LOCUS       sequence-366266-       12232 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Flag-LRRK2 K17A/K18A/R1441G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Vides EG, Adhikari A, Chiang CY, Lis P, Purlyte E, Limouse C, 
            Shumate JL, Spinola-Lasso E, Dhekne HS, Alessi DR, Pfeffer SR
  TITLE     A feed-forward pathway drives LRRK2 kinase membrane recruitment and 
            activation.
  JOURNAL   Elife. 2022 Sep 23;11:e79771. doi: 10.7554/eLife.79771.
  PUBMED    36149401
REFERENCE   2  (bases 1 to 12232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-23"; volume: "11:e79771. doi"; pages: " 
            10.7554/eLife.79771"
COMMENT     Sequence Label: pCMV Flag-LRRK2 K17A/K18A/R1441G
FEATURES             Location/Qualifiers
     source          1..12232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        200..403
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     354..374
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      427..436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             436..459
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    8075..8697
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8274..8293)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(8721..8741)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8726..9055
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8906..9041
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9093..9112)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9094..9112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9126..9142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9126..9142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9139..9161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9150..9166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9174..9180,9181..9198,9199..9204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9219..9240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9357..9374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9528..10116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9608..9627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10287..11078,11079..11147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10910..10929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11148..11252)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11279..11734
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11366..11385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11576..11597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11860..11882
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11874..11891
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11875..11891
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        join(12052..12232,1..199)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
       61 agtgtatcat atgccaagtc cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      121 gcattatgcc cagtacatga ccttacggga ctttcctact tggcagtaca tctacgtatt
      181 agtcatcgct attaccatgg tgatgcggtt ttggcagtac accaatgggc gtggatagcg
      241 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      301 gcaccaaaat caacgggact ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat
      361 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
      421 gaattggcca ccatggacta caaggacgat gacgataagg gatccatggc tagtggcagc
      481 tgtcaggggt gcgaagagga cgaggaaact ctggcagcat tgatagtcag gctgaacaat
      541 gtccaggaag gaaaacagat agaaacgctg gtccaaatcc tggaggatct gctggtgttc
      601 acgtactccg agcacgcctc caagttattt caaggcaaaa atatccatgt gcctctgttg
      661 atcgtcttgg actcctatat gagagtcgcg agtgtgcagc aggtgggttg gtcacttctg
      721 tgcaaattaa tagaagtctg tccaggtaca atgcaaagct taatgggacc ccaggatgtt
      781 ggaaatgatt gggaagtcct tggtgttcac caattgattc ttaaaatgct aacagttcat
