LOCUS       sequence-366319-       16573 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Targeting vector for knocking in constitutively expressed PE2-2A-GFP
            into the human AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Li H, Busquets O, Verma Y, Syed KM, Kutnowski N, Pangilinan GR, 
            Gilbert LA, Bateup HS, Rio DC, Hockemeyer D, Soldner F
  TITLE     Highly efficient generation of isogenic pluripotent stem cell models
            using prime editing.
  JOURNAL   Elife. 2022 Sep 7;11:e79208. doi: 10.7554/eLife.79208.
  PUBMED    36069759
REFERENCE   2  (bases 1 to 16573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-09-7"; volume: "11:e79208. doi"; pages: " 
            10.7554/eLife.79208"
COMMENT     Sequence Label: AAVS1-SA-neo-CAGGS-PE2-2A-GFP
FEATURES             Location/Qualifiers
     source          1..16573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             334..1128
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             join(1146..1214,1215..2006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1364..1383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2177..2765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2666..2685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2919..2936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3053..3074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3089..3094,3095..3112,3113..3119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3127..3143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3132..3154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3151..3167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3151..3167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    3205..4001
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    4008..4033
                     /label=SA
                     /note="splice acceptor site"
     CDS             4057..4110
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4126..4929
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     enhancer        5419..5722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     enhancer        5787..6090
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6092..6369
                     /label=chicken beta-actin promoter
     intron          6370..7386
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7310..7332
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7394..7413
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        7544..7562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7612..7632
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(7633..10146,10147..10149,10150..11733)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7633..11733
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13906..13926
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13936..13992
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(13993..13995,13996..13998,13999..14709)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(13993..13995,13996..13998,13999..14709)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(14299..14318)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     14646..14667
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             14722..14742
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             14751..14768
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(14818..14835)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14824..15048
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    15261..15790
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(15953..15971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15978..15995)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15978..15994)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15987..16009)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      16135..16563
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16222..16241)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     16432..16453
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg
       61 tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca
      121 aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta
      181 tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc
      241 tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaaga
      301 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
      361 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
      421 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
      481 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
      541 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
      601 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatccca ccttgctcct
      661 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
      721 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
      781 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
      841 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
      901 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
      961 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     1021 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     1081 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tgaaaaagga
     1141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     1201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     1261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     1321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     1381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     1441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     1501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     1561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     1621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     1681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     1741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     1801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     1861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     1921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     1981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     2101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     2161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2941 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3001 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     3061 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     3121 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     3181 agcttgggat ccataataac tagttgcttt ctctgaccag cattctctcc cctgggcctg
     3241 tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct acggctggcc cagatccttc
     3301 cctgccgcct ccttcaggtt ccgtcttcct ccactccctc ttccccttgc tctctgctgt
     3361 gttgctgccc aaggatgctc tttccggagc acttccttct cggcgctgca ccacgtgatg
     3421 tcctctgagc ggatcctccc cgtgtctggg tcctctccgg gcatctctcc tccctcaccc
     3481 aaccccatgc cgtcttcact cgctgggttc ccttttcctt ctccttctgg ggcctgtgcc
     3541 atctctcgtt tcttaggatg gccttctccg acggatgtct cccttgcgtc ccgcctcccc
     3601 ttcttgtagg cctgcatcat caccgttttt ctggacaacc ccaaagtacc ccgtctccct
     3661 ggctttagcc acctctccat ctcttgcttt ctttgcctgg acaccccgtt ctcctgtgga
     3721 ttcgggtcac ctctcactcc tttcatttgg gcagctcccc tacccccctt acctctctag
     3781 tctgctcttc cagccccctg tcatggcatc ttccaggggt ccgagagctc agctagtctt
