557)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4649..4735)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4784..4806
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4798..4815
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4799..4815
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggg actggcagac
      121 gcaagcggac caagggatac acaggccctg ctgtccgcca cccaggcaat ggacctgcgg
      181 agaagggatt accacatgga gcgcccactg ctgaatcagg agcacctgga ggagctgggc
      241 cggtggggat ccgccccacg cacacaccag tggcggacct ggctgcagtg ctctagggca
      301 agggcatacg cactgctgct gcagcacctg cccgtgctgg tgtggctgcc aagatatcct
      361 gtgagggact ggctgctggg cgatctgctg tccggcctgt ctgtggccat catgcagctg
      421 cctcagggcc tggcatacgc actgctggca ggactgccac ccgtgttcgg cctgtacagc
      481 tccttctatc cagtgtttat ctatttcctg tttggcacat cccgccacat ctctgtgggc
      541 acctttgccg tgatgtccgt gatggtgggc agcgtgaccg agtccctggc accacaggcc
      601 ctgaacgact ctatgatcaa tgagacagca agggatgcag caagggtgca ggtggcaagc
      661 accctgtccg tgctggtggg actgttccaa gtgggactgg gcctgatcca cttcggcttt
      721 gtggtgacat acctgtccga gcctctggtg cggggatata ccacagcagc agcagtgcag
      781 gtgttcgtga gccagctgaa gtacgtgttt ggcctgcacc tgtctagcca ctctggcccc
      841 ctgagcctga tctataccgt gctggaggtg tgctggaagc tgcctcagtc caaagtggga
      901 acagtggtga ccgcagcagt ggcaggagtg gtgctggtgg tggtgaagct gctgaatgat
      961 aagctgcagc agcagctgcc tatgccaatc ccaggagagc tgctgacact gatcggagca
     1021 accggaatca gctacggaat gggcctgaag cacaggttcg aggtggacgt ggtgggaaac
     1081 atcccagcag gactggtgcc accagtggca cccaatacac agctgttttc taagctggtg
     1141 ggaagcgcct tcaccatcgc agtggtgggc tttgccatcg ccatcagcct gggcaagatc
     1201 ttcgccctga gacacggcta tagggtggac tctaaccagg agctggtggc cctgggcctg
     1261 agcaatctga tcggcggcat cttccagtgc tttccagtgt cctgttctat gagcagatcc
     1321 ctggtgcagg agtccaccgg aggaaactct caggtggcag gagccatctc ctctctgttc
     1381 atcctgctga tcatcgtgaa gctgggcgag ctgtttcacg atctgccaaa ggccgtgctg
     1441 gcagcaatca tcatcgtgaa cctgaagggc atgctgagac agctgtccga catgaggtct
     1501 ctgtggaagg ccaatagggc cgatctgctg atctggctgg tgaccttcac agccaccatc
     1561 ctgctgaacc tggacctggg cctggtggtg gccgtgatct ttagcctgct gctggtggtg
     1621 gtgagaacac agatgcctca ctactccgtg ctgggccagg tgccagacac cgatatctac
     1681 agggatgtgg ccgagtattc cgaggcaaag gaggtgcgcg gagtgaaggt gttccggagc
     1741 tccgccacag tgtacttcgc caacgccgag ttttattctg acgccctgaa gcagagatgc
     1801 ggcgtggacg tggattttct gatcagccag aagaagaagc tgctgaagaa gcaggagcag
     1861 ctgaagctga agcagctgca gaaggaggag aagctgagga agcaggcagc aagccctaag
     1921 ggagcatctg tgagcatcaa cgtgaatacc agcctggagg acatgcggtc caacaatgtg
     1981 gaggattgta agatgatgca ggtgtctagc ggcgacaaga tggaggatgc cacagccaat
     2041 ggccaggagg actctaaggc cccagatgga agcaccctga aggccctggg actgcctcag
     2101 ccagacttcc actccctgat cctggatctg ggcgccctgt cttttgtgga cacagtgtgc
     2161 ctgaagagcc tgaagaacat cttccacgac ttccgggaga tcgaggtgga ggtgtatatg
     2221 gcagcatgtc acagcccagt ggtgtcccag ctggaggcag gacacttctt tgacgcctcc
     2281 atcacaaaga agcacctgtt cgcctctgtg cacgatgccg tgacctttgc cctgcagcac
     2341 cccagacctg tgccagactc tcctgtgagc gtgaccaggc tgtaagaccc agctttcttg
     2401 tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact
     2461 atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat
     2521 tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac
     2581 attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt
     2641 aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc
     2701 aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt
     2761 gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc
     2821 aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa
     2881 tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc
     2941 accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt
     3001 gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt
     3061 aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat
     3121 aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa
     3181 gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt
     3241 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt
     3301 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt
     3361 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat
     3421 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat
     3481 tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca
     3541 aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc
     3601 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3661 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3721 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3781 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3841 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3901 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3961 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4021 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     4081 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     4141 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4201 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4261 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4321 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4381 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     4441 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc
     4501 gctagccagg aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     4561 tagtttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     4621 tcacaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     4681 aacaacagat aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc
     4741 tggcagttcc ctactctcgc gttaacgcta gcatggatgt tttcccagtc acgacgttgt
     4801 aaaacgacgg ccagtcttaa gctcgggccc
//
