LOCUS       sequence-366338-        4946 bp    DNA     circular SYN 15-APR-2025
DEFINITION  ISH probe for X laevis foxd4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Sullivan SA, Akers L, Moody SA
  TITLE     foxD5a, a Xenopus winged helix gene, maintains an immature neural 
            ectoderm via transcriptional repression that is dependent on the 
            C-terminal domain.
  JOURNAL   Dev Biol. 2001 Apr 15. 232(2):439-57.
  PUBMED    11401404
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2001 Apr 15. 232(2):439-57."
COMMENT     Sequence Label: pBS-foxd4l1.1
FEATURES             Location/Qualifiers
     source          1..4946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2702..2718)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2702..2718)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2755..2773)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2794..2810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2794..2810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2807..2829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2818..2834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2842..2848,2849..2866,2867..2872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2887..2908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3025..3042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3196..3784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3276..3295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3955..4746,4747..4815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4578..4597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4816..4920)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc
      661 ccctcgagtt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      721 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      781 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      841 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      901 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tgtatattca
      961 ggtgaattat tttatttagc ataagtagac tgaaaagttg caataacttt tataataaat
     1021 agttgaatag attacatttc tatttacatt atatattaac agtaaaataa gacaaaacaa
     1081 aaggatggcg ttacaaaact gcatacccgt tgcccactgg ggccaagtga catcctaagc
     1141 catcctaagc cacaatttca cacacatata cataaaatcc atagtgattc aagcgtaatt
     1201 caagcctcca gcagcacatg tgccacgttt gggttgtcat acaagtacat atgactgtct
     1261 ggttatgtat aaccagcggt cctctagctg ttattgaact gcaacttccg tccttcccgg
     1321 acaaccttgt aatttagggg gtgctagaaa ttttacttta gtacaaagtg gagggtcaca
     1381 gattggataa ccttgtcttc gacaataaat aaaaaataaa attaaagtct ttggatttgg
     1441 acacaagaag gtcctagttg tagaatccat gagcaataac atattaagaa aatgggaggg
     1501 cagtactgga gttgtatgag acccaaagtc acttacttca gtaacaccca gggaattgta
     1561 tgaggttgta ttgcctggca ccctgagtat tggctagcag agggccagtg gataagttaa
     1621 gcacagctgg gagaaggcag gaacttggcc tctggaatac atgattatag ttccagtgtg
     1681 agttgaagct ttgaatattt ggctctggct ccttgggttt cctcatgatg ttctcaatac
     1741 tgaatgaaca tttgctctgt gaattattgg gatggttatc agcatcctgg gctttaaaac
     1801 ccctgtgtac cccctgggca gggtaagaaa ccttcctctt tatgtctggg tagggtgcaa
     1861 ggtgagttga catgggcaag gtctctggta ttgccatgca tgtcagtgaa gtctgctgga
     1921 gcactggctg aggttggata gcactgtagg gtctgtagta aggcagagag ttgtacatca
     1981 tgagtccatc cttaaagaac tcctgttggt gcctcttaaa ccttttcctc cttctaagga
     2041 agcttccgtt atcaaacatg tcctcagaag cagggtccag tgtccaataa ttgccttttc
     2101 ctggatttcc tggttcccgt ggtattttaa taaagcagtc attaagggac aggttgtgcc
     2161 tgatggaatt ctgccaagca gggaacttgt ccttgtagta agggaacttg ctgctgataa
     2221 aatcacagat gccactgaga gtcagtttct tgtggggact ctgcaggata gccatggtga
     2281 ttagagcaat gtaagagtaa ggaggcttta ccagagtcct tttggctttg ctaccagttg
     2341 gggtggttga ggagtcttta gaagtacctc cttcactctc tccagaggaa tcactctcac
     2401 tttcagtgca gctgagcttg gaaggactca gcatctcact gtcccccatc actgaatgtg
     2461 tgggctgtag gtaaaagtgt gactttaggc tgcagggatc atcttctcca agaatatcaa
     2521 tttcatcctc ctcatcagaa agtcctgcat aatcctgggg gtgatgatgg gcaccagact
     2581 cctggctaaa gctcattgtt agcactaatg cttggttggc agtaagtagt agacagcagg
     2641 atctccagtg agagttgtgc ttccccactg ctctctcgtg ccgaattcct gcagcccggg
     2701 ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta
     2761 gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     2881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3001 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4921 cacatttccc cgaaaagtgc cacctg
//