      841 aatgccagtg taaacttgtc agtgattgga ctgaagacct tagatctcct cctaacttca
      901 ggtaaaatca ccttgctgat attggatgaa gaaagtgata ttttcatgtt aatttttgat
      961 gccatgcact catttccagc caatgatgaa gtccagaaac ttggatgcaa agctttacat
     1021 gtgctgtttg agagagtctc agaggagcaa ctgactgaat ttgttgagaa caaagattat
     1081 atgatattgt taagtgcgtt aacaaatttt aaagatgaag aggaaattgt gcttcatgtg
     1141 ctgcattgtt tacattccct agcgattcct tgcaataatg tggaagtcct catgagtggc
     1201 aatgtcaggt gttataatat tgtggtggaa gctatgaaag cattccctat gagtgaaaga
     1261 attcaagaag tgagttgctg tttgctccat aggcttacat taggtaattt tttcaatatc
     1321 ctggtattaa acgaagtcca tgagtttgtg gtgaaagctg tgcagcagta cccagagaat
     1381 gcagcattgc agatctcagc gctcagctgt ttggccctcc tcactgagac tattttctta
     1441 aatcaagatt tagaggaaaa gaatgagaat caagagaatg atgatgaggg ggaagaagat
     1501 aaattgtttt ggctggaagc ctgttacaaa gcattaacgt ggcatagaaa gaacaagcac
     1561 gtgcaggagg ccgcatgctg ggcactaaat aatctcctta tgtaccaaaa cagtttacat
     1621 gagaagattg gagatgaaga tggccatttc ccagctcata gggaagtgat gctctccatg
     1681 ctgatgcatt cttcatcaaa ggaagttttc caggcatctg cgaatgcatt gtcaactctc
     1741 ttagaacaaa atgttaattt cagaaaaata ctgttatcaa aaggaataca cctgaatgtt
     1801 ttggagttaa tgcagaagca tatacattct cctgaagtgg ctgaaagtgg ctgtaaaatg
     1861 ctaaatcatc tttttgaagg aagcaacact tccctggata taatggcagc agtggtcccc
     1921 aaaatactaa cagttatgaa acgtcatgag acatcattac cagtgcagct ggaggcgctt
     1981 cgagctattt tacattttat agtgcctggc atgccagaag aatccaggga ggatacagaa
     2041 tttcatcata agctaaatat ggttaaaaaa cagtgtttca agaatgatat tcacaaactg
     2101 gtcctagcag ctttgaacag gttcattgga aatcctggga ttcagaaatg tggattaaaa
     2161 gtaatttctt ctattgtaca ttttcctgat gcattagaga tgttatccct ggaaggtgct
     2221 atggattcag tgcttcacac actgcagatg tatccagatg accaagaaat tcagtgtctg
     2281 ggtttaagtc ttataggata cttgattaca aagaagaatg tgttcatagg aactggacat
     2341 ctgctggcaa aaattctggt ttccagctta taccgattta aggatgttgc tgaaatacag
     2401 actaaaggat ttcagacaat cttagcaatc ctcaaattgt cagcatcttt ttctaagctg
     2461 ctggtgcatc attcatttga cttagtaata ttccatcaaa tgtcttccaa tatcatggaa
     2521 caaaaggatc aacagtttct aaacctctgt tgcaagtgtt ttgcaaaagt agctatggat
     2581 gattacttaa aaaatgtgat gctagagaga gcgtgtgatc agaataacag catcatggtt
     2641 gaatgcttgc ttctattggg agcagatgcc aatcaagcaa aggagggatc ttctttaatt
     2701 tgtcaggtat gtgagaaaga gagcagtccc aaattggtgg aactcttact gaatagtgga
     2761 tctcgtgaac aagatgtacg aaaagcgttg acgataagca ttgggaaagg tgacagccag
     2821 atcatcagct tgctcttaag gaggctggcc ctggatgtgg ccaacaatag catttgcctt
     2881 ggaggatttt gtataggaaa agttgaacct tcttggcttg gtcctttatt tccagataag
     2941 acttctaatt taaggaaaca aacaaatata gcatctacac tagcaagaat ggtgatcaga
     3001 tatcagatga aaagtgctgt ggaagaagga acagcctcag gcagcgatgg aaatttttct
     3061 gaagatgtgc tgtctaaatt tgatgaatgg acctttattc ctgactcttc tatggacagt
     3121 gtgtttgctc aaagtgatga cctggatagt gaaggaagtg aaggctcatt tcttgtgaaa
     3181 aagaaatcta attcaattag tgtaggagaa ttttaccgag atgccgtatt acagcgttgc
     3241 tcaccaaatt tgcaaagaca ttccaattcc ttggggccca tttttgatca tgaagattta
     3301 ctgaagcgaa aaagaaaaat actatcttca gatgattcac tcaggtcatc aaaacttcaa
     3361 tcccatatga ggcattcaga cagcatttct tctctggctt ctgagagaga atatattaca
     3421 tcactagacc tttcagcaaa tgaactaaga gatattgatg ccctaagcca gaaatgctgt
     3481 ataagtgttc atttggagca tcttgaaaag ctggagcttc accagaatgc actcacgagc
     3541 tttccacaac agctatgtga aactctgaag agtttgacac atttggactt gcacagtaat
     3601 aaatttacat catttccttc ttatttgttg aaaatgagtt gtattgctaa tcttgatgtc
     3661 tctcgaaatg acattggacc ctcagtggtt ttagatccta cagtgaaatg tccaactctg
     3721 aaacagttta acctgtcata taaccagctg tcttttgtac ctgagaacct cactgatgtg
     3781 gtagagaaac tggagcagct cattttagaa ggaaataaaa tatcagggat atgctccccc
     3841 ttgagactga aggaactgaa gattttaaac cttagtaaga accacatttc atccctatca
