LOCUS       sequence-366048-        9197 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pseudovirus plasmid that expresses spike of BA.2.12.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Dacon C, Tucker C, Peng L, Lee CD, Lin TH, Yuan M, Cong Y, Wang L, 
            Purser L, Williams JK, Pyo CW, Kosik I, Hu Z, Zhao M, Mohan D, 
            Cooper AJR, Peterson M, Skinner J, Dixit S, Kollins E, Huzella L, 
            Perry D, Byrum R, Lembirik S, Drawbaugh D, Eaton B, Zhang Y, Yang 
            ES, Chen M, Leung K, Weinberg RS, Pegu A, Geraghty DE, Davidson E, 
            Douagi I, Moir S, Yewdell JW, Schmaljohn C, Crompton PD, Holbrook 
            MR, Nemazee D, Mascola JR, Wilson IA, Tan J
  TITLE     Broadly neutralizing antibodies target the coronavirus fusion 
            peptide.
  JOURNAL   Science. 2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773.
  PUBMED    35857439
REFERENCE   2  (bases 1 to 9197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Jul 12:eabq3773. doi: 10.1126/science.abq3773."
COMMENT     Sequence Label: pcDNA3.3_SARS2_BA.2.12.1
FEATURES             Location/Qualifiers
     source          1..9197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4687..4706)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4731..4779
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4981..5409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5068..5087)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5278..5299
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5418..5438)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5423..5752
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5603..5738
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5665..5684
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5819..6613
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5873..5892)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6483..6502
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6789..6910
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6826..6845)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6880..6899
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6959..6975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6959..6975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6972..6994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6983..6999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7007..7013,7014..7031,7032..7037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7052..7073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7190..7207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7361..7949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7441..7460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8120..8911,8912..8980))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8743..8762
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8981..9085)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9160..9179)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
       61 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
      121 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      781 gttttgacct ccatagaaga caccgggacc gatccagcct ccggactgag ctccttaagg
      841 ttaactctag agaattcgga tccatgtttg tgttcctggt gctgctgcca ctggtgtcca
      901 gccagtgtgt gaacctgatc accaggaccc aatcctacac caactccttc accaggggag
      961 tctactaccc tgacaaggtg ttcaggtcct ctgtgctgca cagcacccag gacctgttcc
     1021 tgccattctt cagcaatgtg acctggttcc atgccatcca tgtgtctggc accaatggca
     1081 ccaagaggtt tgacaaccct gtgctgccat tcaatgatgg agtctacttt gccagcacag
     1141 agaagagcaa catcatcagg ggctggattt ttggcaccac cctggacagc aagacccagt
     1201 ccctgctgat tgtgaacaat gccaccaatg tggtgattaa ggtgtgtgag ttccagttct
     1261 gtaatgaccc attcctggac gtctactacc acaagaacaa caagtcctgg atggagtctg
     1321 agttcagggt ctactcctct gccaacaact gtacctttga atatgtgagc caaccattcc
     1381 tgatggactt ggagggcaag cagggcaact tcaagaacct gagggagttt gtgttcaaga
     1441 acattgatgg ctacttcaag atttacagca aacacacacc aatcaacctg gggagggacc
     1501 tgccacaggg cttctctgcc ttggaaccac tggtggacct gccaattggc atcaacatca
     1561 ccaggttcca gaccctgctg gctctgcaca ggtcctacct gacacctgga gactcctcct
     1621 ctggctggac agcaggagca gcagcctact atgtgggcta cctccaacca aggaccttcc
     1681 tgctgaaata caatgagaat ggcaccatca cagatgctgt ggactgtgcc ctggacccac
     1741 tgtctgagac caagtgtacc ctgaaatcct tcacagtgga gaagggcatc taccagacca
     1801 gcaacttcag ggtccaacca acagagagca ttgtgaggtt tccaaacatc accaacctgt
     1861 gtccatttga cgaggtgttc aatgccacca ggtttgcctc tgtctatgcc tggaacagga
     1921 agaggattag caactgtgtg gctgactact ctgtgctcta caactttgcc cccttcttcg
     1981 ccttcaagtg ttatggagtg agcccaacca aactgaatga cctgtgtttc accaatgtct
     2041 atgctgactc ctttgtgatt aggggaaatg aggtgagtca gattgcccct ggacaaacag
     2101 gcaacattgc tgactacaac tacaaactgc ctgatgactt cacaggctgt gtgattgcct
     2161 ggaacagcaa caagctggac agcaaggtgg gaggcaacta caactaccag tacagactgt
     2221 tcaggaagag caacctgaaa ccatttgaga gggacatcag cacagagatt taccaggctg
     2281 gcaacaaacc atgtaatgga gtggcgggct tcaactgtta ctttccactc agatcctatg
     2341 gcttcagacc aacctatgga gtgggccacc aaccatacag ggtggtggtg ctgtcctttg
     2401 aactgctcca tgcccctgcc acagtgtgtg gaccaaagaa gagcaccaac ctggtgaaga
     2461 acaagtgtgt gaacttcaac ttcaatggac tgacaggcac aggagtgctg acagagagca
     2521 acaagaagtt cctgccattc caacagtttg gcagggacat tgctgacacc acagatgctg
     2581 tgagggaccc acagaccttg gagattctgg acatcacacc atgttccttt ggaggagtgt
     2641 ctgtgattac acctggcacc aacaccagca accaggtggc tgtgctctac cagggtgtga
     2701 actgtactga ggtgcctgtg gctatccatg ctgaccaact tacaccaacc tggagggtct
     2761 acagcacagg cagcaatgtg ttccagacca gggctggctg tctgattgga gcagagtatg
     2821 tgaacaactc ctatgagtgt gacatcccaa ttggagcagg catctgtgcc tcctaccaga
     2881 cccagaccaa gagccatagg agggcaaggt ctgtggcaag ccagagcatc attgcctaca
     2941 caatgagtct gggagcagag aaccttgtgg cttacagcaa caacagcatt gccatcccaa
     3001 ccaacttcac catctctgtg accacagaaa ttctgcctgt gagtatgacc aagacctctg
     3061 tggactgtac aatgtatatc tgtggagaca gcacagagtg tagcaacctg ctgctccaat
     3121 atggctcctt ctgtacccaa cttaagaggg ctctgacagg cattgctgtg gaacaggaca
     3181 agaacaccca ggaggtgttt gcccaggtga agcagattta caagacacct ccaatcaagt