     3841 cttcctccaa cccgggcccc tatgtccact tcaggacagc atgtttgctg cctccaggga
     3901 tcctgtgtcc ccgagctggg accaccttat attcccaggg ccggttaatg tggctctggt
     3961 tctgggtact tttatctgtc ccctccaccc cacagtgggg caagcttctg acctcttctc
     4021 ttcctcccac agggcctcga gagatctggc agcggagagg gcagaggaag tcttctaaca
     4081 tgcggtgacg tggaggagaa tcccggccct aggctcgaga cgcgtatggg atcggccatt
     4141 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     4201 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     4261 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     4321 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     4381 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     4441 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     4501 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     4561 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     4621 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     4681 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     4741 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     4801 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     4861 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     4921 ttcttctgag gggatccgct gtaagtctgc agaaattgat gatctattaa acaataaaga
     4981 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
     5041 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
     5101 atgcctgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
     5161 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
     5221 ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
     5281 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagccgg
     5341 tggcggccgc tctagaacta gttattaata gtaatcaatt acggggtcat tagttcatag
     5401 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     5461 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     5521 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     5581 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     5641 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     5701 attagtcatc gctattacca tggtcctagt tattaatagt aatcaattac ggggtcatta
     5761 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5821 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5881 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5941 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     6001 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     6061 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
     6121 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
     6181 tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc crggsggggs ggggsggggs
     6241 grggggsggg gsggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc
     6301 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
     6361 cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg
     6421 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     6481 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
     6541 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
     6601 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
     6661 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     6721 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     6781 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     6841 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     6901 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggk gggggtgccg
     6961 ggcggggcgg ggccgcctcg ggccggggrg ggctcggggg aggggcgcgg cggcccccgg
     7021 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
     7081 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
     7141 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
     7201 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
     7261 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
     7321 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
     7381 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg
     7441 aagcctctgt tccacataca cttcattctc agtattgttt tgccaagttc taattccatc
     7501 agaagctggg agctccaccg cggtggctag ctagtgcggc cgctaatacg actcactata
     7561 gggagagccg ccaccatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag
     7621 aagcggaaag tcgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc
     7681 tgggccgtga tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac
     7741 accgaccggc acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa
     7801 acagccgagg ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac
     7861 cggatctgct atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc
     7921 ttccacagac tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc
     7981 atcttcggca acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac
     8041 ctgagaaaga aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc
     8101 ctggcccaca tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac
     8161 aacagcgacg tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag
     8221 gaaaacccca tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc
     8281 aagagcagac ggctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg
     8341 ttcggaaacc tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac
     8401 ctggccgagg atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac
     8461 ctgctggccc agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc
     8521 gacgccatcc tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg
     8581 agcgcctcta tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct
     8641 ctcgtgcggc agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac
     8701 ggctacgccg gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag
     8761 cccatcctgg aaaagatgga cggcaccgag gaactgctcg tgaagctgaa cagagaggac
     8821 ctgctgcgga agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga
     8881 gagctgcacg ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg
     8941 gaaaagatcg agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg
     9001 ggaaacagca gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac
     9061 ttcgaggaag tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac
     9121 ttcgataaga acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac
     9181 ttcaccgtgt ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc
     9241 gccttcctga gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg
     9301 aaagtgaccg tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc
     9361 gtggaaatct ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg
     9421 ctgaaaatta tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa
     9481 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
     9541 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
     9601 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
     9661 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
     9721 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
     9781 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
     9841 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
     9901 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
     9961 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
    10021 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
    10081 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
    10141 gtggacgcta tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg
    10201 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
