LOCUS       sequence-366030-        8891 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Doxycycline-dependent expression of human cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTRE3G-human cGAS-GFP
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        355..558
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             559..734
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    797..996
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1197..1613
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1543..1565
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1581..1597
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1653..2002
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1661..1679
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1697..1715
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1733..1751
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1769..1787
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1805..1823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1841..1859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1877..1895
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1941..1965
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3596..3598,3599..4312)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3596..3598,3599..4309)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3638..3659)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3899..3918)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4246..4267
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        4343..4842
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4378..4401)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4775..4794
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4863..5462
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4863..4882)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5359..5379
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5643..6068
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6171..6193)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6329..6349)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6334..6663
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6514..6649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6685..6704
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6785..6802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6956..7544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7036..7055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7715..8506,8507..8575))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8338..8357
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8576..8680)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8748..8766
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8866..8891,1..354)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
       61 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      121 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      181 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      241 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      301 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      361 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      421 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      481 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      541 gtctatataa gcagagctca ataaaagagc ccacaacccc tcactcggcg cgccagtctt
      601 ccgatagact gcgtcgcccg ggtacccgta ttcccaataa agcctcttgc tgtttgcatc
      661 cgaatcgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccacgac
      721 gggggtcttt catttggggg ctcgtccggg atttggagac ccctgcccag ggaccaccga
      781 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt
      841 ctatgtttga tgttatgcgc ctgcgtctgt actagttagc taactagctc tgtatctggc
      901 ggacccgtgg tggaactgac gagttctgaa cacccggccg caaccctggg agacgtccca
      961 gggactttgg gggccgtttt tgtggcccga cctgaggaag ggagtcgatg tggaatccga
     1021 ccccgtcagg atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt
     1081 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
     1141 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
     1201 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1261 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1321 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1381 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1441 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1501 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1561 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggaattg
     1621 aagatctcga tgaggccctt tcgtcttcac tcgagtttac tccctatcag tgatagagaa
     1681 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     1741 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     1801 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     1861 cgtatatcca gtttactccc tatcagtgat agagaacgta tgtcgaggta ggcgtgtacg
     1921 gtgggcgcct ataaaagcag agctcgttta gtgaaccgtc agatcgcctg gagcaattcc
     1981 acaacacttt tgtcttatac ttggatccgc caccatgcag ccttggcacg gaaaggccat
     2041 gcagagagct tccgaggccg gagccactgc ccccaaggct tccgcacgga atgccagggg
     2101 cgccccgatg gatcccaccg agtctccggc tgcccccgag gccgccctgc ctaaggcggg
     2161 aaagttcggc cccgccagga agtcgggatc ccggcagaaa aagagcgccc cggacaccca
     2221 ggagaggccg cccgtccgcg caactggggc ccgcgccaaa aaggcccctc agcgcgccca
     2281 ggacacgcag ccgtctgacg ccaccagcgc ccctggggca gaggggctgg agcctcctgc
     2341 ggctcgggag ccggctcttt ccagggctgg ttcttgccgc cagaggggcg cgcgctgctc
     2401 cacgaagcca agacctccgc ccgggccctg ggacgtgccc agccccggcc tgccggtctc
     2461 ggcccccatt ctcgtacgga gggatgcggc gcctggggcc tcgaagctcc gggcggtttt
     2521 ggagaagttg aagctcagcc gcgatgatat ctccacggcg gcggggatgg tgaaaggggt
     2581 tgtggaccac ctgctgctca gactgaagtg cgactccgcg ttcagaggcg tcgggctgct
     2641 gaacaccggg agctactatg agcacgtgaa gatttctgca cctaatgaat ttgatgtcat
     2701 gtttaaactg gaagtcccca gaattcaact agaagaatat tccaacactc gtgcatatta
     2761 ctttgtgaaa tttaaaagaa atccgaaaga aaatcctctg agtcagtttt tagaaggtga
     2821 aatattatca gcttctaaga tgctgtcaaa gtttaggaaa atcattaagg aagaaattaa
     2881 cgacattaaa gatacagatg tcatcatgaa gaggaaaaga ggagggagcc ctgctgtaac
     2941 acttcttatt agtgaaaaaa tatctgtgga tataaccctg gctttggaat caaaaagtag
     3001 ctggcctgct agcacccaag aaggcctgcg cattcaaaac tggctttcag caaaagttag
     3061 gaagcaacta cgactaaagc cattttacct tgtacccaag catgcaaagg aaggaaatgg
     3121 tttccaagaa gaaacatggc ggctatcctt ctctcacatc gaaaaggaaa ttttgaacaa
     3181 tcatggaaaa tctaaaacgt gctgtgaaaa caaagaagag aaatgttgca ggaaagattg
     3241 tttaaaacta atgaaatacc ttttagaaca gctgaaagaa aggtttaaag acaaaaaaca
     3301 tctggataaa ttctcttctt atcatgtgaa aactgccttc tttcacgtat gtacccagaa
     3361 ccctcaagac agtcagtggg accgcaaaga cctgggcctc tgctttgata actgcgtgac
     3421 atactttctt cagtgcctca ggacagaaaa acttgagaat tattttattc ctgaattcaa
     3481 tctattctct agcaacttaa ttgacaaaag aagtaaagaa tttctgacaa agcaaattga
     3541 atatgaaaga aacaatgagt ttccagtttt tgatgaattt ggtggcggag gtagtgtgag
     3601 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     3661 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3721 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3781 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3841 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3901 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3961 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     4021 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     4081 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     4141 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     4201 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     4261 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc
     4321 gccggcgggc ccgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     4381 tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca
     4441 cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca
     4501 ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg
     4561 acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag
     4621 caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc
     4681 tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc
     4741 ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg
     4801 tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta
     4861 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     4921 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     4981 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5041 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5101 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5161 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5221 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5281 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5341 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5401 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5461 gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg
     5521 cgtctcgaga tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag
     5581 cctatagagt acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg
     5641 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag
     5701 gcatggaaaa atacataact gagaatagag aagttcagat caaggtcagg aacagatgga
     5761 acagggtcga ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga
     5821 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct
     5881 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt
     5941 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg
     6001 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt
     6061 ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc
     6121 accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     6181 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     6241 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     6301 agcgatagcg gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     6361 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     6421 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6481 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     6661 aaaaagctta ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     6721 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     6781 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6841 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6901 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6961 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7021 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7081 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7141 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7201 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7261 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7321 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7381 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7441 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     7501 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7561 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7621 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7681 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7741 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     7801 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7861 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7921 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7981 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     8041 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     8101 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     8161 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     8221 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     8281 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     8341 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     8401 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     8461 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     8521 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8581 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8641 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     8701 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     8761 cgaggccctt tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata
     8821 atatgtacat ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga
     8881 ctagttatta a
//