     3901 gagaactttc ttgaggcttg tcctaaagtg gagagtttca gtgccagaat gaattttctt
     3961 gctgctatgc ctttcttgcc tccttctatg acaatcctaa aattatctca gaacaaattt
     4021 tcctgtattc cagaagcaat tttaaatctt ccacacttgc ggtctttaga tatgagcagc
     4081 aatgatattc agtacctacc aggtcccgca cactggaaat ctttgaactt aagggaactc
     4141 ttatttagcc ataatcagat cagcatcttg gacttgagtg aaaaagcata tttatggtct
     4201 agagtagaga aactgcatct ttctcacaat aaactgaaag agattcctcc tgagattggc
     4261 tgtcttgaaa atctgacatc tctggatgtc agttacaact tggaactaag atcctttccc
     4321 aatgaaatgg ggaaattaag caaaatatgg gatcttcctt tggatgaact gcatcttaac
     4381 tttgatttta aacatatagg atgtaaagcc aaagacatca taaggtttct tcaacagcga
     4441 ttaaaaaagg ctgtgcctta taaccgaatg aaacttatga ttgtgggaaa tactgggagt
     4501 ggtaaaacca ccttattgca gcaattaatg aaaaccaaga aatcagatct tggaatgcaa
     4561 agtgccacag ttggcataga tgtgaaagac tggcctatcc aaataagaga caaaagaaag
     4621 agagatctcg tcctaaatgt gtgggatttt gcaggtcgtg aggaattcta tagtactcat
     4681 ccccatttta tgacgcagcg agcattgtac cttgctgtct atgacctcag caagggacag
     4741 gctgaagttg atgccatgaa gccttggctc ttcaatataa aggctggtgc ttcttcttcc
     4801 cctgtgattc tcgttggcac acatttggat gtttctgatg agaagcaacg caaagcctgc
     4861 atgagtaaaa tcaccaagga actcctgaat aagcgagggt tccctgccat acgagattac
     4921 cactttgtga atgccaccga ggaatctgat gctttggcaa aacttcggaa aaccatcata
     4981 aacgagagcc ttaatttcaa gatccgagat cagcttgttg ttggacagct gattccagac
     5041 tgctatgtag aacttgaaaa aatcatttta tcggagcgta aaaatgtgcc aattgaattt
     5101 cccgtaattg accggaaacg attattacaa ctagtgagag aaaatcagct gcagttagat
     5161 gaaaatgagc ttcctcacgc agttcacttt ctaaatgaat caggagtcct tcttcatttt
     5221 caagacccag cactgcagtt aagtgacttg tactttgtgg aacccaagtg gctttgtaaa
     5281 atcatggcac agattttgac agtgaaagtg gaaggttgtc caaaacaccc taagggaatt
     5341 atttcgcgta gagatgtgga aaaatttctt tcaaagaaaa ggaaatttcc aaagaactac
     5401 atgacacagt attttaagct cctagaaaaa ttccagattg ctttgccaat aggagaagaa
     5461 tatttgctgg ttccaagcag tttgtctgac cacaggcctg tgatagagct tccccattgt
     5521 gagaactctg aaattatcat ccgactatat gaaatgcctt attttccaat gggattttgg
     5581 tcaagattaa tcaatcgatt acttgagatt tcaccttaca tgctttcagg gagagaacga
     5641 gcacttcgcc caaacagaat gtattggcga caaggcattt acttaaattg gtctcctgaa
     5701 gcttattgtc tggtaggatc tgaagtctta gacaatcatc cagagagttt cttaaaaatt
     5761 acagttcctt cttgtagaaa aggctgtatt cttttgggcc aagttgtgga ccacattgat
     5821 tctctcatgg aagaatggtt tcctgggttg ctggagattg atatttgtgg tgaaggagaa
     5881 actctgttga agaaatgggc attatatagt tttaatgatg gtgaagaaca tcaaaaaatc
     5941 ttacttgatg acttgatgaa gaaagcagag gaaggagatc tcttagtaaa tccagatcaa
     6001 ccaaggctca ccattccaat atctcagatt gcccctgact tgattttggc tgacctgcct
     6061 agaaatatta tgttgaataa tgatgagttg gaatttgaac aagctccaga gtttctccta
     6121 ggtgatggca gttttggatc agtttaccga gcagcctatg aaggagaaga agtggctgtg
     6181 aagattttta ataaacatac atcactcagg ctgttaagac aagagcttgt ggtgctttgc
     6241 cacctccacc accccagttt gatatctttg ctggcagctg ggattcgtcc ccggatgttg
     6301 gtgatggagt tagcctccaa gggttccttg gatcgcctgc ttcagcagga caaagccagc
     6361 ctcactagaa ccctacagca caggattgca ctccacgtag ctgatggttt gagatacctc
     6421 cactcagcca tgattatata ccgagacctg aaaccccaca atgtgctgct tttcacactg
     6481 tatcccaatg ctgccatcat tgcaaagatt gctgactacg gcattgctca gtactgctgt
     6541 agaatgggga taaaaacatc agagggcaca ccagggtttc gtgcacctga agttgccaga
     6601 ggaaatgtca tttataacca acaggctgat gtttattcat ttggtttact actctatgac
     6661 attttgacaa ctggaggtag aatagtagag ggtttgaagt ttccaaatga gtttgatgaa
     6721 ttagaaatac aaggaaaatt acctgatcca gttaaagaat atggttgtgc cccatggcct
     6781 atggttgaga aattaattaa acagtgtttg aaagaaaatc ctcaagaaag gcctacttct
     6841 gcccaggtct ttgacatttt gaattcagct gaattagtct gtctgacgag acgcatttta
     6901 ttacctaaaa acgtaattgt tgaatgcatg gttgctacac atcacaacag caggaatgca