    10261 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
    10321 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
    10381 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
    10441 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
    10501 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
    10561 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
    10621 atcaaaaagt accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
    10681 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
    10741 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
    10801 cggaagcggc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
    10861 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
    10921 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
    10981 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
    11041 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
    11101 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
    11161 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
    11221 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
    11281 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
    11341 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
    11401 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
    11461 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
    11521 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
    11581 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
    11641 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
    11701 acacggatcg acctgtctca gctgggaggt gactctggag gatctagcgg aggatcctct
    11761 ggcagcgaga caccaggaac aagcgagtca gcaacaccag agagcagtgg cggcagcagc
    11821 ggcggcagca gcaccctaaa tatagaagat gagtatcggc tacatgagac ctcaaaagag
    11881 ccagatgttt ctctagggtc cacatggctg tctgattttc ctcaggcctg ggcggaaacc
    11941 gggggcatgg gactggcagt tcgccaagct cctctgatca tacctctgaa agcaacctct
    12001 acccccgtgt ccataaaaca ataccccatg tcacaagaag ccagactggg gatcaagccc
    12061 cacatacaga gactgttgga ccagggaata ctggtaccct gccagtcccc ctggaacacg
    12121 cccctgctac ccgttaagaa accagggact aatgattata ggcctgtcca ggatctgaga
    12181 gaagtcaaca agcgggtgga agacatccac cccaccgtgc ccaaccctta caacctcttg
    12241 agcgggctcc caccgtccca ccagtggtac actgtgcttg atttaaagga tgcctttttc
    12301 tgcctgagac tccaccccac cagtcagcct ctcttcgcct ttgagtggag agatccagag
    12361 atgggaatct caggacaatt gacctggacc agactcccac agggtttcaa aaacagtccc
    12421 accctgttta atgaggcact gcacagagac ctagcagact tccggatcca gcacccagac
    12481 ttgatcctgc tacagtacgt ggatgactta ctgctggccg ccacttctga gctagactgc
    12541 caacaaggta ctcgggccct gttacaaacc ctagggaacc tcgggtatcg ggcctcggcc
    12601 aagaaagccc aaatttgcca gaaacaggtc aagtatctgg ggtatcttct aaaagagggt
    12661 cagagatggc tgactgaggc cagaaaagag actgtgatgg ggcagcctac tccgaagacc
    12721 cctcgacaac taagggagtt cctagggaag gcaggcttct gtcgcctctt catccctggg
    12781 tttgcagaaa tggcagcccc cctgtaccct ctcaccaaac cggggactct gtttaattgg
    12841 ggcccagacc aacaaaaggc ctatcaagaa atcaagcaag ctcttctaac tgccccagcc
    12901 ctggggttgc cagatttgac taagcccttt gaactctttg tcgacgagaa gcagggctac
    12961 gccaaaggtg tcctaacgca aaaactggga ccttggcgtc ggccggtggc ctacctgtcc
    13021 aaaaagctag acccagtagc agctgggtgg cccccttgcc tacggatggt agcagccatt
    13081 gccgtactga caaaggatgc aggcaagcta accatgggac agccactagt cattctggcc
    13141 ccccatgcag tagaggcact agtcaaacaa ccccccgacc gctggctttc caacgcccgg
    13201 atgactcact atcaggcctt gcttttggac acggaccggg tccagttcgg accggtggta
    13261 gccctgaacc cggctacgct gctcccactg cctgaggaag ggctgcaaca caactgcctt
    13321 gatatcctgg ccgaagccca cggaacccga cccgacctaa cggaccagcc gctcccagac
    13381 gccgaccaca cctggtacac ggatggaagc agtctcttac aagagggaca gcgtaaggcg
    13441 ggagctgcgg tgaccaccga gaccgaggta atctgggcta aagccctgcc agccgggaca
    13501 tccgctcagc gggctgaact gatagcactc acccaggccc taaagatggc agaaggtaag
    13561 aagctaaatg tttatactga tagccgttat gcttttgcta ctgcccatat ccatggagaa
    13621 atatacagaa ggcgtgggtg gctcacatca gaaggcaaag agatcaaaaa taaagacgag
    13681 atcttggccc tactaaaagc cctctttctg cccaaaagac ttagcataat ccattgtcca
    13741 ggacatcaaa agggacacag cgccgaggct agaggcaacc ggatggctga ccaagcggcc
    13801 cgaaaggcag ccatcacaga gactccagac acctctaccc tcctcataga aaattcatca
    13861 ccctctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg
    13921 aaagtcggaa gcggagctac taacttcagc ctgctgaagc aggctggaga cgtggaggag
    13981 aaccctggac ctatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
    14041 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
    14101 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
    14161 ccctggccca ccctcgtgac caccctgacc tatggagtgc agtgcttcag ccgctacccc
    14221 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
    14281 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
    14341 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
    14401 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
    14461 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
    14521 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
    14581 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
    14641 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
    14701 ctgtacaagt ctggtggttc tcccaagaag aagaggaaag tctaaccggt catcatcacc
    14761 atcaccattg agtttaattc ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
    14821 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    14881 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    14941 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    15001 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
    15061 aaagaaccgt accacaagct ttactaggga caggattggt gacagaaaag ccccatcctt
    15121 aggcctcctc cttcctagtc tcctgatatt gggtctaacc cccacctcct gttaggcaga
    15181 ttccttatct ggtgacacac ccccatttcc tggagccatc tctctccttg ccagaacctc
    15241 taaggtttgc ttacgatgga gccagagagg atcctgggag ggagagcttg gcagggggtg
    15301 ggagggaagg gggggatgcg tgacctgccc ggttctcagt ggccaccctg cgctaccctc
    15361 tcccagaacc tgagctgctc tgacgcggct gtctggtgcg tttcactgat cctggtgctg
    15421 cagcttcctt acacttccca agaggagaag cagtttggaa aaacaaaatc agaataagtt
    15481 ggtcctgagt tctaactttg gctcttcacc tttctagtcc ccaatttata ttgttcctcc
    15541 gtgcgtcagt tttacctgtg agataaggcc agtagccagc cccgtcctgg cagggctgtg
    15601 gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt
    15661 tcaccaggtc gtggccgcct ctactccctt tctctttctc catccttctt tccttaaaga
    15721 gtccccagtg ctatctggga catattcctc cgcccagagc agggtcccgc ttccctaagg
    15781 ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg ctcaggcttc
    15841 cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc ttccctacag
    15901 gggttcctgg ctctgcggcc gctcgagcat gcatctagag ggcccaattc gccctatagt
    15961 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    16021 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    16081 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
    16141 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    16201 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    16261 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    16321 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    16381 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    16441 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    16501 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    16561 attttaacaa aat
//