     3241 actttggagg cttcaacttc agccagattc tgcctgaccc aagcaagcca agcaagaggt
     3301 ccttcattga ggacctgctg ttcaacaagg tgaccctggc tgatgctggc ttcatcaagc
     3361 aatatggaga ctgtctggga gacattgctg caagggacct gatttgtgcc cagaagttca
     3421 atggactgac cgtgctgcct ccactgctga cagatgagat gattgcccaa tacacctctg
     3481 ccctgctggc tggcaccatc acctctggct ggacctttgg agcaggagca gccctccaaa
     3541 tcccatttgc tatgcagatg gcttacaggt tcaatggcat tggagtgacc cagaatgtgc
     3601 tctatgagaa ccagaaactg attgccaacc agttcaactc tgccattggc aagattcagg
     3661 actccctgtc cagcacagcc tctgccctgg gcaaactcca agatgtggtg aaccataatg
     3721 cccaggctct gaacaccctg gtgaagcaac tttccagcaa gtttggagcc atctcctctg
     3781 tgctgaatga catcctgagc agactggaca aggtggaggc tgaggtccag attgacagac
     3841 tgattacagg cagactccaa tccctccaaa cctatgtgac ccaacaactt atcagggctg
     3901 ctgagattag ggcatctgcc aacctggctg ccaccaagat gagtgagtgt gtgctgggac
     3961 aaagcaagag ggtggacttc tgtggcaagg gctaccacct gatgagtttt ccacagtctg
     4021 cccctcatgg agtggtgttc ctgcatgtga cctatgtgcc tgcccaggag aagaacttca
     4081 ccacagcccc tgccatctgc catgatggca aggctcactt tccaagggag ggagtgtttg
     4141 tgagcaatgg cacccactgg tttgtgaccc agaggaactt ctatgaacca cagattatca
     4201 ccacagacaa cacctttgtg tctggcaact gtgatgtggt gattggcatt gtgaacaaca
     4261 cagtctatga cccactccaa cctgaactgg actccttcaa ggaggaactg gacaaatact
     4321 tcaagaacca caccagccct gatgtggacc tgggagacat ctctggcatc aatgcctctg
     4381 tggtgaacat ccagaaggag attgacagac tgaatgaggt ggctaagaac ctgaatgagt
     4441 ccctgattga cctccaagaa ctgggcaaat atgaacaata catcaagtgg ccatggtaca
     4501 tctggctggg cttcattgct ggactgattg ccattgtgat ggtgaccata atgctgtgtt
     4561 gtatgacctc ctgttgttcc tgtctgaaag gctgttgttc ctgtggctcc tgttgtaagt
     4621 gactcgagcc atggaagctt gatatctaac tgactgaacc ggtggtacca aacgggggag
     4681 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     4741 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     4801 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     4861 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     4921 tcggggcggc aggccctgcc atagcagatc tgcgcagctg gggctctagg gggtatcccc
     4981 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5041 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5101 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5161 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5221 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5281 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5341 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     5401 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5461 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5521 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5581 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5641 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     5701 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     5761 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     5821 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5881 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5941 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     6001 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     6061 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     6121 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     6181 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     6241 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     6301 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     6361 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     6421 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     6481 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     6541 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     6601 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc
     6661 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     6721 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     6781 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6841 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6901 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6961 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7021 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7081 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7141 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7201 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7261 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7321 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7381 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7441 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7501 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7561 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7621 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7681 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7741 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7801 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7981 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8041 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8101 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8161 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8221 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8281 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8341 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8401 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8461 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8521 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8581 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8641 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8701 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8761 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8821 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8881 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8941 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9001 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9061 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9121 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
     9181 atagttaagc cagtatc
//