     6961 agcatttggc tgggctgtgg gcacaccgac agaggacagc tctcatttct tgacttaaat
     7021 actgaaggat acacttctga ggaagttgct gatagtagaa tattgtgctt agccttggtg
     7081 catcttcctg ttgaaaagga aagctggatt gtgtctggga cacagtctgg tactctcctg
     7141 gtcatcaata ccgaagatgg gaaaaagaga cataccctag aaaagatgac tgattctgtc
     7201 acttgtttgt attgcaattc cttttccaag caaagcaaac aaaaaaattt tcttttggtt
     7261 ggaaccgctg atggcaagtt agcaattttt gaagataaga ctgttaagct taaaggagct
     7321 gctcctttga agatactaaa tataggaaat gtcagtactc cattgatgtg tttgagtgaa
     7381 tccacaaatt caacggaaag aaatgtaatg tggggaggat gtggcacaaa gattttctcc
     7441 ttttctaatg atttcaccat tcagaaactc attgagacaa gaacaagcca actgttttct
     7501 tatgcagctt tcagtgattc caacatcata acagtggtgg tagacactgc tctctatatt
     7561 gctaagcaaa atagccctgt tgtggaagtg tgggataaga aaactgaaaa actctgtgga
     7621 ctaatagact gcgtgcactt tttaagggag gtaatggtaa aagaaaacaa ggaatcaaaa
     7681 cacaaaatgt cttattctgg gagagtgaaa accctctgcc ttcagaagaa cactgctctt
     7741 tggataggaa ctggaggagg ccatatttta ctcctggatc tttcaactcg tcgacttata
     7801 cgtgtaattt acaacttttg taattcggtc agagtcatga tgacagcaca gctaggaagc
     7861 cttaaaaatg tcatgctggt attgggctac aaccggaaaa atactgaagg tacacaaaag
     7921 cagaaagaga tacaatcttg cttgaccgtt tgggacatca atcttccaca tgaagtgcaa
     7981 aatttagaaa aacacattga agtgagaaaa gaattagctg aaaaaatgag acgaacatct
     8041 gttgagtaag agagaaatag gcggccgcga tcccgggtgg catccctgtg acccctcccc
     8101 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     8161 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     8221 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     8281 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     8341 tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag
     8401 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     8461 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     8521 ggcgtgaacc actgctccct tccctgtcct tctgatttta aaataactat accagcagga
     8581 ggacgtccag acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg
     8641 cttgcttggc actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg
     8701 tacgcggcca gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8761 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8821 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8881 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8941 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     9001 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     9061 cctcgaggaa ctgaaaaacc agaaagttaa ttccctatag tgagtcgtat taaattcgta
     9121 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9181 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9241 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9301 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    11281 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
    11341 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    11401 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    11461 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    11521 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    11581 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    11641 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    11701 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
    11761 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    11821 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    11881 cgacggccag tgccaagctg atctatacat tgaatcaata ttggcaatta gccatattag
    11941 tcattggtta tatagcataa atcaatattg gctattggcc attgcatacg ttgtatctat
    12001 atcataatat gtacatttat attggctcat gtccaatatg accgccatgt tgacattgat
    12061 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    12121 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    12181 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg ac
//
